##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064184_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1623216
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.237863599175956	32.0	32.0	32.0	32.0	32.0
2	30.814271175247164	32.0	32.0	32.0	32.0	32.0
3	30.82679507841224	32.0	32.0	32.0	32.0	32.0
4	30.867867246256814	32.0	32.0	32.0	32.0	32.0
5	30.723412657341967	32.0	32.0	32.0	32.0	32.0
6	34.38494322382234	36.0	36.0	36.0	32.0	36.0
7	34.33463876649811	36.0	36.0	36.0	32.0	36.0
8	34.280288636878886	36.0	36.0	36.0	32.0	36.0
9	34.465098298686065	36.0	36.0	36.0	32.0	36.0
10	34.09136738425447	36.0	36.0	36.0	32.0	36.0
11	34.427943046396784	36.0	36.0	36.0	32.0	36.0
12	34.21894744753625	36.0	36.0	36.0	32.0	36.0
13	34.316705848143435	36.0	36.0	36.0	32.0	36.0
14	34.20429258952598	36.0	36.0	36.0	32.0	36.0
15	34.1228856788006	36.0	36.0	36.0	32.0	36.0
16	34.12862490266237	36.0	36.0	36.0	32.0	36.0
17	34.04635550659925	36.0	36.0	36.0	32.0	36.0
18	34.040590408177344	36.0	36.0	36.0	32.0	36.0
19	34.06049903401642	36.0	36.0	36.0	32.0	36.0
20	34.04089412622842	36.0	36.0	36.0	32.0	36.0
21	34.033583946930044	36.0	36.0	36.0	32.0	36.0
22	34.00249627899183	36.0	36.0	36.0	32.0	36.0
23	33.94752146356369	36.0	36.0	36.0	32.0	36.0
24	33.92706084710846	36.0	36.0	36.0	32.0	36.0
25	33.50675387625553	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	2.0
4	14.0
5	18.0
6	59.0
7	21.0
8	84.0
9	111.0
10	126.0
11	58.0
12	94.0
13	72.0
14	159.0
15	268.0
16	412.0
17	621.0
18	795.0
19	1034.0
20	1517.0
21	2258.0
22	3609.0
23	5581.0
24	8459.0
25	12424.0
26	18124.0
27	24602.0
28	33970.0
29	46287.0
30	61415.0
31	84970.0
32	119801.0
33	168867.0
34	349020.0
35	678363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.47050180943272	17.052054470334177	11.27709218739215	26.20035153284096
2	16.814486964171955	19.475129101458776	37.18436218149736	26.5260217528719
3	18.84946096783673	23.458295316559337	28.46320232973773	29.2290413858662
4	12.62631241074498	15.170611856639956	35.578545033816795	36.62453069879827
5	14.837738611082187	36.337272525077154	33.54303609594286	15.281952767897794
6	34.76335034081716	35.13740818762165	16.69538900229265	13.403852469268537
7	30.64919043757533	30.182419592174764	20.60076950948084	18.567620460769067
8	28.453150180102732	32.65162369776019	19.158678363329912	19.736547758807163
9	27.4916054849096	13.906296995725084	18.182877161495185	40.41922035787013
10	15.824514643299192	26.44343381544364	31.712149081064556	26.01990246019261
11	37.46626840059518	20.855343368882238	22.305016254038854	19.373371976483728
12	24.732020616027413	23.328831308449526	28.77287048161392	23.166277593909136
13	29.635399072377716	19.25167610645218	25.048144138990462	26.064780682179638
14	23.72848861150907	19.219326775837708	25.08143442956446	31.970750183088764
15	25.31983970875863	26.883918296094983	22.029690001275966	25.76655199387042
16	25.991356166976022	25.473231672848613	23.495420100024845	25.039992060150524
17	24.215884616285535	25.745650857993162	25.00477724478109	25.03368728094021
18	25.031534947380102	24.84688750377618	25.65754835050339	24.46402919834033
19	25.765416526412743	24.780988102768212	25.102431602322483	24.351163768496566
20	25.958149915535333	24.137227339424655	24.643583767987277	25.261038977052735
21	26.898309839183494	24.037644070565676	24.21199295946142	24.852053130789407
22	26.012617685845147	23.98611844940441	24.643204815756263	25.358059048994182
23	24.81263132454055	23.99089729804382	25.081322567598228	26.115148809817402
24	25.07180891891742	24.702144804018218	24.843635655201286	25.382410621863077
