##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064181_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1510621
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255644532943737	32.0	32.0	32.0	32.0	32.0
2	31.329913989015115	32.0	32.0	32.0	32.0	32.0
3	31.446969822344585	32.0	32.0	32.0	32.0	32.0
4	31.533543489730384	32.0	32.0	32.0	32.0	32.0
5	31.48564001162436	32.0	32.0	32.0	32.0	32.0
6	35.07443693686239	36.0	36.0	36.0	36.0	36.0
7	35.105775704164046	36.0	36.0	36.0	36.0	36.0
8	35.02266418909839	36.0	36.0	36.0	36.0	36.0
9	35.1757144909279	36.0	36.0	36.0	36.0	36.0
10	35.024599154917084	36.0	36.0	36.0	36.0	36.0
11	35.182303833986154	36.0	36.0	36.0	36.0	36.0
12	35.12101380822854	36.0	36.0	36.0	36.0	36.0
13	35.144103650088276	36.0	36.0	36.0	36.0	36.0
14	35.094310882742924	36.0	36.0	36.0	36.0	36.0
15	35.04687807199821	36.0	36.0	36.0	36.0	36.0
16	35.092380550780106	36.0	36.0	36.0	36.0	36.0
17	35.096811840958125	36.0	36.0	36.0	36.0	36.0
18	35.08771822978762	36.0	36.0	36.0	36.0	36.0
19	35.05911012755681	36.0	36.0	36.0	36.0	36.0
20	35.048819657611006	36.0	36.0	36.0	36.0	36.0
21	35.024546196564195	36.0	36.0	36.0	36.0	36.0
22	34.99564549943368	36.0	36.0	36.0	36.0	36.0
23	34.97455020153963	36.0	36.0	36.0	36.0	36.0
24	34.977032624331315	36.0	36.0	36.0	36.0	36.0
25	34.92748478936808	36.0	36.0	36.0	36.0	36.0
26	34.88366704818747	36.0	36.0	36.0	36.0	36.0
27	34.828531445014995	36.0	36.0	36.0	32.0	36.0
28	34.77154362345022	36.0	36.0	36.0	32.0	36.0
29	34.76363760334326	36.0	36.0	36.0	32.0	36.0
30	34.69808443017805	36.0	36.0	36.0	32.0	36.0
31	34.68912851072506	36.0	36.0	36.0	32.0	36.0
32	34.65631683923367	36.0	36.0	36.0	32.0	36.0
33	34.6984624204218	36.0	36.0	36.0	32.0	36.0
34	34.672609476500064	36.0	36.0	36.0	32.0	36.0
35	34.64917739128477	36.0	36.0	36.0	32.0	36.0
36	34.5864429264521	36.0	36.0	36.0	32.0	36.0
37	34.484446462746114	36.0	36.0	36.0	32.0	36.0
38	34.435459324344095	36.0	36.0	36.0	32.0	36.0
39	34.26869678099272	36.0	36.0	36.0	32.0	36.0
40	34.36430977723731	36.0	36.0	36.0	32.0	36.0
41	34.34071616904571	36.0	36.0	36.0	32.0	36.0
42	34.32830670300492	36.0	36.0	36.0	32.0	36.0
43	34.312639636282036	36.0	36.0	36.0	32.0	36.0
44	34.20716447077063	36.0	36.0	36.0	32.0	36.0
45	34.16054192282512	36.0	36.0	36.0	32.0	36.0
46	34.003031865702916	36.0	36.0	36.0	32.0	36.0
47	34.01725316939193	36.0	36.0	36.0	32.0	36.0
48	33.96473039895513	36.0	36.0	36.0	32.0	36.0
49	33.90248778482491	36.0	36.0	36.0	32.0	36.0
50	33.45613161739443	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	2.0
20	4.0
21	32.0
22	85.0
23	290.0
24	825.0
25	2027.0
26	4552.0
27	9125.0
28	15950.0
29	25427.0
30	38956.0
31	57746.0
32	86681.0
33	141774.0
34	292460.0
35	834683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.27450622114355	21.877743307818502	13.484065477916495	24.363684993121456
2	10.893252677388135	21.967160373199242	48.21622201303576	18.923364936376863
3	17.28356568826045	29.22078351934967	31.01183619970608	22.4838145926838
4	9.82278149185004	18.971800339065855	41.67372226389015	29.531695905193956
5	9.450947656625983	42.434998586673956	36.598657108566606	11.51539664813345
6	27.504714948355673	41.08032391976545	20.188584694638827	11.226376437240049
7	25.980656948802476	33.40999059988614	23.7220479008619	16.887304550449482
8	22.518553627945064	43.30980437846422	20.457149741728735	13.714492251861982
9	27.172297648419402	14.948421191300682	21.995417717848724	35.88386344243119
10	14.580706064283108	30.975990319072107	35.70583589688594	18.73746771975885
11	31.667394844500933	21.90862030159802	29.70601474891104	16.717970104990005
12	25.17206568185805	25.17729533997743	34.551027230631234	15.09961174753329
13	29.63215741361654	24.803805592277072	26.956300695277896	18.607736298828492
14	18.343539966066892	26.33125890777224	29.130177761566618	26.19502336459425
15	19.035699674157556	40.14310057422945	23.410541618725944	17.41065813288705
16	17.168793736557557	29.574601468151545	34.7629793851081	18.493625410182794
17	17.559136275743555	33.5133034692355	30.99175769435219	17.93580256066876
18	17.61337570889814	28.38849504739448	37.87679090492043	16.12133833878695
19	23.25460853516534	26.590322787780657	29.625498387749143	20.529570289304864
20	24.05620783154862	29.123770031669366	30.93668244839164	15.883339688390368
21	20.77039839906899	27.672460531132558	28.218063961774657	23.339077108023787
22	22.580147608364516	33.350302094704226	28.29350087613091	15.776049420800348
23	17.58036979738662	33.42978437201901	31.362673779020543	17.627172051573833
24	22.28978870139301	28.240228622722856	32.60296661417135	16.86701606171278
25	19.356107171157287	31.111424453840314	32.16207145141014	17.370396923592256
26	16.120740047836847	32.607042066107006	33.14644788557288	18.125770000483268
27	17.93734649427684	32.39895930567417	31.064791828033865	18.598902372015118
28	16.95792691726655	32.68968767915518	29.36099279605869	20.991392607519586
29	19.893405958330217	30.058566481595616	28.683806536646916	21.364221023427255
30	17.40894520890734	30.08048596096583	31.639231179670773	20.871337650456052
31	21.779099473888742	28.24966551187441	30.607997129459648	19.3632378847772
32	18.465551197391722	32.04349337172931	30.90962050874667	18.5813349221323
33	16.63662236262758	32.91430190520387	33.49389327177506	16.955182460393488
34	18.61186229176114	31.862291761138895	32.92406584204133	16.601780105058637
35	17.525305017311346	32.85945040613808	32.05114625604904	17.564098320501532
36	16.727330745234703	31.044273964186786	34.02572916059805	18.202666129980457
37	17.477341379728088	31.066489338813813	29.229967700998234	22.226201580459865
38	18.761163418544946	34.0467549456675	29.021511882710122	18.17056975307743
39	21.486993277349868	29.36159648873104	30.126740656641722	19.024669577277376
40	22.44209216266492	28.879709252676104	31.732038475032937	16.946160109626042
41	22.064378517721174	27.65665320151519	32.71389664536339	17.56507163540024
42	19.158773019780842	32.49225640356864	31.900373559432886	16.44859701721763
43	16.797111609368205	33.154199095855354	32.436933957986206	17.611755336790242
44	16.548457566529855	32.41010194624652	31.33132530120482	19.7101151860188
45	17.08297944595635	32.97875086968581	30.532906395657893	19.40536328869994
46	18.513551175123645	28.97093684856485	31.497470884220714	21.018041092090794
