##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064185_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	165154
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.59976143478208	32.0	32.0	32.0	21.0	32.0
2	28.628056238419898	32.0	32.0	32.0	14.0	32.0
3	30.32558097291013	32.0	32.0	32.0	21.0	32.0
4	30.8576359034598	32.0	32.0	32.0	32.0	32.0
5	30.389830097969167	32.0	32.0	32.0	21.0	32.0
6	34.12452619978929	36.0	36.0	36.0	32.0	36.0
7	34.15365053223052	36.0	36.0	36.0	32.0	36.0
8	33.989204015646	36.0	36.0	36.0	32.0	36.0
9	34.46344018310183	36.0	36.0	36.0	32.0	36.0
10	33.721393366191556	36.0	36.0	36.0	21.0	36.0
11	34.549686958838414	36.0	36.0	36.0	32.0	36.0
12	34.18173946740618	36.0	36.0	36.0	32.0	36.0
13	34.3855976845853	36.0	36.0	36.0	32.0	36.0
14	34.22187170761834	36.0	36.0	36.0	32.0	36.0
15	34.09547452680528	36.0	36.0	36.0	32.0	36.0
16	34.13927606960776	36.0	36.0	36.0	32.0	36.0
17	34.09324024849534	36.0	36.0	36.0	32.0	36.0
18	34.11453552441963	36.0	36.0	36.0	32.0	36.0
19	34.12287319713722	36.0	36.0	36.0	32.0	36.0
20	34.154831248410574	36.0	36.0	36.0	32.0	36.0
21	34.16761931288373	36.0	36.0	36.0	32.0	36.0
22	34.101874613996635	36.0	36.0	36.0	32.0	36.0
23	34.03176429272073	36.0	36.0	36.0	32.0	36.0
24	33.96833864151035	36.0	36.0	36.0	32.0	36.0
25	33.920892015936644	36.0	36.0	36.0	32.0	36.0
26	33.81468810927982	36.0	36.0	36.0	32.0	36.0
27	33.82319532073095	36.0	36.0	36.0	32.0	36.0
28	33.7503965995374	36.0	36.0	36.0	32.0	36.0
29	33.69746418494254	36.0	36.0	36.0	32.0	36.0
30	33.67523644598375	36.0	36.0	36.0	27.0	36.0
31	33.714539157392494	36.0	36.0	36.0	32.0	36.0
32	33.62542233309517	36.0	36.0	36.0	27.0	36.0
33	33.560694866609346	36.0	36.0	36.0	21.0	36.0
34	33.52979643242065	36.0	36.0	36.0	21.0	36.0
35	33.5351247926178	36.0	36.0	36.0	21.0	36.0
36	33.42749797159015	36.0	36.0	36.0	21.0	36.0
37	33.45001634837788	36.0	36.0	36.0	21.0	36.0
38	33.37478959032176	36.0	36.0	36.0	21.0	36.0
39	33.38849195296511	36.0	36.0	36.0	21.0	36.0
40	33.349558593797305	36.0	36.0	36.0	21.0	36.0
41	33.282663453504	36.0	36.0	36.0	21.0	36.0
42	33.205820022524435	36.0	36.0	36.0	21.0	36.0
43	33.23918887825908	36.0	36.0	36.0	21.0	36.0
44	33.07538418688012	36.0	36.0	36.0	21.0	36.0
45	33.01005122491735	36.0	36.0	36.0	14.0	36.0
46	33.02831296850213	36.0	36.0	36.0	14.0	36.0
47	33.031697688218266	36.0	36.0	36.0	14.0	36.0
48	32.91914818896303	36.0	36.0	36.0	14.0	36.0
49	32.864708090630565	36.0	36.0	36.0	14.0	36.0
50	31.9639427443477	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	11.0
22	22.0
23	60.0
24	166.0
25	444.0
26	948.0
27	1754.0
28	3322.0
29	5723.0
30	8971.0
31	13907.0
32	21001.0
33	31330.0
34	43055.0
35	34437.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.3517732471889	19.192365681899375	12.556539851891321	22.8993212190204
2	17.867674217240985	22.2333230398479	36.549744782111134	23.349257960799985
3	20.36523487169551	25.52163435339138	29.830340167358948	24.282790607554162
4	15.227000254308102	18.20422151446529	37.88706298363952	28.681715247587103
5	18.85512915218523	34.26377804957797	31.376775615486153	15.504317182750645
6	33.19689501919421	38.404761616430726	15.929980503045643	12.468362861329425
7	29.17579955677731	31.65893650774429	20.468169102776802	18.697094832701598
8	27.864296353706237	33.53415599985468	18.65168267193044	19.949864974508642
9	26.846015512888048	13.712739096474177	18.1982767494384	41.242968641199376
10	15.054434043377697	26.62605810334597	31.611102365065335	26.708405488211
11	37.19740363539484	21.200818629884836	22.27194012860724	19.32983760611308
12	24.002446201726872	23.95037359070928	28.82158470276227	23.22559550480158
13	29.114391590776982	19.687923851966673	25.3481641154815	25.84952044177485
14	23.599186214078983	19.610787507417317	25.499231020744272	31.290795257759424
15	25.111569972690333	27.114076890936943	22.32852739746765	25.44582573890507
16	25.375104447969775	26.595177834021577	23.636726933649804	24.39299078435884
17	23.458105767949913	26.503748017002316	25.32666480981387	24.711481405233904
18	24.300955471862626	25.451396878065317	26.253678385022468	23.99396926504959
19	24.862400319703546	25.83301544628314	25.998316712381854	23.306267521631458
20	25.02815553967812	24.623684561076328	25.290940576673894	25.05721932257166
21	26.254889375976358	24.884653111641256	24.750233115758626	24.110224396623757
22	25.12382382503603	24.929459776935467	25.71115443767635	24.235561960352157
23	24.199837727212177	24.50864042045606	26.153166135848966	25.138355716482796
24	23.910410889230658	25.908545963161657	25.773520471802076	24.407522675805613
25	24.347267971323387	24.849229800426272	26.046308854873086	24.757193373377255
26	24.37934458788481	25.547387797611837	26.01424177101751	24.05902584348584
27	24.865725686812354	25.177569074824248	25.620810550600375	24.335894687763023
28	23.614609571788414	25.134421623716335	26.772912226312727	24.47805657818252
29	24.056312443233423	25.43082046624281	26.03693611867999	24.47593097184378
30	24.0288954688853	25.566919168982782	26.653224097319356	23.750961264812563
31	24.970782543402304	25.355907981664156	25.44673945295233	24.226570021981217
32	24.780652622783062	25.40009324912655	25.353468685853382	24.46578544223701
33	23.993654330556094	25.118073047858942	26.21645514435187	24.671817477233095
34	24.356940441301138	24.988495167970548	26.641557875360284	24.013006515368033
35	25.690739877322905	24.748560390919717	25.8148702081151	23.745829523642286
36	24.203833227359432	25.72137947739728	25.525176370848097	24.54961092439519
37	25.45777121127716	25.233729745440453	25.678179572262454	23.630319471019934
38	24.406268544646426	24.938537743275486	26.093907061801357	24.56128665027673
39	24.709501232235525	25.15516479257873	25.331371444833994	24.803962530351747
40	25.002724795640326	25.731153496821076	25.77838328792007	23.487738419618527
41	23.88659800056919	25.816393879392297	26.136109042247206	24.1608990777913
42	25.42506297229219	26.135317767874444	25.29306335981399	23.146555900019376
43	24.558439749807754	24.922344734882202	26.10730655816122	24.411908957148825
44	24.440972916023323	25.818483466845894	25.116709959855406	24.623833657275377
45	24.398587975998016	26.212057909938057	25.456394979201104	23.932959134862823
46	24.00971214745026	25.91641740436199	25.192244813931243	24.881625634256512
47	24.396003633060854	25.386618225855283	26.004844081138355	24.212534059945504
48	25.23402966999697	26.283378746594003	24.775052982137453	23.707538601271573
49	24.266736097655492	25.954877930633597	25.156825227669056	24.62156074404185
50	25.222066810775463	25.463660968919726	24.79155691994696	24.52271530035785
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	6.0
2	8.0
3	15.5
4	23.0
5	28.0
6	33.0
7	33.0
8	33.0
9	46.0
10	59.0
11	69.0
12	79.0
13	168.5
14	258.0
15	360.5
16	463.0
17	517.0
18	571.0
19	605.0
20	639.0
21	744.0
22	849.0
23	1013.5
24	1178.0
25	1390.0
26	1602.0
27	1942.0
28	2282.0
29	2568.5
30	2855.0
31	3147.0
32	3439.0
33	3800.0
34	4161.0
35	4708.0
36	5255.0
37	5966.5
38	6678.0
39	7319.5
40	7961.0
41	8625.0
42	9289.0
43	9359.0
44	9429.0
45	10019.5
46	10610.0
47	11103.5
48	11597.0
49	11791.0
50	11985.0
51	11345.0
52	10705.0
53	10443.5
54	10182.0
55	10402.0
56	10622.0
57	10499.5
58	10377.0
59	9194.0
60	8011.0
61	7161.0
62	6311.0
63	5571.0
64	4831.0
65	4199.5
66	3568.0
67	3129.0
68	2690.0
69	2505.5
70	2321.0
71	1808.0
72	1295.0
73	1129.0
74	963.0
75	793.5
76	624.0
77	517.5
78	411.0
79	367.0
80	323.0
81	263.0
82	203.0
83	182.5
84	162.0
85	126.5
86	91.0
87	68.0
88	45.0
89	33.5
90	22.0
91	19.0
92	16.0
93	16.5
94	17.0
95	14.0
96	11.0
97	7.5
98	4.0
99	6.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.003027477384743936
2	6.054954769487872E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	6.054954769487872E-4
10	0.0
11	0.0
12	0.0
13	0.0012109909538975743
14	0.0
15	0.006660450246436659
16	0.0
17	0.0
18	0.0
19	6.054954769487872E-4
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0012109909538975743
26	0.003632972861692723
27	0.00423846833864151
28	0.0012109909538975743
29	0.0024219819077951486
30	0.00423846833864151
31	0.007871441200334233
32	0.003027477384743936
33	0.0012109909538975743
34	0.003632972861692723
35	0.003027477384743936
36	0.011504414062026957
37	0.003632972861692723
38	0.007265945723385446
39	0.00423846833864151
40	0.0024219819077951486
41	0.00423846833864151
42	0.0012109909538975743
43	6.054954769487872E-4
44	6.054954769487872E-4
45	6.054954769487872E-4
46	0.0
47	0.0024219819077951486
48	0.0024219819077951486
49	0.0012109909538975743
50	6.054954769487872E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	165154.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.33110665990002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.62774232034036	63.21914940478332
2	7.24676134303897	10.33839012598306
3	1.6357157120537602	3.5003223576534315
4	0.6790559114000906	1.937512385764619
5	0.37882317504741553	1.3510938152274583
6	0.2212306880289721	0.9468377882542605
7	0.17537608719329667	0.8756839266826679
8	0.13352267100149495	0.7619455909377899
9	0.1066184515981924	0.6844690928577645
>10	0.6925210307185323	9.664295802796916
>50	0.07672534890849636	3.781279159406907
>100	0.025907260670401364	2.9390205496517967
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	490	0.2966927837049057	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	286	0.17317170640735313	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	187	0.1132276541894232	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	184	0.11141116775857685	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	182	0.11020017680467926	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	178	0.10777819489688412	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	176	0.10656720394298655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	6.054954769487872E-4
9	0.0	0.0	0.0	0.0	6.054954769487872E-4
10	0.0	0.0	0.0	0.0	6.054954769487872E-4
11	0.0	0.0	0.0	0.0	6.054954769487872E-4
12	0.0	0.0	0.0	0.0	6.054954769487872E-4
13	0.0	0.0	0.0	0.0	6.054954769487872E-4
14	0.0	0.0	0.0	0.0	6.054954769487872E-4
15	0.0	0.0	0.0	0.0	6.054954769487872E-4
16	0.0	0.0	0.0	0.0	6.054954769487872E-4
17	0.0	0.0	0.0	0.0	6.054954769487872E-4
18	0.0	0.0	0.0	0.0	6.054954769487872E-4
19	0.0	0.0	0.0	0.0	6.054954769487872E-4
20	0.0	0.0	0.0	0.0	6.054954769487872E-4
21	0.0	0.0	0.0	0.0	6.054954769487872E-4
22	0.0	0.0	0.0	0.0	6.054954769487872E-4
23	0.0	0.0	0.0	0.0	6.054954769487872E-4
24	0.0	0.0	0.0	0.0012109909538975743	6.054954769487872E-4
25	0.0	0.0	0.0	0.0012109909538975743	6.054954769487872E-4
26	0.0	0.0	0.0	0.003027477384743936	6.054954769487872E-4
27	0.0	0.0	0.0	0.003027477384743936	6.054954769487872E-4
28	0.0	0.0	0.0	0.003027477384743936	6.054954769487872E-4
29	0.0	0.0	0.0	0.003027477384743936	6.054954769487872E-4
30	0.0	0.0	0.0	0.00423846833864151	6.054954769487872E-4
31	0.0	0.0	0.0	0.00847693667728302	6.054954769487872E-4
32	0.0	0.0	0.0	0.013320900492873319	6.054954769487872E-4
33	0.0	0.0	0.0	0.01937585526236119	6.054954769487872E-4
34	0.0	0.0	0.0	0.026641800985746637	0.0018164864308463616
35	0.0	0.0	0.0	0.03451324218608087	0.0018164864308463616
36	0.0	0.0	0.0	0.0423846833864151	0.0018164864308463616
37	0.0	0.0	0.0	0.06297152960267387	0.0018164864308463616
38	0.0	0.0	0.0	0.08840233963452293	0.0018164864308463616
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATA	35	7.252407E-6	37.714287	36
ATAATAC	25	0.002346934	35.2	3
GATGCGG	50	5.893162E-8	35.2	34
ATGCGGC	50	5.893162E-8	35.2	35
GGCGTTA	55	1.3615681E-7	32.0	42
CGTTATT	55	1.3615681E-7	32.0	44
TGCGGCG	60	2.9194234E-7	29.333332	36
AGACTGT	40	7.0062815E-4	27.5	6
TCGCACG	40	7.0062815E-4	27.5	13
GATCGCA	40	7.0062815E-4	27.5	11
CCGATCG	40	7.0062815E-4	27.5	9
CGATCGC	40	7.0062815E-4	27.5	10
GCGTTAT	65	5.87921E-7	27.076923	43
GCGATGC	65	5.87921E-7	27.076923	32
CGATGCG	65	5.87921E-7	27.076923	33
TAGCGGC	50	8.298695E-5	26.400002	30
ATACGAA	50	8.298695E-5	26.400002	40
CAATACG	50	8.298695E-5	26.400002	38
CTAGCGG	50	8.298695E-5	26.400002	29
GGATGAA	45	0.0013931147	24.444445	1
>>END_MODULE