FastQCFastQC Report
Thu 2 Feb 2017
SRR4064180_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064180_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893548
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11360.12713362908316062No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9360.10475094790654783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGAC4500.024.4403083
ATAATAC1306.91216E-1121.996283
CTTACAC1306.91216E-1121.996283
CTAGCGG1351.1277734E-1021.18634629
GTCCTAC5750.020.6596871
TAGGACG6750.020.529864
GACGTGA4650.020.3406457
AGGACGT6700.020.026465
TAATACT2100.019.9013944
TACCGTC1252.2902896E-819.3567267
ATACCGT1152.0235166E-719.12726
TAGACGT708.130298E-418.8539544
TTTAGGA5950.018.8539522
TAGCGGC1557.185008E-1018.45468930
GGACGTG6950.018.3566066
GCTAGAT600.00741546618.33125713
ATTTCGT2900.018.20483642
CTATACT1355.531365E-817.9228954
CGCAATA1601.0968506E-917.8789836
GTATAGC1254.8518814E-717.5989911
Produced by FastQC (version 0.11.5)