47	21.89422675908089	29.23433890069443	30.23970766389292	18.631726676331763
48	17.278544362622437	31.02861387161094	30.35411188696382	21.338729878802802
49	20.75892369521512	29.271163428356566	29.929704034057696	20.040208842370614
50	17.628877512989863	34.13919360572382	29.085244879355965	19.14668400193036
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22.0
1	24.5
2	27.0
3	81.0
4	135.0
5	173.0
6	211.0
7	211.0
8	211.0
9	327.0
10	443.0
11	698.5
12	954.0
13	1762.5
14	2571.0
15	3634.5
16	4698.0
17	5259.5
18	5821.0
19	6229.0
20	6637.0
21	7965.5
22	9294.0
23	11715.5
24	14137.0
25	18008.0
26	21879.0
27	30299.0
28	38719.0
29	51966.0
30	65213.0
31	83600.5
32	101988.0
33	128673.0
34	155358.0
35	181474.5
36	207591.0
37	204284.0
38	200977.0
39	181361.0
40	161745.0
41	142075.0
42	122405.0
43	108613.5
44	94822.0
45	85645.5
46	76469.0
47	69004.0
48	61539.0
49	55066.5
50	48594.0
51	41871.0
52	35148.0
53	29996.0
54	24844.0
55	20979.5
56	17115.0
57	14320.0
58	11525.0
59	9537.0
60	7549.0
61	6115.0
62	4681.0
63	3778.0
64	2875.0
65	2281.5
66	1688.0
67	1380.0
68	1072.0
69	862.0
70	652.0
71	519.5
72	387.0
73	320.5
74	254.0
75	202.5
76	151.0
77	126.5
78	102.0
79	79.0
80	56.0
81	37.5
82	19.0
83	16.0
84	13.0
85	9.0
86	5.0
87	5.5
88	6.0
89	4.0
90	2.0
91	4.5
92	7.0
93	5.0
94	3.0
95	4.0
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008407138521177714
2	4.633855877814488E-4
3	6.619794111163555E-5
4	0.0
5	0.0
6	0.0
7	6.619794111163555E-5
8	0.0
9	0.0013239588222327108
10	0.001456354704455982
11	6.619794111163555E-5
12	3.971876466698133E-4
13	1.323958822232711E-4
14	1.323958822232711E-4
15	0.004964845583372666
16	9.267711755628976E-4
17	0.0
18	1.323958822232711E-4
19	0.0
20	8.60573234451262E-4
21	0.0
22	1.323958822232711E-4
23	6.619794111163554E-4
24	6.619794111163554E-4
25	0.0018535423511257952
26	0.004633855877814489
27	0.006024012641158835
28	0.003905678525586497
29	0.0019859382333490662
30	0.0031113032322468708
31	0.006884585875610096
32	0.003045105291135235
33	0.00489864764226103
34	0.0023831258800188796
35	0.003376094996693413
36	0.007745159110061359
37	0.0031775011733585065
38	0.007083179698945004
39	0.0037070847022515905
40	0.002052136174460702
41	0.0036408867611399547
42	0.0011915629400094398
43	0.001456354704455982
44	0.0013901567633443465
45	0.0011915629400094398
46	9.267711755628976E-4
47	0.002052136174460702
48	0.0016549485277908887
49	5.295835288930844E-4
50	0.0013239588222327108
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1510621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.11075035441554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.66266946671944	56.98327194031712
2	11.151016983002837	15.190082678543085
3	2.6459036428016756	5.406434474301108
4	0.9286557812976095	2.530057683405848
5	0.43878192777270014	1.4942883171277788
6	0.262052360329886	1.0709149736528527
7	0.15534967448446285	0.7406688027515678
8	0.11951429666662229	0.6512166739235095
9	0.08655738098001592	0.5305939350535711
>10	0.4791079045188786	6.055744955046224
>50	0.03976936806159443	1.8672871566479055
>100	0.026121137082620056	3.4354569301989697