25	25.116738450384158	24.210081121971488	25.073211993573373	25.59996843407098
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	112.0
1	112.0
2	308.0
3	504.0
4	504.0
5	504.0
6	1341.5
7	2179.0
8	2179.0
9	2179.0
10	2358.5
11	2538.0
12	2538.0
13	2538.0
14	3045.5
15	3553.0
16	3553.0
17	3553.0
18	6241.5
19	8930.0
20	8930.0
21	8930.0
22	14680.0
23	20430.0
24	20430.0
25	20430.0
26	30694.5
27	40959.0
28	40959.0
29	40959.0
30	52019.0
31	63079.0
32	63079.0
33	63079.0
34	80318.5
35	97558.0
36	97558.0
37	97558.0
38	113313.5
39	129069.0
40	129069.0
41	129069.0
42	149114.0
43	169159.0
44	169159.0
45	169159.0
46	189886.0
47	210613.0
48	210613.0
49	210613.0
50	217689.5
51	224766.0
52	224766.0
53	224766.0
54	211336.5
55	197907.0
56	197907.0
57	197907.0
58	182024.5
59	166142.0
60	166142.0
61	166142.0
62	145163.5
63	124185.0
64	124185.0
65	124185.0
66	101562.0
67	78939.0
68	78939.0
69	78939.0
70	59494.5
71	40050.0
72	40050.0
73	40050.0
74	30388.0
75	20726.0
76	20726.0
77	20726.0
78	16460.0
79	12194.0
80	12194.0
81	12194.0
82	8445.0
83	4696.0
84	4696.0
85	4696.0
86	3559.5
87	2423.0
88	2423.0
89	2423.0
90	1762.5
91	1102.0
92	1102.0
93	1102.0
94	777.0
95	452.0
96	452.0
97	452.0
98	701.5
99	951.0
100	951.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037456506096539215
2	0.004558851070960365
3	0.0017865767710520352
4	0.0035731535421040703
5	0.007700761944189806
6	0.01262924958847128
7	0.022424618781480717
8	0.0327128367389183
9	0.044849237562961435
10	0.05569191038038068
11	0.052365181220490685
12	0.06055879192910864
13	0.06185251993573252
14	0.0648096125223014
15	0.05661600181368345
16	0.0627766113690353
17	0.058032942011414373
18	0.07429695123754325
19	0.07133985865097436
20	0.0761451341041488
21	0.07275679884870528
22	0.07435855733309676
23	0.07885580230850361
24	0.07411213295088269
25	0.0747898000019714
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1623216.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.2539278457201
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.35334642750684	41.96722875702946
2	13.346661941494386	14.4821766791011
3	4.0619787037705155	6.611348985156515
4	1.7324164874279124	3.759615964305993
5	0.9299299812959398	2.5226177053400876
6	0.5658136960052954	1.84185692623149
7	0.36796457901949076	1.3974466603932956
8	0.27938066027273273	1.2125998547141184
9	0.20101260820701333	0.9815151187568976
>10	1.0094933442993534	9.75075498933336
>50	0.07226006206572592	2.71503601888261
>100	0.0692464312432029	7.860907574227268
>500	0.007643154187481033	2.7942645431578383
>1k	0.002851923204283968	2.1026302233698333
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3200	0.19713950577125902	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2200	0.13553341021774057	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1655	0.10195808814107304	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	3.0803047776759224E-4
13	0.0	0.0	0.0	0.0	3.0803047776759224E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	275	0.0	13.473711	13
AACCGCG	100	1.0053554E-8	13.301792	7
GCATCGC	290	0.0	13.104002	14
ACCGTCG	215	0.0	12.81568	8
CGCCAGT	290	0.0	12.776795	18
CGTCGTA	215	0.0	12.373759	10
TCGCCAG	315	0.0	12.364839	17
GCTAGTC	85	3.937679E-6	12.2957735	7
CCGTTAA	85	3.9444712E-6	12.293878	16
GCGTTAT	85	3.9526367E-6	12.291604	1
CGACCCG	165	0.0	12.090673	5
ATACCGT	225	0.0	11.822721	6
ATCGCCA	340	0.0	11.735065	16
TAGAGTG	240	0.0	11.478943	5
GTTTTAT	150	1.7644197E-10	11.39767	1
ATTAGAC	75	2.0802436E-4	11.396265	3
ACTCTAA	280	0.0	11.197936	10
GTATCAA	2050	0.0	11.16601	1
CACTCTA	290	0.0	11.139432	9
ACGGTAT	290	0.0	11.139432	9
>>END_MODULE