##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064180_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	893548
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.218043126950093	32.0	32.0	32.0	32.0	32.0
2	30.82583811949666	32.0	32.0	32.0	32.0	32.0
3	30.87096831955306	32.0	32.0	32.0	32.0	32.0
4	30.88629262222063	32.0	32.0	32.0	32.0	32.0
5	30.799425436574197	32.0	32.0	32.0	32.0	32.0
6	34.44739734183278	36.0	36.0	36.0	32.0	36.0
7	34.37875637346846	36.0	36.0	36.0	32.0	36.0
8	34.326226459015075	36.0	36.0	36.0	32.0	36.0
9	34.46214640959411	36.0	36.0	36.0	32.0	36.0
10	34.1637651250968	36.0	36.0	36.0	32.0	36.0
11	34.46548030995537	36.0	36.0	36.0	32.0	36.0
12	34.291628429586325	36.0	36.0	36.0	32.0	36.0
13	34.36610232466527	36.0	36.0	36.0	32.0	36.0
14	34.26684184845134	36.0	36.0	36.0	32.0	36.0
15	34.19873134963091	36.0	36.0	36.0	32.0	36.0
16	34.20153589958234	36.0	36.0	36.0	32.0	36.0
17	34.135512585781626	36.0	36.0	36.0	32.0	36.0
18	34.11642239700609	36.0	36.0	36.0	32.0	36.0
19	34.130709262401126	36.0	36.0	36.0	32.0	36.0
20	34.10896448763805	36.0	36.0	36.0	32.0	36.0
21	34.08814187933944	36.0	36.0	36.0	32.0	36.0
22	34.07659577325449	36.0	36.0	36.0	32.0	36.0
23	34.02557892804863	36.0	36.0	36.0	32.0	36.0
24	34.02828835160506	36.0	36.0	36.0	32.0	36.0
25	33.61518463473703	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	11.0
5	12.0
6	34.0
7	10.0
8	50.0
9	56.0
10	84.0
11	19.0
12	50.0
13	52.0
14	85.0
15	136.0
16	296.0
17	350.0
18	485.0
19	626.0
20	948.0
21	1419.0
22	2124.0
23	3243.0
24	4828.0
25	7050.0
26	9901.0
27	13204.0
28	17871.0
29	24143.0
30	31775.0
31	43574.0
32	61288.0
33	86705.0
34	185839.0
35	397277.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.08557798559556	17.15254305601116	11.211636402719668	25.55024255567361
2	15.887810719761832	19.875432293590304	39.16227378034449	25.074483206303373
3	18.94877620225398	24.46207737848757	28.754826362852953	27.834320056405492
4	11.986198267284902	15.596002735276901	36.917060897963204	35.500738099474994
5	13.545771696100694	37.66719979585144	34.405523651125336	14.381504856922527
6	33.18547619847106	36.29047603057878	17.446806129187515	13.077241641762646
7	29.532571561617093	31.036339376463584	21.11946413299961	18.311624928919716
8	27.610292718206388	34.14467305707255	19.559814333407225	18.68521989131384
9	27.613167756664552	14.018929126064666	18.633981285850407	39.733921831420375
10	15.109215487133435	27.49966127354429	33.27301576281758	24.1181074765047
11	36.42455072856492	21.148370148825887	23.37599951626828	19.051079606340917
12	24.616597479705842	23.920061904319304	30.202879984232762	21.260460631742088
13	29.820665466175722	20.309462377151252	25.452087868770214	24.417784287902812
14	22.708647132274304	20.217592151900153	26.51231025079651	30.561450465029033
15	24.210663047519226	28.677712232987098	23.112489194067823	23.999135525425856
16	24.176006307056916	26.387955671423835	25.540780856648837	23.895257164870408
17	22.788506977650467	26.688197233128108	26.434332145568728	24.0889636436527
18	23.31020579588408	25.83609127817444	27.971888562228752	22.881814363712728
19	24.784436174180275	25.428187763120025	26.495171303104186	23.292204759595517
20	25.116786972274646	25.109059040280446	26.397943698095457	23.37621028934945
21	25.602031148154204	24.81795151496146	25.553313159232786	24.02670417765155
22	24.956320139775553	25.493582420536253	25.893533140693947	23.656564298994244
23	23.682362468064994	25.668073057662777	26.483682765680772	24.165881708591456
24	24.18593242852759	25.609556684011636	26.591231343701487	23.613279543759283
25	24.009838059536836	25.678858587974133	26.52568882656724	23.785614525921787
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	108.0
1	108.0
2	345.5
3	583.0
4	583.0
5	583.0
6	1691.0
7	2799.0
8	2799.0
9	2799.0
10	2718.5
11	2638.0
12	2638.0
13	2638.0
14	2694.0
15	2750.0
16	2750.0
17	2750.0
18	4942.0
19	7134.0
20	7134.0
21	7134.0
22	11453.0
