FastQCFastQC Report
Thu 2 Feb 2017
SRR4064184_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064184_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623216
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32000.19713950577125902No Hit
TATCAACGCAGAGTACTTTTTTTTT22000.13553341021774057No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG16550.10195808814107304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG2750.013.47371113
AACCGCG1001.0053554E-813.3017927
GCATCGC2900.013.10400214
ACCGTCG2150.012.815688
CGCCAGT2900.012.77679518
CGTCGTA2150.012.37375910
TCGCCAG3150.012.36483917
GCTAGTC853.937679E-612.29577357
CCGTTAA853.9444712E-612.29387816
GCGTTAT853.9526367E-612.2916041
CGACCCG1650.012.0906735
ATACCGT2250.011.8227216
ATCGCCA3400.011.73506516
TAGAGTG2400.011.4789435
GTTTTAT1501.7644197E-1011.397671
ATTAGAC752.0802436E-411.3962653
ACTCTAA2800.011.19793610
GTATCAA20500.011.166011
CACTCTA2900.011.1394329
ACGGTAT2900.011.1394329
Produced by FastQC (version 0.11.5)