FastQCFastQC Report
Thu 2 Feb 2017
SRR4064184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623216
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA4000.022.00015410
ACCGTCG3850.021.71318
TACCGTC4200.021.475027
GTATCAA28350.020.8762131
ATTAGAC1301.6279955E-920.3059583
ATACCGT4350.020.2281576
CCGTCGT4550.018.3737559
TAGGACG1852.1827873E-1117.8363154
GTCGTAG4850.017.69084511
GGTATCA15150.017.4269391
CGTCTTA1903.274181E-1117.36800815
TATAGGA2300.017.215922
GTACTAA902.2147984E-417.1122861
CGCAATA4650.017.03342636
GTATATG2109.094947E-1216.7630561
AAGACGG5950.016.6372345
AACGCAG35200.016.6235796
CAAGACG5900.016.4053784
CGCTAGA5650.016.354637
GTAATAC1753.6325218E-916.3414613
Produced by FastQC (version 0.11.5)