##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064181_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1510621
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.335025794027754	32.0	32.0	32.0	32.0	32.0
2	31.11364399144458	32.0	32.0	32.0	32.0	32.0
3	31.095075468962765	32.0	32.0	32.0	32.0	32.0
4	31.122846167238507	32.0	32.0	32.0	32.0	32.0
5	31.12685246663458	32.0	32.0	32.0	32.0	32.0
6	34.721004805308546	36.0	36.0	36.0	32.0	36.0
7	34.60253233603928	36.0	36.0	36.0	32.0	36.0
8	34.49721008777185	36.0	36.0	36.0	32.0	36.0
9	34.68181165229399	36.0	36.0	36.0	32.0	36.0
10	34.4473815735383	36.0	36.0	36.0	32.0	36.0
11	34.63380225748219	36.0	36.0	36.0	32.0	36.0
12	34.4844815476549	36.0	36.0	36.0	32.0	36.0
13	34.52805568041223	36.0	36.0	36.0	32.0	36.0
14	34.50788318181728	36.0	36.0	36.0	32.0	36.0
15	34.49044598214906	36.0	36.0	36.0	32.0	36.0
16	34.569964272971184	36.0	36.0	36.0	32.0	36.0
17	34.43258434776162	36.0	36.0	36.0	32.0	36.0
18	34.50330096033353	36.0	36.0	36.0	32.0	36.0
19	34.423092224985616	36.0	36.0	36.0	32.0	36.0
20	34.32076808147113	36.0	36.0	36.0	32.0	36.0
21	34.30694661334643	36.0	36.0	36.0	32.0	36.0
22	34.29722676965301	36.0	36.0	36.0	32.0	36.0
23	34.253275970610765	36.0	36.0	36.0	32.0	36.0
24	34.207654335534855	36.0	36.0	36.0	32.0	36.0
25	33.696057449221215	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	13.0
5	14.0
6	60.0
7	16.0
8	81.0
9	99.0
10	130.0
11	38.0
12	85.0
13	97.0
14	192.0
15	361.0
16	590.0
17	911.0
18	1038.0
19	1244.0
20	1635.0
21	1998.0
22	2751.0
23	3757.0
24	5387.0
25	7565.0
26	11171.0
27	15593.0
28	21932.0
29	31081.0
30	44060.0
31	64066.0
32	98446.0
33	153271.0
34	364859.0
35	678077.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.105162706238715	21.191611777841647	13.221330550150926	24.48189496576871
2	11.153450474199396	21.348836778224562	48.81009912971866	18.687613617857384
3	17.59196553936103	28.50489840744661	30.817169151452443	23.085966901739912
4	9.802835101879232	18.266964039235702	41.5934323824958	30.336768476389263
5	9.404301549376058	42.031832286314476	37.18025871263354	11.383607451675925
6	27.179707527382895	40.870161649117605	20.559614732738712	11.390516090760789
7	25.74573475672537	33.3561987142858	23.736649078421415	17.161417450567406
8	22.327115243260426	43.19705187039355	20.65829641549291	13.817536470853117
9	27.155590348423193	15.052970816378433	22.027395409460834	35.76404342573754
10	14.579720780880947	30.63939032257637	36.095807209252726	18.685081687289955
11	31.60286818306458	21.841812789192783	29.761770717562392	16.79354831018025
12	25.12777982390364	25.16533874570424	34.69285148394174	15.014029946450384
13	29.602181684738078	24.77884078457453	26.997752472578245	18.62122505810915
14	18.11187143529038	26.257261905550518	29.262250014904712	26.3686166442544
15	18.801483192865412	40.2754557810873	23.43513614481134	17.48792488123594
16	16.996432841703623	29.369802034306986	35.16290685311721	18.47085827087218
17	17.321047220853274	33.39977160601773	31.37138398274592	17.907797190383075
18	17.391540539716903	28.2219180659548	38.30699496110525	16.07954643322305
19	22.977933504385277	26.575787869091606	29.565619486936917	20.880659139586204
20	23.843886021690594	29.03757097143917	31.250024844144402	15.868518162725834
21	20.513044734077155	27.769364384715978	28.16260770136827	23.554983179838594
22	22.326830900303758	33.56063692706211	28.23573058793522	15.87680158469891
23	17.39171050983962	33.44497027542192	31.618563401501433	17.54475581323703
24	22.062831664933118	28.167056438523154	32.95217399945675	16.817937897086978
25	19.007443182382815	31.122969852958228	32.623796321140034	17.245790643518934
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	134.0
1	134.0
2	445.0
3	756.0
4	756.0
5	756.0
6	1817.5
7	2879.0
8	2879.0
9	2879.0
10	3471.0
11	4063.0
12	4063.0
13	4063.0
14	5923.0
15	7783.0
16	7783.0
17	7783.0
18	14513.0
19	21243.0
20	21243.0
21	21243.0
22	38077.0
23	54911.0
24	54911.0
25	54911.0
26	86873.5
27	118836.0
28	118836.0
29	118836.0
30	158390.5
31	197945.0
32	197945.0
33	197945.0
34	211867.0
35	225789.0
36	225789.0
37	225789.0
38	225752.5
39	225716.0
40	225716.0
41	225716.0
42	219325.0
43	212934.0
44	212934.0
45	212934.0
46	204516.5
47	196099.0
48	196099.0
49	196099.0
50	164781.0
51	133463.0
52	133463.0
53	133463.0
54	95798.5
55	58134.0
56	58134.0
57	58134.0
58	43048.5
59	27963.0
60	27963.0
61	27963.0
62	20185.5
63	12408.0
64	12408.0
65	12408.0
66	8943.0
67	5478.0
68	5478.0
69	5478.0
70	3772.0
71	2066.0
72	2066.0
73	2066.0
74	1438.0
75	810.0
76	810.0
77	810.0
78	575.5
79	341.0
80	341.0
81	341.0
82	241.0
83	141.0
84	141.0
85	141.0
86	107.5
87	74.0
88	74.0
89	74.0
90	58.0
91	42.0
92	42.0
93	42.0
94	44.5
95	47.0
96	47.0
97	47.0
98	306.5
99	566.0
100	566.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03965256672586969
2	0.004964845583372666
3	0.0018535423511257952
4	0.0038394805844748615
5	0.009400107637852248
6	0.012511410870099117
7	0.021977716449063
8	0.033827147908045765
9	0.04852309083482885
10	0.05554007259266223
11	0.05395132200598297
12	0.0657345555238541
13	0.0636824193493934
14	0.06825007728609625
15	0.05990913670603017
16	0.06725710816942171
17	0.06282184611494213
18	0.07698820551283214
19	0.07533325698504126
20	0.08016570668619064
21	0.07434028786836672
22	0.07851075815839977
23	0.08367419756510733
24	0.07884174786395794
25	0.07851075815839977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1510621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.26474825343052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.22831022367174	44.620985535925335
2	11.581624352090495	12.569478596639822
3	2.926266318049229	4.763793152143034
4	1.0633345110091406	2.3080631819638255
5	0.5038733331873865	1.367127978851522
6	0.32577205983045376	1.0606763288820646
7	0.20495085487086379	0.7785124580724958
8	0.15206663669862375	0.6601486206557358
9	0.11192427207725326	0.5466188202948549
>10	0.7410447012202668	7.681968066223149
>50	0.0833755787588804	3.1607114046448497
>100	0.06201481988733036	6.9951133951729085
>500	0.008824193513400958	3.338331268689382
>1k	0.005637679189117279	5.802907732054812
>5k	6.127912162083998E-4	2.115087842420281
>10k+	3.676747297250399E-4	2.230475617366015
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	11860	0.7851075815839976	No Hit
GTCCTACAGTGGACATTTCTAAATT	10966	0.7259266222301954	No Hit
CTGTAGGACGTGGAATATGGCAAGA	10712	0.7091123451878399	No Hit
