##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064182_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	668123
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.125617588378187	32.0	32.0	32.0	32.0	32.0
2	31.31271038416579	32.0	32.0	32.0	32.0	32.0
3	31.396584161898332	32.0	32.0	32.0	32.0	32.0
4	31.514584889309305	32.0	32.0	32.0	32.0	32.0
5	31.417254008618173	32.0	32.0	32.0	32.0	32.0
6	35.00287970927509	36.0	36.0	36.0	36.0	36.0
7	35.02277275292124	36.0	36.0	36.0	36.0	36.0
8	34.96985734662629	36.0	36.0	36.0	36.0	36.0
9	35.104798068619104	36.0	36.0	36.0	36.0	36.0
10	34.92985573015747	36.0	36.0	36.0	32.0	36.0
11	35.10767478443341	36.0	36.0	36.0	36.0	36.0
12	34.99988924195096	36.0	36.0	36.0	36.0	36.0
13	35.046069361479844	36.0	36.0	36.0	36.0	36.0
14	34.99425105856257	36.0	36.0	36.0	36.0	36.0
15	34.95268984902481	36.0	36.0	36.0	36.0	36.0
16	34.973016944484776	36.0	36.0	36.0	36.0	36.0
17	34.92345421426893	36.0	36.0	36.0	32.0	36.0
18	34.91120497273705	36.0	36.0	36.0	32.0	36.0
19	34.91403229644841	36.0	36.0	36.0	32.0	36.0
20	34.90713985299114	36.0	36.0	36.0	32.0	36.0
21	34.90268109315201	36.0	36.0	36.0	32.0	36.0
22	34.89188368010082	36.0	36.0	36.0	32.0	36.0
23	34.842403270056565	36.0	36.0	36.0	32.0	36.0
24	34.80242709800441	36.0	36.0	36.0	32.0	36.0
25	34.77481990591553	36.0	36.0	36.0	32.0	36.0
26	34.71075535492716	36.0	36.0	36.0	32.0	36.0
27	34.715116827290785	36.0	36.0	36.0	32.0	36.0
28	34.68198071313216	36.0	36.0	36.0	32.0	36.0
29	34.63937179231968	36.0	36.0	36.0	32.0	36.0
30	34.61273897171628	36.0	36.0	36.0	32.0	36.0
31	34.59421543637923	36.0	36.0	36.0	32.0	36.0
32	34.55054234025771	36.0	36.0	36.0	32.0	36.0
33	34.50742752457257	36.0	36.0	36.0	32.0	36.0
34	34.468526004942206	36.0	36.0	36.0	32.0	36.0
35	34.41862950384884	36.0	36.0	36.0	32.0	36.0
36	34.381055584076584	36.0	36.0	36.0	32.0	36.0
37	34.36969091020666	36.0	36.0	36.0	32.0	36.0
38	34.30726378226764	36.0	36.0	36.0	32.0	36.0
39	34.30187106266361	36.0	36.0	36.0	32.0	36.0
40	34.265307435906266	36.0	36.0	36.0	32.0	36.0
41	34.24717454720164	36.0	36.0	36.0	32.0	36.0
42	34.15701300509038	36.0	36.0	36.0	32.0	36.0
43	34.142198367665834	36.0	36.0	36.0	32.0	36.0
44	34.04687759589177	36.0	36.0	36.0	32.0	36.0
45	33.98859790787026	36.0	36.0	36.0	32.0	36.0
46	33.93266060291294	36.0	36.0	36.0	32.0	36.0
47	33.88038430049557	36.0	36.0	36.0	32.0	36.0
48	33.7940933031792	36.0	36.0	36.0	32.0	36.0
49	33.7638309113741	36.0	36.0	36.0	27.0	36.0
50	33.14506011617621	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	9.0
21	32.0
22	94.0
23	265.0
24	708.0
25	1495.0
26	3052.0
27	5580.0
28	9392.0
29	13850.0
30	19565.0
31	27382.0
32	39647.0
33	62279.0
34	123519.0
35	361251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.42777081733554	18.480797412212358	12.29648190808923	25.79494986236287
2	15.598199131296381	21.161404653641103	37.37970011464972	25.8606961004128
3	17.989828205028424	25.448645606640703	29.097679765072847	27.463846423258026
4	12.21781019363201	16.161694777757987	36.0408188312631	35.57967619734689
5	14.051903616549646	37.332347486914834	32.85173538405354	15.764013512481984
6	33.15482248697839	36.2123570616057	17.01969706040831	13.613123391007603
7	29.34773986227087	31.05760466261452	21.012747652752562	18.58190782236205
8	28.008208081757523	32.72336489443545	19.61842298262892	19.650004041178107
9	28.10440103215609	13.602727670045322	18.675499464164904	39.61737183363368
10	16.33895362325348	26.56324135814942	31.156911609528297	25.9408934090688
11	37.00396483880962	20.96440326107618	23.00175267128957	19.029879228824633
12	25.32411745160315	23.74835733593565	28.925405839053347	22.00211937340785
13	29.908893475563485	19.547715302214126	25.560566306301723	24.982824915920666
14	23.944351314135183	19.797192121175836	25.549751541639225	30.708705023049752
15	24.971336368834326	27.63982313866013	22.931952689292228	24.45688780321332
16	25.03633345656524	25.53145631678284	24.731895760772442	24.70031446587948
17	23.3648594644998	25.845390743919904	25.752743132626776	25.037006658953516
18	24.242465062466227	24.394383652063027	27.71728474333242	23.645866542138325
19	25.756784304686413	24.0261149518876	26.40890973668022	23.808191006745762
20	26.221975426533294	23.49641306413977	25.958998322155185	24.322613187171747
21	27.152485395653198	23.36949932871642	25.100767373672213	24.37724790195817
22	26.14728126407862	23.944842491577152	25.79210714194841	24.115769102395817
23	24.15059660298511	23.85034934053368	26.42116039729628	25.57789365918493
24	24.715994982952218	24.507051107432474	26.408350570704155	24.368603338911157
25	24.767253198584662	24.047160039993535	26.568009962461158	24.61757679896065
26	24.091483995539555	25.17800612188386	26.90111436247839	23.82939552009819
27	24.765748019710095	24.69509822118177	25.928326378120115	24.61082738098802
28	24.01697964657668	24.676018644083015	26.61721254793487	24.689789161405432
29	24.15480846516181	24.46359318847543	26.49127454898421	24.890323797378546
30	24.018781675742183	24.887516502070056	26.582477420977163	24.5112244012106
31	24.867866631740448	24.49260936272125	25.688882236275866	24.950641769262432
32	24.256094932464794	25.13905187278475	25.459514800268224	25.14533839448223
33	23.9895104267077	24.622357399879057	26.286050424193075	25.10208174922017
34	24.806092482884353	24.63785469883341	26.41512822892313	24.14092458935911
35	25.23686502959129	24.616750136955133	26.235065903110605	23.911318930342972
36	24.161219489195204	25.415704336137875	25.80263230766237	24.62044386700455
37	25.06784133584193	25.241916291350286	25.356569271504544	24.33367310130324
38	24.473257213337565	25.30221474460684	25.770580240334816	24.453947801720783
39	25.19016671206919	24.760888372663892	25.339845352029194	24.70909956323773
40	25.46239127799157	25.062901977689066	25.85544444494337	23.619262299375997
41	24.316791847902554	25.25168463309275	26.432499423737237	23.99902409526746
42	25.426915149818818	25.882530792223402	25.560576374742368	23.129977683215415
43	24.35388343483903	24.96186988012399	25.964104552115153	24.720142132921822
44	24.02313990211193	25.713430423133914	25.1256529613387	25.137776713415455
