##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064184_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1623216
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.242956575095366	32.0	32.0	32.0	32.0	32.0
2	31.291323520714432	32.0	32.0	32.0	32.0	32.0
3	31.405376117534573	32.0	32.0	32.0	32.0	32.0
4	31.513052483464925	32.0	32.0	32.0	32.0	32.0
5	31.42560324688766	32.0	32.0	32.0	32.0	32.0
6	35.03603217316734	36.0	36.0	36.0	36.0	36.0
7	35.058336660062494	36.0	36.0	36.0	36.0	36.0
8	34.99872660200491	36.0	36.0	36.0	36.0	36.0
9	35.14093503267587	36.0	36.0	36.0	36.0	36.0
10	34.95630649278962	36.0	36.0	36.0	36.0	36.0
11	35.13896055731338	36.0	36.0	36.0	36.0	36.0
12	35.032771362529694	36.0	36.0	36.0	36.0	36.0
13	35.08087463405979	36.0	36.0	36.0	36.0	36.0
14	35.02294580634987	36.0	36.0	36.0	36.0	36.0
15	34.98656124631596	36.0	36.0	36.0	36.0	36.0
16	34.99839454514988	36.0	36.0	36.0	36.0	36.0
17	34.96252501207479	36.0	36.0	36.0	36.0	36.0
18	34.96582709879646	36.0	36.0	36.0	36.0	36.0
19	34.968324609910205	36.0	36.0	36.0	36.0	36.0
20	34.95687080462489	36.0	36.0	36.0	36.0	36.0
21	34.95765813052607	36.0	36.0	36.0	36.0	36.0
22	34.93266576968192	36.0	36.0	36.0	36.0	36.0
23	34.887521438921254	36.0	36.0	36.0	32.0	36.0
24	34.85565876630097	36.0	36.0	36.0	32.0	36.0
25	34.828800356822505	36.0	36.0	36.0	32.0	36.0
26	34.76075765640555	36.0	36.0	36.0	32.0	36.0
27	34.757109343426876	36.0	36.0	36.0	32.0	36.0
28	34.73783834067678	36.0	36.0	36.0	32.0	36.0
29	34.70637857192142	36.0	36.0	36.0	32.0	36.0
30	34.69673536978443	36.0	36.0	36.0	32.0	36.0
31	34.69504120215671	36.0	36.0	36.0	32.0	36.0
32	34.648006796384465	36.0	36.0	36.0	32.0	36.0
33	34.624865698711695	36.0	36.0	36.0	32.0	36.0
34	34.61631600477077	36.0	36.0	36.0	32.0	36.0
35	34.5736020344797	36.0	36.0	36.0	32.0	36.0
36	34.552394752146355	36.0	36.0	36.0	32.0	36.0
37	34.545993878818344	36.0	36.0	36.0	32.0	36.0
38	34.50272976609398	36.0	36.0	36.0	32.0	36.0
39	34.50220118579413	36.0	36.0	36.0	32.0	36.0
40	34.482619072261485	36.0	36.0	36.0	32.0	36.0
41	34.46908544519029	36.0	36.0	36.0	32.0	36.0
42	34.39472319149146	36.0	36.0	36.0	32.0	36.0
43	34.39633172664636	36.0	36.0	36.0	32.0	36.0
44	34.33314173837616	36.0	36.0	36.0	32.0	36.0
45	34.294850469684874	36.0	36.0	36.0	32.0	36.0
46	34.28258469606017	36.0	36.0	36.0	32.0	36.0
47	34.24333976500971	36.0	36.0	36.0	32.0	36.0
48	34.206469132881885	36.0	36.0	36.0	32.0	36.0
49	34.19556300578604	36.0	36.0	36.0	32.0	36.0
50	33.55954352347439	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	4.0
20	14.0
21	55.0
22	181.0
23	505.0
24	1260.0
25	2976.0
26	5948.0
27	11035.0
28	18269.0
29	28147.0
30	41947.0
31	60543.0
32	88733.0
33	145527.0
34	302821.0
35	915249.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63037389453865	18.028215526302493	11.89764139492988	26.443769184228977
2	16.12454295949705	20.298912959774277	36.574267734095606	27.00227634663307
3	18.11269610002372	24.061630775960055	28.8307463891105	28.99492673490573
4	12.352884643818198	15.914456239958206	35.76153758957526	35.97112152664833
5	14.505956077318114	36.77963992469271	33.236673369409864	15.477730628579314
6	34.861844995080745	35.51320744720502	16.405918385325894	13.219029172388344
7	30.57161250974147	30.402442067132203	20.560615814910534	18.465329608215793
8	28.436634434357472	32.95679687731023	18.927487161289687	19.679081527042612
9	27.589085436138365	13.723355690557293	18.074358562065168	40.613200311239176
10	15.78061525609385	26.849594071934717	31.605217860588446	25.76457281138299
11	37.67058727858769	20.774006663315294	22.220825817389674	19.334580240707336
12	24.7585193581873	23.559355969480322	28.622024808789963	23.060099863542412
13	29.75712954138433	19.236254559635192	25.031434607084353	25.975181291896128
14	23.762210328138707	19.1535815319711	25.096228721254594	31.987979418635597
15	25.412966011474087	27.01825355361473	22.00893825286359	25.559842182047593
16	26.10076706080363	25.543845555316658	23.358914348059923	24.996473035819786
17	24.330218529142147	25.839629476298903	24.912580950409556	24.91757104414939
18	25.17774953471853	24.878219775494376	25.667581109295096	24.276449580492
19	25.861392360223384	24.733384055716588	25.07887125242189	24.326352331638137
20	26.052931018656277	24.099637322904595	24.669312047077174	25.178119611361954
21	27.058074834156393	24.013008743137082	24.20891612699727	24.72000029570926
22	26.18927633694633	24.01685791275827	24.6953266790454	25.098539071250002
23	24.757871002122343	23.953659580890893	25.18449610492821	26.10397331205855
24	25.204332898597777	24.646548904053635	24.886628893753056	25.26248930359553
25	25.24718347300712	24.16819503852606	25.13937087963371	25.445250608833113
26	25.155424341876255	24.911760375125453	25.301684331020734	24.63113095197756
27	25.607497594160435	24.62698705097669	24.575851408448102	25.189663946414775
28	24.924668687001862	24.406852546540303	25.353153072119067	25.31532569433877
29	25.00921030420495	24.59594795900891	25.14640995279642	25.248431783989716
30	24.944891650022242	24.773991076698255	25.233586706473645	25.047530566805857
31	25.406181362265535	24.562797847335816	24.555158169064846	25.4758626213338
32	25.328093368145954	24.531563623190728	24.329551013266666	25.81079199539665
33	24.90909650987278	24.29430428487817	24.935834642516095	25.860764562732957
34	25.58091824419359	24.302673237376656	25.129885291865328	24.98652322656443
35	25.985525844813452	24.221137786638362	25.026784025569732	24.766552342978457
36	24.976634175283426	24.891796362139786	24.749044573429686	25.382524889147106
37	25.935783247359005	24.584159654106593	24.546024141812357	24.934032956722046
38	25.07741349588627	24.453731188797747	25.052214833078885	25.416640482237103
39	25.676444114771403	24.380204749140102	24.522889652695333	25.420461483393165
40	25.883788755666338	24.541240600253083	24.84397929498972	24.73099134909086
41	24.982873083387137	24.820352198406088	25.255363558850057	24.94141115935672
42	26.072505598560852	24.928766591608472	24.608963242021087	24.389764567809586
43	25.35497782158699	24.18629866929522	24.94276737309019	25.5159561360276
44	25.065703629501762	24.739680556530995	24.585109148729853	25.60950666523739
45	25.070709398081437	24.838451126883864	24.785900382764094	25.304939092270608
46	25.016864824504175	24.555615724731563	24.853607162637285	25.573912288126976
47	25.27017919124288	24.508527078294364	25.13051524721196	25.090778483250798
48	25.910232043790447	25.046867732267113	24.22829190757677	24.814608316365664
49	25.13300788806391	25.108981451591045	24.415171893751403	25.342838766593644
50	25.135426684154723	25.17251385070136	24.416970643844298	25.27508882129962
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	24.0
1	26.0
2	28.0
3	81.5
4	135.0
5	157.0
6	179.0
7	178.5
8	178.0
9	260.0
10	342.0
11	491.5
12	641.0
13	1343.0
14	2045.0
15	2688.0
16	3331.0
17	3586.0
18	3841.0
19	3849.0
20	3857.0
21	4199.5
22	4542.0
23	5303.5
24	6065.0
25	7117.5
26	8170.0
27	10160.0
28	12150.0
29	14618.5
30	17087.0
31	19817.0
32	22547.0
33	26264.0
34	29981.0
35	34748.0
36	39515.0
37	47834.5
38	56154.0
39	61484.5
40	66815.0
41	73913.5
42	81012.0
43	84701.5
44	88391.0
45	97570.0
46	106749.0
47	114206.5
48	121664.0
49	125195.0
50	128726.0
51	126630.5
52	124535.0
53	123404.5
54	122274.0
55	122459.0
56	122644.0
57	119214.0
58	115784.0
59	105475.0
60	95166.0
61	83826.0
62	72486.0
63	62548.0
64	52610.0
65	44391.5
66	36173.0
67	30902.0
68	25631.0
69	22841.0
70	20051.0
71	15638.5
72	11226.0
73	9591.5
74	7957.0
75	6161.5
76	4366.0
77	3834.0
78	3302.0
79	2677.5
80	2053.0
81	1637.5
82	1222.0
83	1013.0
84	804.0
85	575.0
86	346.0
87	244.5
88	143.0
89	117.0
90	91.0
91	76.0
92	61.0
93	47.0
94	33.0
95	37.5
96	42.0
97	30.5
98	19.0
99	23.5
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008501641186385545
2	6.160609555351845E-5
3	6.160609555351845E-5
4	0.0
5	0.0
6	1.8481828666055535E-4
7	6.160609555351845E-5
8	0.0
9	0.001663364579944998
10	0.0013553341021774058
11	0.0
12	6.160609555351845E-5
13	3.0803047776759224E-4
14	0.0
15	0.004928487644281476
16	4.312426688746291E-4
17	0.0
18	3.0803047776759224E-4
19	6.160609555351845E-5
20	5.54454859981666E-4
21	0.0
22	1.232121911070369E-4
23	6.776670510887029E-4
24	4.312426688746291E-4
25	0.0017865767710520352
26	0.004866881548727957
27	0.0050516998353885125
28	0.0032651230643364775
29	0.0020946072488196273
30	0.003080304777675922
31	0.006838276606440547
32	0.003080304777675922
33	0.004066002306532217
34	0.0032651230643364775
35	0.002895486491015367
36	0.007331125370868696
37	0.003511547446550552
38	0.007023094893101104
39	0.0036347596376575886
40	0.0020946072488196273
41	0.002464243822140738
42	0.001909788962159072
43	9.856975288562951E-4
44	0.001108909719963332
45	0.0014169401977309243
46	9.240914333027767E-4
47	0.0020946072488196273
48	0.001909788962159072
49	2.464243822140738E-4
50	8.008792421957398E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1623216.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.38910932582009
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.60144307809243	45.10866809975872
2	12.759794947405068	14.645465344233525
3	3.868883175687341	6.660952786150405
4	1.6110792735211517	3.6983361824267265
5	0.8242995626340495	2.3652908858615573
6	0.5364890143974993	1.847317601961573
7	0.36017948729379606	1.4469265981255042
8	0.24018704576812552	1.1027296502586166
9	0.17958028176472107	0.9275357180661439
>10	0.8899861361750219	9.025873975242506
>50	0.06266751767065785	2.4891733176597017
>100	0.05677569942848621	6.736221707612666
>500	0.0065840198733487275	2.5003827757197663
>1k	0.0020507602884200955	1.4451253569227107
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	6.160609555351845E-5	0.0
9	0.0	0.0	0.0	6.160609555351845E-5	0.0
10	0.0	0.0	0.0	6.160609555351845E-5	0.0
11	0.0	0.0	0.0	6.160609555351845E-5	0.0
12	0.0	0.0	0.0	6.160609555351845E-5	6.160609555351845E-5
13	0.0	0.0	0.0	6.160609555351845E-5	1.232121911070369E-4
14	0.0	0.0	0.0	6.160609555351845E-5	1.232121911070369E-4
15	0.0	0.0	0.0	6.160609555351845E-5	2.464243822140738E-4
16	0.0	0.0	0.0	1.232121911070369E-4	3.696365733211107E-4
17	0.0	0.0	0.0	1.232121911070369E-4	3.696365733211107E-4
18	0.0	0.0	0.0	1.8481828666055535E-4	4.312426688746291E-4
19	0.0	0.0	0.0	2.464243822140738E-4	4.312426688746291E-4
20	0.0	0.0	0.0	2.464243822140738E-4	4.312426688746291E-4
21	0.0	0.0	0.0	3.0803047776759224E-4	4.928487644281476E-4
22	0.0	0.0	0.0	4.312426688746291E-4	4.928487644281476E-4
23	0.0	0.0	0.0	5.54454859981666E-4	4.928487644281476E-4
24	0.0	0.0	0.0	0.001108909719963332	5.54454859981666E-4
25	0.0	0.0	0.0	0.0014169401977309243	5.54454859981666E-4
26	0.0	0.0	0.0	0.0016633645799449981	6.160609555351845E-4
27	0.0	0.0	0.0	0.0019713950577125903	6.160609555351845E-4
28	0.0	0.0	0.0	0.003634759637657588	6.160609555351845E-4
29	0.0	0.0	0.0	0.006591852224226474	6.160609555351845E-4
30	0.0	0.0	0.0	0.012013188632936097	6.160609555351845E-4
31	0.0	0.0	0.0	0.027969167381297375	6.776670510887029E-4
32	0.0	0.0	0.0	0.04460281318074735	7.392731466422214E-4
33	0.0	0.0	0.0	0.06025076145134104	7.392731466422214E-4
34	0.0	0.0	0.0	0.08051916688844861	9.240914333027767E-4
35	0.0	0.0	0.0	0.10275896738326877	9.240914333027767E-4
36	0.0	0.0	0.0	0.1343012883066702	9.240914333027767E-4
37	0.0	0.0	0.0	0.18192280016954	9.240914333027767E-4
38	0.0	0.0	0.0	0.24858059555844694	9.856975288562951E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	400	0.0	22.000154	10
ACCGTCG	385	0.0	21.7131	8
TACCGTC	420	0.0	21.47502	7
GTATCAA	2835	0.0	20.876213	1
ATTAGAC	130	1.6279955E-9	20.305958	3
ATACCGT	435	0.0	20.228157	6
CCGTCGT	455	0.0	18.373755	9
TAGGACG	185	2.1827873E-11	17.836315	4
GTCGTAG	485	0.0	17.690845	11
GGTATCA	1515	0.0	17.426939	1
CGTCTTA	190	3.274181E-11	17.368008	15
TATAGGA	230	0.0	17.21592	2
GTACTAA	90	2.2147984E-4	17.112286	1
CGCAATA	465	0.0	17.033426	36
GTATATG	210	9.094947E-12	16.763056	1
AAGACGG	595	0.0	16.637234	5
AACGCAG	3520	0.0	16.623579	6
CAAGACG	590	0.0	16.405378	4
CGCTAGA	565	0.0	16.3546	37
GTAATAC	175	3.6325218E-9	16.341461	3
>>END_MODULE