FastQCFastQC Report
Thu 2 Feb 2017
SRR4064183_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064183_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2109
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT170.8060692271218587No Hit
TATCAACGCAGAGTACTTTTTTTTT140.6638217164532955No Hit
GTACTTTTTTTTTTTTTTTTTTTTT70.33191085822664773No Hit
GGTATCAACGCAGAGTACTTTTTTT60.2844950213371266No Hit
CTCCACTCCTGGTGGTGCCCTTCCG50.2370791844476055No Hit
TTCGTATTGCGCCGCTAGAGGTGAA30.1422475106685633No Hit
ACCATACTCCCCCCGGAACCCAAAG30.1422475106685633No Hit
GTATCAACGCAGAGTACATGGGGAG30.1422475106685633No Hit
GTTTATGGTCGGAACTACGACGGTA30.1422475106685633No Hit
GCGCAAGACGGACCAGAGCGAAAGC30.1422475106685633No Hit
ATCAACGCAGAGTACTTTTTTTTTT30.1422475106685633No Hit
GAGTACTTTTTTTTTTTTTTTTTTT30.1422475106685633No Hit
CCACTCCTGGTGGTGCCCTTCCGTC30.1422475106685633No Hit
ATATTAAAGTTGCTGCAGTTAAAAA30.1422475106685633No Hit
GTCCAAATGTGCCCATCATCCTGGT30.1422475106685633No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30.1422475106685633No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers