FastQCFastQC Report
Thu 2 Feb 2017
SRR4064182_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064182_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668123
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31900.4774569951939987No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23910.3578682368366304No Hit
TATCAACGCAGAGTACTTTTTTTTT23790.3560721603656812No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14900.22301282847619375No Hit
GTACATGGGGTGGTATCAACGCAAA13520.20235794906027782No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12560.18798933729268413No Hit
GGTATCAACGCAGAGTACTTTTTTT11750.17586582111377697No Hit
GTATCAACGCAGAGTACATGGGGTG8710.1303652171830636No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG7660.114649548062258No Hit
GATTAAGAGGGACGGCCGGGGGCAT7450.11150641423809687No Hit
GAATAGGACCGCGGTTCTATTTTGT6840.10237635884410505No Hit
CTATTGGAGCTGGAATTACCGCGGC6820.10207701276561351No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTAG307.7215396E-418.9981276
GTATATG402.766549E-416.6183821
GTCTAAA350.002174231216.2792321
GTATAGA456.7756063E-414.7718951
TAGAACC1300.014.6150384
AACCGCG851.7078492E-814.5312457
ATTCGTG400.005270515614.25179811
CGGTCGG602.5608826E-514.25179810
TGCACCG400.00527838114.2485955
GTTCTAA400.00528888414.2443271
ACAGCCC954.8676156E-914.0028168
TCGGCGT551.9566646E-413.81889113
ACTCTAA1257.2759576E-1213.68172610
CGAGCCG1153.7471182E-1013.21905915
AGAACCG957.3714546E-812.998725
GAACCGC957.3714546E-812.998726
CGGTCCA1251.0186341E-1012.92163110
CGCGGTC1051.9821528E-812.66826410
TAGCGTA751.4736797E-512.6682647
CGGCGTC604.084826E-412.66731614
Produced by FastQC (version 0.11.5)