##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064182_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	668123
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.130633131923314	32.0	32.0	32.0	32.0	32.0
2	30.69663669713511	32.0	32.0	32.0	32.0	32.0
3	30.712163778226465	32.0	32.0	32.0	32.0	32.0
4	30.7422405754629	32.0	32.0	32.0	32.0	32.0
5	30.593097378776065	32.0	32.0	32.0	32.0	32.0
6	34.247512808270336	36.0	36.0	36.0	32.0	36.0
7	34.14584290617147	36.0	36.0	36.0	32.0	36.0
8	34.101879444353806	36.0	36.0	36.0	32.0	36.0
9	34.269192948005085	36.0	36.0	36.0	32.0	36.0
10	33.8968288773175	36.0	36.0	36.0	32.0	36.0
11	34.2814736208752	36.0	36.0	36.0	32.0	36.0
12	34.035399170512015	36.0	36.0	36.0	32.0	36.0
13	34.16354174306228	36.0	36.0	36.0	32.0	36.0
14	34.04221079052809	36.0	36.0	36.0	32.0	36.0
15	33.954695467750696	36.0	36.0	36.0	32.0	36.0
16	33.95141014453925	36.0	36.0	36.0	32.0	36.0
17	33.85408824423048	36.0	36.0	36.0	32.0	36.0
18	33.85357785916665	36.0	36.0	36.0	32.0	36.0
19	33.87927522327475	36.0	36.0	36.0	32.0	36.0
20	33.84217127684573	36.0	36.0	36.0	32.0	36.0
21	33.82857497796064	36.0	36.0	36.0	32.0	36.0
22	33.79901604944	36.0	36.0	36.0	32.0	36.0
23	33.75854296289755	36.0	36.0	36.0	32.0	36.0
24	33.74508585993896	36.0	36.0	36.0	32.0	36.0
25	33.33805451990128	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	6.0
5	9.0
6	28.0
7	11.0
8	28.0
9	45.0
10	49.0
11	31.0
12	42.0
13	32.0
14	95.0
15	170.0
16	212.0
17	316.0
18	377.0
19	544.0
20	828.0
21	1193.0
22	1803.0
23	3021.0
24	4093.0
25	6021.0
26	8526.0
27	11351.0
28	15301.0
29	20829.0
30	27513.0
31	37436.0
32	51856.0
33	72384.0
34	145509.0
35	258462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.340029257234576	17.358643686112096	11.773716103008224	25.5276109536451
2	16.29855259021988	20.261491715188072	38.07121794967744	25.36873774491461
3	18.845258675527415	24.71894808528472	28.699699901962983	27.736093337224876
4	12.489129507418879	15.459524347658185	35.85189487092366	36.19945127399928
5	14.362418719388796	37.02354293652029	33.13659333948053	15.477445004610392
6	33.251501783578505	35.62381836988559	17.29090666862264	13.833773177913267
7	29.607622982724035	30.676666966076827	21.001526991826104	18.714183059373035
8	28.152563359699833	32.44741298381627	19.921454484475806	19.478569172008093
9	28.060124345628136	13.689549217149635	18.863579189477342	39.386747247744886
10	16.437960227094038	26.297022348485417	31.21315000876088	26.051867415659665
11	36.849726996906064	20.88960371871247	23.153447805941674	19.107221478439797
12	25.486560520715233	23.592737424310727	28.892896029186893	22.027806025787147
13	29.917523951302137	19.453534398967214	25.56353141862698	25.06541023110367
14	23.963809335525983	19.74206438355754	25.60230407706617	30.69182220385031
15	24.861094054675775	27.529585843125393	23.027086284928664	24.582233817270165
16	25.05432983715277	25.339046640536424	24.89167731296832	24.714946209342486
17	23.264705838304298	25.853999709455138	25.834380443035982	25.046914009204578
18	24.239110734309257	24.61388211927077	27.47844165804133	23.668565488378643
19	25.53093971724156	24.280559645436067	26.18818309949868	24.000317537823697
20	26.14904134212103	23.77336728579988	25.590173756740565	24.487417615338526
21	27.106168948855213	23.420916250520495	24.851340704637572	24.621574095986723
22	26.073801124916677	24.186008193467696	25.310026138210468	24.43016454340516
23	24.18863413713454	24.02865283157923	26.21386564566238	25.56884738562385
24	24.60924101010918	24.769963346919088	26.171078667068098	24.44971697590363
25	24.48897363348097	24.460814197149006	26.342403767373103	24.70780840199692
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	405.0
1	405.0
2	553.0
3	701.0
4	701.0
5	701.0
6	1779.0
7	2857.0
8	2857.0
9	2857.0
10	2640.5
11	2424.0
12	2424.0
13	2424.0
14	2054.5
15	1685.0
16	1685.0
17	1685.0
18	3011.0
19	4337.0
20	4337.0
21	4337.0
22	7112.0
23	9887.0
24	9887.0
25	9887.0
26	15376.5
27	20866.0
28	20866.0
