FastQCFastQC Report
Thu 2 Feb 2017
SRR4064181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510621
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA55950.3703774805196009No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA47460.3141754285158223No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA41540.2749862473777341No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC41220.2728679132621617No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA27070.17919782658919742No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA24530.16238354954684198No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT16940.1121393122431106No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA16400.10856462342308229No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA15950.1055857160730587No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA15640.10353357989859799No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCGT300.005742463729.33646832
TAGGACC23750.027.0468354
GTATCAA42700.025.964831
ATCTCGC609.759526E-625.6651642
GCGAGAT703.216673E-521.99943720
CAGGACG1105.2605174E-921.998714
AATGGCG1600.021.99870916
TGTAGGA74400.020.6970252
AGGACCT48400.020.58975
GTAGGAC73900.020.480533
GGTATCA18200.020.4266971
CTGTAGG73150.020.328961
GGACCTG46750.020.1399946
TGGGCGG550.00448478419.9994919
ATAACGG901.0013064E-519.55764635
GTCCTAC64000.019.2138611
GACCTGG48000.019.0197167
TTAGGAC43500.018.9644053
TCACGAC708.1278104E-418.85603742
GACGTTA708.1278104E-418.8560377
Produced by FastQC (version 0.11.5)