23	15772.0
24	15772.0
25	15772.0
26	23406.5
27	31041.0
28	31041.0
29	31041.0
30	39805.5
31	48570.0
32	48570.0
33	48570.0
34	56343.5
35	64117.0
36	64117.0
37	64117.0
38	71920.5
39	79724.0
40	79724.0
41	79724.0
42	89209.5
43	98695.0
44	98695.0
45	98695.0
46	108754.5
47	118814.0
48	118814.0
49	118814.0
50	119590.5
51	120367.0
52	120367.0
53	120367.0
54	109429.5
55	98492.0
56	98492.0
57	98492.0
58	88229.5
59	77967.0
60	77967.0
61	77967.0
62	66802.0
63	55637.0
64	55637.0
65	55637.0
66	44926.5
67	34216.0
68	34216.0
69	34216.0
70	25665.0
71	17114.0
72	17114.0
73	17114.0
74	12807.0
75	8500.0
76	8500.0
77	8500.0
78	6590.5
79	4681.0
80	4681.0
81	4681.0
82	3232.0
83	1783.0
84	1783.0
85	1783.0
86	1364.0
87	945.0
88	945.0
89	945.0
90	684.0
91	423.0
92	423.0
93	423.0
94	307.5
95	192.0
96	192.0
97	192.0
98	339.0
99	486.0
100	486.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03928160546495543
2	0.006490977541217708
3	0.002014441305895151
4	0.004140796017673365
5	0.009064985876528178
6	0.013205781894201542
7	0.021487373929548272
8	0.03737907756494335
9	0.0477870243120683
10	0.05562096272388277
11	0.054949482288584386
12	0.062335767076866604
13	0.06490977541217707
14	0.06636464968865691
15	0.05819497105919324
16	0.06490977541217707
17	0.06200002685921741
18	0.07531772215930202
19	0.073527107665173
20	0.07643685621813265
21	0.0731913674475238
22	0.07699642324754798
23	0.08046573882992296
24	0.07677259643578184
25	0.07710833665343103
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	893548.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.391422895717206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6657194445108	44.182290227931226
2	14.712722290604194	17.18193578435829
3	4.72938567606063	8.28466677143411
4	1.9253225510055798	4.496892931457115
5	0.9493413885449618	2.771669724546812
6	0.5313733517324913	1.861658765791595
7	0.3270385746501144	1.3367373400925184
8	0.21310699112236592	0.9954896352527935
9	0.14144357274998723	0.7433182325093081
>10	0.6812625847176892	7.156383119226255
>50	0.06703798159917508	2.786517726596922
>100	0.05258577680236178	5.785134303753083
>500	0.0023114626734853792	0.9146439518932656
>1k	0.0013483532261998046	1.5026614851566686
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3304	0.3697618930376432	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2404	0.26903982774288565	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2210	0.24732862700157127	No Hit
GTACATGGGGTGGTATCAACGCAAA	1527	0.17089177078343862	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1416	0.1584693827304185	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1253	0.1402274975714791	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1246	0.13944410373029764	No Hit
GTATCAACGCAGAGTACATGGGGTG	915	0.1024007663830035	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	894	0.10005058485945914	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	1.1191340588306392E-4
9	0.0	0.0	0.0	0.0	1.1191340588306392E-4
10	0.0	0.0	0.0	0.0	2.2382681176612784E-4
11	0.0	0.0	0.0	0.0	2.2382681176612784E-4
12	0.0	0.0	0.0	0.0	4.4765362353225567E-4
13	0.0	0.0	0.0	0.0	4.4765362353225567E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGCG	45	6.747315E-4	14.78096	13
CGCGCTA	45	6.753052E-4	14.779304	16
ATGACCG	45	6.764538E-4	14.7759905	5
TCTAATC	40	0.005292795	14.24349	3
ACGCCCC	55	1.9569659E-4	13.819609	17
CTAGAAC	90	3.653622E-8	13.715953	3
GTGTAAT	85	2.715242E-7	13.403385	1
CACGCGC	50	0.0014962453	13.302863	12
ACCGGCG	50	0.0014968773	13.302117	8
GCGTTAT	50	0.0015057505	13.291691	1
TTAGGCA	65	5.4593333E-5	13.150783	4
TAAGGCT	75	1.48045565E-5	12.663716	4
TCTATAC	60	4.1047888E-4	12.660879	3
TCGGCGT	55	0.003061744	12.093513	13
ATTACAC	55	0.0030772004	12.085384	3
GTACTAT	55	0.003081075	12.083356	1
AATACTG	95	1.0396307E-6	11.998549	5
GTTATTC	95	1.0438616E-6	11.994518	3
ACAATAC	80	2.8765797E-5	11.8695755	3
GCATTAG	80	2.8815302E-5	11.867581	1
>>END_MODULE