CTTTAGGACGTGAAATATGGCGAGG	9613	0.6363608079061526	No Hit
GTCCTACAGTGTGCATTTCTCATTT	6675	0.44187125692016727	No Hit
CTGTAGGACCTGGAATATGGCGAGA	5364	0.35508575612281307	No Hit
CTGAAGGACCTGGAATATGGCGAGA	5106	0.3380066873160111	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5045	0.33396861290820135	No Hit
ATTTAGAAATGTCCACTGTAGGACG	4819	0.3190078782169717	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	4225	0.2796863011966602	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	3791	0.2509563947542104	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3565	0.2359956600629807	No Hit
GAATATGGCAAGAAAACTGAAAATC	3077	0.20369106480050259	No Hit
GGAATATGGCGAGAAAACTGAAAAT	2784	0.18429506805479337	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	2447	0.16198636190017218	No Hit
GCCATATTCCACGTCCTACAGTGGA	2429	0.16079479896016274	No Hit
CTGTAGGACATGGAATATGGCAAGA	2333	0.15443979661344573	No Hit
GTCCACTGTAGGACGTGGAATATGG	2132	0.14113401045000698	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	2123	0.14053822898000226	No Hit
GAAATATGGCGAGGAAAACTGAAAA	2094	0.13861848868776483	No Hit
ACAGTGGACATTTCTAAATTTTCCA	2083	0.13789031133553684	No Hit
GACCTGGAATATGGCGAGAAAACTG	1987	0.13153530898881982	No Hit
ACCTGGAATATGGCGAGAAAACTGA	1962	0.12988036046102894	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	1954	0.12935077693213584	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1925	0.12743103663989844	No Hit
CCATATTTCACGTCCTAAAGTGTGT	1908	0.12630567164100062	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1867	0.12359155605542356	No Hit
ATACACACTTTAGGACGTGAAATAT	1861	0.12319436840875375	No Hit
CCTAAAGTGTGTATTTCTCATTTTC	1840	0.12180421164540942	No Hit
CCACTGTAGGACGTGGAATATGGCA	1792	0.1186267104720509	No Hit
CACTTTAGGACGTGAAATATGGCGA	1792	0.1186267104720509	No Hit
GAAATACACACTTTAGGACGTGAAA	1713	0.1133970731242317	No Hit
GTGTATTTCTCATTTTCCGTGATTT	1664	0.11015337400976154	No Hit
ATTCCACGTCCTACAGTGGACATTT	1606	0.10631389342528669	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1599	0.10585050783750523	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.323958822232711E-4	0.0
5	0.0	0.0	0.0	1.9859382333490663E-4	0.0
6	0.0	0.0	0.0	1.9859382333490663E-4	0.0
7	0.0	0.0	0.0	1.9859382333490663E-4	0.0
8	0.0	0.0	0.0	1.9859382333490663E-4	0.0
9	0.0	0.0	0.0	1.9859382333490663E-4	0.0
10	0.0	0.0	0.0	1.9859382333490663E-4	0.0
11	0.0	0.0	0.0	1.9859382333490663E-4	0.0
12	0.0	0.0	0.0	1.9859382333490663E-4	0.0
13	0.0	0.0	0.0	2.647917644465422E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACGA	140	0.0	14.93042	19
TAGGACC	1895	0.0	14.385831	4
CTCGAAC	60	2.5651696E-5	14.251764	18
TGACGAG	40	0.0052746776	14.251763	18
GACGTTA	60	2.5683767E-5	14.249875	7
TCCAACG	220	0.0	13.819892	18
AAAGGCG	60	4.0967838E-4	12.664878	5
TAGAAAT	1545	0.0	12.480439	4
AATGTCC	1555	0.0	12.402231	8
GTATCAA	4065	0.0	12.36029	1
CCACCTT	1135	0.0	12.305488	13
AAATGTC	1510	0.0	12.268105	7
TCGAACT	70	1.0896008E-4	12.215797	19
ATTTAGA	1490	0.0	12.111582	1
GTCGGGA	55	0.0030655332	12.092405	12
GCGTGCA	55	0.0030670546	12.091604	9
GTTAGAC	55	0.0030754344	12.087201	3
ACCTTTT	1165	0.0	12.070963	15
ATAGGAC	425	0.0	12.066886	3
GAAATGT	1520	0.0	12.061596	6
>>END_MODULE