29	20866.0
30	26339.0
31	31812.0
32	31812.0
33	31812.0
34	37268.0
35	42724.0
36	42724.0
37	42724.0
38	47777.0
39	52830.0
40	52830.0
41	52830.0
42	60875.5
43	68921.0
44	68921.0
45	68921.0
46	77951.0
47	86981.0
48	86981.0
49	86981.0
50	88995.5
51	91010.0
52	91010.0
53	91010.0
54	81984.0
55	72958.0
56	72958.0
57	72958.0
58	67586.0
59	62214.0
60	62214.0
61	62214.0
62	54983.0
63	47752.0
64	47752.0
65	47752.0
66	39859.5
67	31967.0
68	31967.0
69	31967.0
70	24324.0
71	16681.0
72	16681.0
73	16681.0
74	12878.0
75	9075.0
76	9075.0
77	9075.0
78	7306.5
79	5538.0
80	5538.0
81	5538.0
82	3838.0
83	2138.0
84	2138.0
85	2138.0
86	1683.5
87	1229.0
88	1229.0
89	1229.0
90	873.0
91	517.0
92	517.0
93	517.0
94	362.5
95	208.0
96	208.0
97	208.0
98	307.0
99	406.0
100	406.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03801695196842498
2	0.0049392102951103314
3	0.001197384313966141
4	0.004490191177373029
5	0.01002809362946643
6	0.012572535296644479
7	0.021403244612144767
8	0.03442479902652655
9	0.045949623048450655
10	0.05717510099188323
11	0.054630659324705184
12	0.063760714718697
13	0.06196463824774779
14	0.06540711815040046
15	0.06061758089453589
16	0.0661554833466293
17	0.061665292169256265
18	0.07723128825081609
19	0.07274109707344306
20	0.07827899952553646
21	0.07319011619118038
22	0.07753063432930762
23	0.08157180638894336
24	0.07663259609383302
25	0.07423782746590074
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	668123.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.06637516958536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.2898805980715	37.69491408496334
2	14.076198945646611	14.094885147489302
3	4.5597891370334045	6.848763408867428
4	1.9755359414578744	3.9563169442411983
5	1.0906251597701975	2.7301824209221843
6	0.617273519799163	1.8542788574709226
7	0.41953774417084105	1.4703313868221195
8	0.3059925589029123	1.2255950602507724
9	0.25159692260000577	1.1336891326564518
>10	1.1839733322680333	10.705657110749565
>50	0.11494815094969779	4.001779143801153
>100	0.10594434616076628	10.24531545641934
>500	0.006602763783389273	2.0498745062032775
>1k	0.0021008793856238596	1.9884173391429387
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3190	0.4774569951939987	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2391	0.3578682368366304	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2379	0.3560721603656812	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1490	0.22301282847619375	No Hit
GTACATGGGGTGGTATCAACGCAAA	1352	0.20235794906027782	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1256	0.18798933729268413	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1175	0.17586582111377697	No Hit
GTATCAACGCAGAGTACATGGGGTG	871	0.1303652171830636	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	766	0.114649548062258	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	745	0.11150641423809687	No Hit
GAATAGGACCGCGGTTCTATTTTGT	684	0.10237635884410505	No Hit
CTATTGGAGCTGGAATTACCGCGGC	682	0.10207701276561351	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.4967303924576762E-4
11	0.0	0.0	0.0	0.0	1.4967303924576762E-4
12	0.0	0.0	0.0	0.0	1.4967303924576762E-4
13	0.0	0.0	0.0	0.0	1.4967303924576762E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACTAG	30	7.7215396E-4	18.998127	6
GTATATG	40	2.766549E-4	16.618382	1
GTCTAAA	35	0.0021742312	16.279232	1
GTATAGA	45	6.7756063E-4	14.771895	1
TAGAACC	130	0.0	14.615038	4
AACCGCG	85	1.7078492E-8	14.531245	7
ATTCGTG	40	0.0052705156	14.251798	11
CGGTCGG	60	2.5608826E-5	14.251798	10
TGCACCG	40	0.005278381	14.248595	5
GTTCTAA	40	0.005288884	14.244327	1
ACAGCCC	95	4.8676156E-9	14.002816	8
TCGGCGT	55	1.9566646E-4	13.818891	13
ACTCTAA	125	7.2759576E-12	13.681726	10
CGAGCCG	115	3.7471182E-10	13.219059	15
AGAACCG	95	7.3714546E-8	12.99872	5
GAACCGC	95	7.3714546E-8	12.99872	6
CGGTCCA	125	1.0186341E-10	12.921631	10
CGCGGTC	105	1.9821528E-8	12.668264	10
TAGCGTA	75	1.4736797E-5	12.668264	7
CGGCGTC	60	4.084826E-4	12.667316	14
>>END_MODULE