##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064183_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2109
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.892366050260787	32.0	32.0	32.0	21.0	32.0
2	28.646277856804172	32.0	32.0	32.0	14.0	32.0
3	28.765291607396872	32.0	32.0	32.0	14.0	32.0
4	28.55523944997629	32.0	32.0	32.0	14.0	32.0
5	28.318160265528686	32.0	32.0	32.0	14.0	32.0
6	31.767662399241345	36.0	32.0	36.0	14.0	36.0
7	31.848269321953534	36.0	32.0	36.0	14.0	36.0
8	31.61071597913703	36.0	32.0	36.0	14.0	36.0
9	32.1683262209578	36.0	32.0	36.0	14.0	36.0
10	31.175912754860125	36.0	32.0	36.0	14.0	36.0
11	31.913229018492178	36.0	32.0	36.0	14.0	36.0
12	31.589853010905642	36.0	32.0	36.0	14.0	36.0
13	31.859649122807017	36.0	32.0	36.0	14.0	36.0
14	31.34234234234234	36.0	32.0	36.0	14.0	36.0
15	31.336178283546705	36.0	32.0	36.0	14.0	36.0
16	31.37932669511617	36.0	32.0	36.0	14.0	36.0
17	31.15220483641536	36.0	32.0	36.0	14.0	36.0
18	31.110478899952586	36.0	32.0	36.0	14.0	36.0
19	31.28117591275486	36.0	32.0	36.0	14.0	36.0
20	31.328117591275486	36.0	32.0	36.0	14.0	36.0
21	31.083926031294453	36.0	32.0	36.0	14.0	36.0
22	31.3072546230441	36.0	32.0	36.0	14.0	36.0
23	31.09815078236131	36.0	32.0	36.0	14.0	36.0
24	31.012328117591274	36.0	32.0	36.0	14.0	36.0
25	29.892366050260787	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	2.0
16	5.0
17	13.0
18	13.0
19	17.0
20	21.0
21	22.0
22	37.0
23	47.0
24	40.0
25	63.0
26	94.0
27	90.0
28	113.0
29	134.0
30	153.0
31	163.0
32	212.0
33	225.0
34	346.0
35	297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.65085388994307	20.49335863377609	11.527514231499051	26.328273244781787
2	16.4058795637743	19.25082977714557	39.82930298719772	24.513987671882408
3	17.78093883357041	25.177809388335703	28.87624466571835	28.165007112375534
4	12.233285917496444	16.216216216216218	34.6135609293504	36.93693693693694
5	13.229018492176387	36.79468942626837	33.61782835467046	16.35846372688478
6	31.10478899952584	37.69559032716928	16.548127074442863	14.65149359886202
7	28.449502133712663	31.199620673304885	21.47937411095306	18.8715030820294
8	27.32447817836812	32.63757115749525	18.548387096774192	21.48956356736243
9	27.798861480075903	12.7134724857685	18.12144212523719	41.36622390891841
10	13.757115749525617	26.85009487666034	33.15939278937382	26.23339658444023
11	36.8595825426945	21.537001897533205	23.197343453510435	18.40607210626186
12	26.505452821242294	22.33285917496444	27.78568041725936	23.376007586533902
13	27.59601706970128	19.630156472261735	26.458036984352773	26.31578947368421
14	20.588235294117645	18.785578747628083	28.51043643263757	32.1157495256167
15	25.901328273244783	25.66413662239089	22.81783681214421	25.616698292220114
16	25.889046941678522	25.841631104789	24.940730203888098	23.32859174964438
17	23.281175912754858	26.126126126126124	26.410621147463253	24.182076813655762
18	25.651967757230913	24.466571834992887	26.31578947368421	23.565670934091987
19	24.419155998103367	24.940730203888098	26.884779516358464	23.75533428165007
20	24.466571834992887	23.992413466097677	26.837363679468947	24.703651019440493
21	25.225225225225223	23.32859174964438	26.31578947368421	25.130393551446183
22	23.75533428165007	23.850165955429116	27.975343764817453	24.419155998103367
23	22.52252252252252	25.794215267899478	26.600284495021338	25.082977714556666
24	23.57685009487666	25.521821631878556	27.561669829222012	23.33965844402277
25	24.08724513987672	24.134660976766238	28.82882882882883	22.94926505452821
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	4.5
7	8.0
8	8.0
9	8.0
10	8.0
11	8.0
12	8.0
13	8.0
14	8.0
15	8.0
16	8.0
17	8.0
18	14.5
19	21.0
20	21.0
21	21.0
22	36.0
23	51.0
24	51.0
25	51.0
26	67.5
27	84.0
28	84.0
29	84.0
30	95.5
31	107.0
32	107.0
33	107.0
34	123.5
35	140.0
36	140.0
37	140.0
38	164.0
39	188.0
40	188.0
41	188.0
42	202.0
43	216.0
44	216.0
45	216.0
46	233.0
47	250.0
48	250.0
49	250.0
50	245.5
51	241.0
52	241.0
53	241.0
54	242.5
55	244.0
56	244.0
57	244.0
58	207.5
59	171.0
60	171.0
61	171.0
62	165.0
63	159.0
64	159.0
65	159.0
66	126.0
67	93.0
68	93.0
69	93.0
70	75.0
71	57.0
72	57.0
73	57.0
74	43.5
75	30.0
76	30.0
77	30.0
78	21.0
79	12.0
80	12.0
81	12.0
82	10.0
83	8.0
84	8.0
85	8.0
86	6.0
87	4.0
88	4.0
89	4.0
90	5.0
91	6.0
92	6.0
93	6.0
94	3.0
95	0.0
96	0.0
97	0.0
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0474158368895211
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0474158368895211
9	0.0474158368895211
10	0.0474158368895211
11	0.0474158368895211
12	0.0
13	0.0
14	0.0474158368895211
15	0.0474158368895211
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0474158368895211
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2109.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.35751541014699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.53266331658291	91.08582266477003
2	2.6633165829145726	5.026078710289237
3	0.5527638190954774	1.5647226173541962
4	0.0	0.0
5	0.05025125628140704	0.2370791844476055
6	0.05025125628140704	0.2844950213371266
7	0.05025125628140704	0.33191085822664773
8	0.0	0.0
9	0.0	0.0
>10	0.10050251256281408	1.469890943575154
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	17	0.8060692271218587	No Hit
TATCAACGCAGAGTACTTTTTTTTT	14	0.6638217164532955	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	7	0.33191085822664773	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6	0.2844950213371266	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	5	0.2370791844476055	No Hit
TTCGTATTGCGCCGCTAGAGGTGAA	3	0.1422475106685633	No Hit
ACCATACTCCCCCCGGAACCCAAAG	3	0.1422475106685633	No Hit
GTATCAACGCAGAGTACATGGGGAG	3	0.1422475106685633	No Hit
GTTTATGGTCGGAACTACGACGGTA	3	0.1422475106685633	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	3	0.1422475106685633	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	3	0.1422475106685633	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3	0.1422475106685633	No Hit
CCACTCCTGGTGGTGCCCTTCCGTC	3	0.1422475106685633	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	3	0.1422475106685633	No Hit
GTCCAAATGTGCCCATCATCCTGGT	3	0.1422475106685633	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3	0.1422475106685633	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE