FastQCFastQC Report
Thu 2 Feb 2017
SRR4064182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668123
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10110.15131944267747108No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA9060.13560377355666547No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8460.12662339120191943No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA6900.10327439707957967No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6760.10117897453013891No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT6720.10058028237315583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG250.00235082535.20371235
GTACTAA300.005746541529.329841
TGTAGGA4200.023.0448762
TTGAATC500.00257878222.00067541
CCGCGTT500.00257990321.9990279
GCATATA500.002581024521.997381
GTAGGAC4100.021.460863
AATCGCT1351.1277734E-1021.18583315
CGAACGT1259.949872E-1021.1174854
CTGTAGG4650.020.8147261
GGTATAT654.932946E-420.3052751
CCCTTAC654.932946E-420.3052751
TACTAAA654.932946E-420.3052752
TAGGACG3700.020.2138084
GACGGTA2000.019.7976428
ACGACGG2000.019.7976426
AAATCGC1452.9467628E-1019.72474114
CAAATCG1452.9467628E-1019.72474113
GTATATG901.0018895E-519.5532281
TATATGA808.989118E-519.247712
Produced by FastQC (version 0.11.5)