FastQCFastQC Report
Thu 2 Feb 2017
SRR4064181_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064181_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510621
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT118600.7851075815839976No Hit
GTCCTACAGTGGACATTTCTAAATT109660.7259266222301954No Hit
CTGTAGGACGTGGAATATGGCAAGA107120.7091123451878399No Hit
CTTTAGGACGTGAAATATGGCGAGG96130.6363608079061526No Hit
GTCCTACAGTGTGCATTTCTCATTT66750.44187125692016727No Hit
CTGTAGGACCTGGAATATGGCGAGA53640.35508575612281307No Hit
CTGAAGGACCTGGAATATGGCGAGA51060.3380066873160111No Hit
GTATCAACGCAGAGTACTTTTTTTT50450.33396861290820135No Hit
ATTTAGAAATGTCCACTGTAGGACG48190.3190078782169717No Hit
GTCCTTCAGTGTGCATTTCTCATTT42250.2796863011966602No Hit
TTTCTAAATTTTCCACCTTTTTCAG37910.2509563947542104No Hit
TATCAACGCAGAGTACTTTTTTTTT35650.2359956600629807No Hit
GAATATGGCAAGAAAACTGAAAATC30770.20369106480050259No Hit
GGAATATGGCGAGAAAACTGAAAAT27840.18429506805479337No Hit
ATTCCAGGTCCTTCAGTGTGCATTT24470.16198636190017218No Hit
GCCATATTCCACGTCCTACAGTGGA24290.16079479896016274No Hit
CTGTAGGACATGGAATATGGCAAGA23330.15443979661344573No Hit
GTCCACTGTAGGACGTGGAATATGG21320.14113401045000698No Hit
CCATATTCCAGGTCCTTCAGTGTGC21230.14053822898000226No Hit
GAAATATGGCGAGGAAAACTGAAAA20940.13861848868776483No Hit
ACAGTGGACATTTCTAAATTTTCCA20830.13789031133553684No Hit
GACCTGGAATATGGCGAGAAAACTG19870.13153530898881982No Hit
ACCTGGAATATGGCGAGAAAACTGA19620.12988036046102894No Hit
TTCCAGGTCCTTCAGTGTGCATTTC19540.12935077693213584No Hit
GGTATCAACGCAGAGTACTTTTTTT19250.12743103663989844No Hit
CCATATTTCACGTCCTAAAGTGTGT19080.12630567164100062No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18670.12359155605542356No Hit
ATACACACTTTAGGACGTGAAATAT18610.12319436840875375No Hit
CCTAAAGTGTGTATTTCTCATTTTC18400.12180421164540942No Hit
CCACTGTAGGACGTGGAATATGGCA17920.1186267104720509No Hit
CACTTTAGGACGTGAAATATGGCGA17920.1186267104720509No Hit
GAAATACACACTTTAGGACGTGAAA17130.1133970731242317No Hit
GTGTATTTCTCATTTTCCGTGATTT16640.11015337400976154No Hit
ATTCCACGTCCTACAGTGGACATTT16060.10631389342528669No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15990.10585050783750523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1400.014.9304219
TAGGACC18950.014.3858314
CTCGAAC602.5651696E-514.25176418
TGACGAG400.005274677614.25176318
GACGTTA602.5683767E-514.2498757
TCCAACG2200.013.81989218
AAAGGCG604.0967838E-412.6648785
TAGAAAT15450.012.4804394
AATGTCC15550.012.4022318
GTATCAA40650.012.360291
CCACCTT11350.012.30548813
AAATGTC15100.012.2681057
TCGAACT701.0896008E-412.21579719
ATTTAGA14900.012.1115821
GTCGGGA550.003065533212.09240512
GCGTGCA550.003067054612.0916049
GTTAGAC550.003075434412.0872013
ACCTTTT11650.012.07096315
ATAGGAC4250.012.0668863
GAAATGT15200.012.0615966
Produced by FastQC (version 0.11.5)