FastQCFastQC Report
Thu 2 Feb 2017
SRR4064180_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064180_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893548
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33040.3697618930376432No Hit
TATCAACGCAGAGTACTTTTTTTTT24040.26903982774288565No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22100.24732862700157127No Hit
GTACATGGGGTGGTATCAACGCAAA15270.17089177078343862No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14160.1584693827304185No Hit
GGTATCAACGCAGAGTACTTTTTTT12530.1402274975714791No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12460.13944410373029764No Hit
GTATCAACGCAGAGTACATGGGGTG9150.1024007663830035No Hit
GTCCTAAAGTGTGTATTTCTCATTT8940.10005058485945914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGCG456.747315E-414.7809613
CGCGCTA456.753052E-414.77930416
ATGACCG456.764538E-414.77599055
TCTAATC400.00529279514.243493
ACGCCCC551.9569659E-413.81960917
CTAGAAC903.653622E-813.7159533
GTGTAAT852.715242E-713.4033851
CACGCGC500.001496245313.30286312
ACCGGCG500.001496877313.3021178
GCGTTAT500.001505750513.2916911
TTAGGCA655.4593333E-513.1507834
TAAGGCT751.48045565E-512.6637164
TCTATAC604.1047888E-412.6608793
TCGGCGT550.00306174412.09351313
ATTACAC550.003077200412.0853843
GTACTAT550.00308107512.0833561
AATACTG951.0396307E-611.9985495
GTTATTC951.0438616E-611.9945183
ACAATAC802.8765797E-511.86957553
GCATTAG802.8815302E-511.8675811