>500	0.00264134912181867	1.317365679527962
>1k	0.00176089941454578	2.353814172019867
>5k	9.782774525254334E-5	0.37280162748262347
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	5595	0.3703774805196009	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	4746	0.3141754285158223	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	4154	0.2749862473777341	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	4122	0.2728679132621617	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	2707	0.17919782658919742	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	2453	0.16238354954684198	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	1694	0.1121393122431106	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	1640	0.10856462342308229	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	1595	0.1055857160730587	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	1564	0.10353357989859799	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.619794111163555E-5	0.0
5	0.0	0.0	0.0	6.619794111163555E-5	0.0
6	0.0	0.0	0.0	6.619794111163555E-5	0.0
7	0.0	0.0	0.0	1.323958822232711E-4	0.0
8	0.0	0.0	0.0	1.323958822232711E-4	0.0
9	0.0	0.0	0.0	1.323958822232711E-4	0.0
10	0.0	0.0	0.0	1.323958822232711E-4	0.0
11	0.0	0.0	0.0	1.323958822232711E-4	0.0
12	0.0	0.0	0.0	1.323958822232711E-4	0.0
13	0.0	0.0	0.0	1.323958822232711E-4	0.0
14	0.0	0.0	0.0	1.323958822232711E-4	0.0
15	0.0	0.0	0.0	1.9859382333490663E-4	0.0
16	0.0	0.0	0.0	1.9859382333490663E-4	0.0
17	0.0	0.0	0.0	2.647917644465422E-4	0.0
18	0.0	0.0	0.0	2.647917644465422E-4	0.0
19	0.0	0.0	0.0	3.309897055581777E-4	0.0
20	0.0	0.0	0.0	3.9718764666981326E-4	0.0
21	0.0	0.0	0.0	3.9718764666981326E-4	0.0
22	0.0	0.0	0.0	7.28177352227991E-4	0.0
23	0.0	0.0	0.0	0.0015225526455676175	0.0
24	0.0	0.0	0.0	0.002052136174460702	0.0
25	0.0	0.0	0.0	0.0025155217622421506	0.0
26	0.0	0.0	0.0	0.0033760949966934128	0.0
27	0.0	0.0	0.0	0.003905678525586497	0.0
28	0.0	0.0	0.0	0.00609021058227047	0.0
29	0.0	0.0	0.0	0.008340940580066078	0.0
30	0.0	0.0	0.0	0.016086099690127437	0.0
31	0.0	0.0	0.0	0.03534970055361338	0.0
32	0.0	0.0	0.0	0.05944575111824872	0.0
33	6.619794111163555E-5	0.0	0.0	0.08314461403621425	0.0
34	6.619794111163555E-5	0.0	0.0	0.1117421245964408	0.0
35	6.619794111163555E-5	0.0	0.0	0.14530448074004002	0.0
36	6.619794111163555E-5	0.0	0.0	0.18985569510817074	0.0
37	6.619794111163555E-5	0.0	0.0	0.25929733533427646	0.0
38	6.619794111163555E-5	0.0	0.0	0.34595044024940735	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGCGT	30	0.0057424637	29.336468	32
TAGGACC	2375	0.0	27.046835	4
GTATCAA	4270	0.0	25.96483	1
ATCTCGC	60	9.759526E-6	25.66516	42
GCGAGAT	70	3.216673E-5	21.999437	20
CAGGACG	110	5.2605174E-9	21.99871	4
AATGGCG	160	0.0	21.998709	16
TGTAGGA	7440	0.0	20.697025	2
AGGACCT	4840	0.0	20.5897	5
GTAGGAC	7390	0.0	20.48053	3
GGTATCA	1820	0.0	20.426697	1
CTGTAGG	7315	0.0	20.32896	1
GGACCTG	4675	0.0	20.139994	6
TGGGCGG	55	0.004484784	19.99949	19
ATAACGG	90	1.0013064E-5	19.557646	35
GTCCTAC	6400	0.0	19.213861	1
GACCTGG	4800	0.0	19.019716	7
TTAGGAC	4350	0.0	18.964405	3
TCACGAC	70	8.1278104E-4	18.856037	42
GACGTTA	70	8.1278104E-4	18.856037	7
>>END_MODULE