45	24.28791666043017	25.95156486207361	25.091227492478787	24.669290985017437
46	24.234151306287092	25.319742858639604	25.241911946296668	25.204193888776633
47	24.72680192484714	25.262346487453318	25.32072054542325	24.69013104227629
48	25.147132781722608	25.78296193407014	24.335133452276892	24.73477183193036
49	24.63109116789075	25.933553753148765	24.547273764104897	24.888081314855587
50	24.58068282169104	26.48935055604616	24.29046115160677	24.63950547065603
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	212.0
1	126.0
2	40.0
3	140.5
4	241.0
5	274.0
6	307.0
7	268.0
8	229.0
9	327.0
10	425.0
11	669.5
12	914.0
13	1573.0
14	2232.0
15	2952.5
16	3673.0
17	3921.0
18	4169.0
19	4133.5
20	4098.0
21	4171.5
22	4245.0
23	4365.5
24	4486.0
25	4381.0
26	4276.0
27	5091.0
28	5906.0
29	7040.5
30	8175.0
31	9891.5
32	11608.0
33	14109.0
34	16610.0
35	19245.0
36	21880.0
37	24179.0
38	26478.0
39	27653.0
40	28828.0
41	30730.5
42	32633.0
43	33145.0
44	33657.0
45	36785.0
46	39913.0
47	42658.5
48	45404.0
49	47125.0
50	48846.0
51	47142.5
52	45439.0
53	45429.0
54	45419.0
55	45898.5
56	46378.0
57	45255.5
58	44133.0
59	40334.5
60	36536.0
61	32743.0
62	28950.0
63	25109.5
64	21269.0
65	18372.5
66	15476.0
67	13353.5
68	11231.0
69	10141.0
70	9051.0
71	7125.5
72	5200.0
73	4396.5
74	3593.0
75	2794.5
76	1996.0
77	1749.5
78	1503.0
79	1293.5
80	1084.0
81	854.5
82	625.0
83	510.0
84	395.0
85	283.0
86	171.0
87	126.5
88	82.0
89	63.5
90	45.0
91	27.5
92	10.0
93	14.5
94	19.0
95	16.5
96	14.0
97	13.0
98	12.0
99	9.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009279728433237593
2	1.4967303924576762E-4
3	1.4967303924576762E-4
4	0.0
5	0.0
6	4.4901911773730286E-4
7	0.0
8	1.4967303924576762E-4
9	0.0010477112747203734
10	0.001197384313966141
11	0.0
12	1.4967303924576762E-4
13	5.986921569830705E-4
14	4.4901911773730286E-4
15	0.004340518138127261
16	8.980382354746057E-4
17	0.0
18	2.9934607849153524E-4
19	0.0
20	5.986921569830705E-4
21	0.0
22	0.0
23	0.0010477112747203734
24	1.4967303924576762E-4
25	0.0022450955886865144
26	0.004190845098881494
27	0.005837248530584937
28	0.004340518138127261
29	0.002993460784915352
30	0.0037418259811441905
31	0.007184305883796846
32	0.004041172059635726
33	0.004639864216618796
34	0.0031431338241611198
35	0.0031431338241611198
36	0.005986921569830704
37	0.002993460784915352
38	0.00823201715851722
39	0.0037418259811441905
40	0.002993460784915352
41	0.0037418259811441905
42	0.002394768627932282
43	0.002394768627932282
44	0.001945749510194979
45	0.001945749510194979
46	0.001197384313966141
47	0.002694114706423817
48	0.0028437877456695846
49	5.986921569830705E-4
50	0.001945749510194979
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	668123.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.028351555390884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.94581596851229	42.34201411831704
2	13.402424117881296	14.75026612106443
3	4.3072459335734665	7.110619304046255
4	1.8225271302904806	4.011626545794491
5	0.9377662527412348	2.5801865516313107
6	0.5691677366984003	1.8792217385415435
7	0.37111624005756483	1.4295340448061762
8	0.2737002692444627	1.204901970943155
9	0.19906363975890068	0.9858729555493627
>10	0.9941855812883948	9.807901022612135
>50	0.08999813547153011	3.513678987043152
>100	0.0812444382427283	8.366319051596918
>500	0.00547100594225351	1.8656715364670882
>1k	2.735502971126755E-4	0.15218605158691514
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1011	0.15131944267747108	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	906	0.13560377355666547	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	846	0.12662339120191943	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	690	0.10327439707957967	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	676	0.10117897453013891	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	672	0.10058028237315583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.4967303924576762E-4
13	0.0	0.0	0.0	0.0	1.4967303924576762E-4
14	0.0	0.0	0.0	0.0	1.4967303924576762E-4
15	0.0	0.0	0.0	0.0	1.4967303924576762E-4
16	0.0	0.0	0.0	1.4967303924576762E-4	4.4901911773730286E-4
17	0.0	0.0	0.0	1.4967303924576762E-4	4.4901911773730286E-4
18	0.0	0.0	0.0	1.4967303924576762E-4	4.4901911773730286E-4
19	0.0	0.0	0.0	1.4967303924576762E-4	4.4901911773730286E-4
20	0.0	0.0	0.0	1.4967303924576762E-4	4.4901911773730286E-4
21	0.0	0.0	0.0	4.4901911773730286E-4	4.4901911773730286E-4
22	0.0	0.0	0.0	4.4901911773730286E-4	4.4901911773730286E-4
23	0.0	0.0	0.0	0.0025444416671780495	4.4901911773730286E-4
24	0.0	0.0	0.0	0.005687575491339169	4.4901911773730286E-4
25	0.0	0.0	0.0	0.006884959805305311	4.4901911773730286E-4
26	0.0	0.0	0.0	0.008082344119271452	7.48365196228838E-4
27	0.0	0.0	0.0	0.009728747550974895	7.48365196228838E-4
28	0.0	0.0	0.0	0.015266650003068297	7.48365196228838E-4
29	0.0	0.0	0.0	0.023498667161585517	0.0010477112747203734
30	0.0	0.0	0.0	0.04086073971409456	0.0010477112747203734
31	0.0	0.0	0.0	0.0730404431519346	0.0010477112747203734
32	0.0	0.0	0.0	0.11255412551281725	0.0010477112747203734
33	0.0	0.0	0.0	0.1469789245393438	0.0010477112747203734
34	0.0	0.0	0.0	0.1821520887620992	0.0010477112747203734
35	0.0	0.0	0.0	0.22391086671166835	0.0010477112747203734
36	0.0	0.0	0.0	0.27435068093749204	0.0010477112747203734
37	0.0	0.0	0.0	0.35562314124794386	0.0010477112747203734
38	0.0	0.0	0.0	0.46952432411397305	0.001197384313966141
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGG	25	0.002350825	35.203712	35
GTACTAA	30	0.0057465415	29.32984	1
TGTAGGA	420	0.0	23.044876	2
TTGAATC	50	0.002578782	22.000675	41
CCGCGTT	50	0.002579903	21.999027	9
GCATATA	50	0.0025810245	21.99738	1
GTAGGAC	410	0.0	21.46086	3
AATCGCT	135	1.1277734E-10	21.185833	15
CGAACGT	125	9.949872E-10	21.117485	4
CTGTAGG	465	0.0	20.814726	1
GGTATAT	65	4.932946E-4	20.305275	1
CCCTTAC	65	4.932946E-4	20.305275	1
TACTAAA	65	4.932946E-4	20.305275	2
TAGGACG	370	0.0	20.213808	4
GACGGTA	200	0.0	19.797642	8
ACGACGG	200	0.0	19.797642	6
AAATCGC	145	2.9467628E-10	19.724741	14
CAAATCG	145	2.9467628E-10	19.724741	13
GTATATG	90	1.0018895E-5	19.553228	1
TATATGA	80	8.989118E-5	19.24771	2
>>END_MODULE