##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064180_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	893548
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26363552937279	32.0	32.0	32.0	32.0	32.0
2	31.409801152260428	32.0	32.0	32.0	32.0	32.0
3	31.497370034961747	32.0	32.0	32.0	32.0	32.0
4	31.570351005206213	32.0	32.0	32.0	32.0	32.0
5	31.518005747872525	32.0	32.0	32.0	32.0	32.0
6	35.10452824022884	36.0	36.0	36.0	36.0	36.0
7	35.13699655754363	36.0	36.0	36.0	36.0	36.0
8	35.08005389749627	36.0	36.0	36.0	36.0	36.0
9	35.19588986825554	36.0	36.0	36.0	36.0	36.0
10	35.05077063571291	36.0	36.0	36.0	36.0	36.0
11	35.19682770259684	36.0	36.0	36.0	36.0	36.0
12	35.11214506663324	36.0	36.0	36.0	36.0	36.0
13	35.1393680026143	36.0	36.0	36.0	36.0	36.0
14	35.091458992689816	36.0	36.0	36.0	36.0	36.0
15	35.06035825719491	36.0	36.0	36.0	36.0	36.0
16	35.07855313872338	36.0	36.0	36.0	36.0	36.0
17	35.04084727401326	36.0	36.0	36.0	36.0	36.0
18	35.043835361950336	36.0	36.0	36.0	36.0	36.0
19	35.03685644195947	36.0	36.0	36.0	36.0	36.0
20	35.028498748808126	36.0	36.0	36.0	36.0	36.0
21	35.027950373119296	36.0	36.0	36.0	36.0	36.0
22	35.00298920707114	36.0	36.0	36.0	36.0	36.0
23	34.96654124904314	36.0	36.0	36.0	36.0	36.0
24	34.953005322601584	36.0	36.0	36.0	36.0	36.0
25	34.91222855403403	36.0	36.0	36.0	32.0	36.0
26	34.86942727195405	36.0	36.0	36.0	32.0	36.0
27	34.847134121502144	36.0	36.0	36.0	32.0	36.0
28	34.822933966613995	36.0	36.0	36.0	32.0	36.0
29	34.800365509183614	36.0	36.0	36.0	32.0	36.0
30	34.782839869822325	36.0	36.0	36.0	32.0	36.0
31	34.76470542153304	36.0	36.0	36.0	32.0	36.0
32	34.71692399289126	36.0	36.0	36.0	32.0	36.0
33	34.690640010385565	36.0	36.0	36.0	32.0	36.0
34	34.66718967531683	36.0	36.0	36.0	32.0	36.0
35	34.62768536217416	36.0	36.0	36.0	32.0	36.0
36	34.59087032817487	36.0	36.0	36.0	32.0	36.0
37	34.571715229623926	36.0	36.0	36.0	32.0	36.0
38	34.528641997967654	36.0	36.0	36.0	32.0	36.0
39	34.49686642463527	36.0	36.0	36.0	32.0	36.0
40	34.48600410946026	36.0	36.0	36.0	32.0	36.0
41	34.455559186523836	36.0	36.0	36.0	32.0	36.0
42	34.39862995608518	36.0	36.0	36.0	32.0	36.0
43	34.37364081168555	36.0	36.0	36.0	32.0	36.0
44	34.307026595101775	36.0	36.0	36.0	32.0	36.0
45	34.25460523665209	36.0	36.0	36.0	32.0	36.0
46	34.211159333354225	36.0	36.0	36.0	32.0	36.0
47	34.15789750522636	36.0	36.0	36.0	32.0	36.0
48	34.11102145603817	36.0	36.0	36.0	32.0	36.0
49	34.05963305832479	36.0	36.0	36.0	32.0	36.0
50	33.48971963453558	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	1.0
20	9.0
21	33.0
22	91.0
23	295.0
24	758.0
25	1668.0
26	3429.0
27	6364.0
28	10453.0
29	16031.0
30	22826.0
31	32089.0
32	45883.0
33	72398.0
34	151043.0
35	530175.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.19882391653255	17.965268155536705	11.807618352632533	26.02828957529822
2	15.272856685609918	20.557195712363995	38.58402365407041	25.585923947955674
3	18.15713607835764	25.112585390310944	29.20482930890924	27.52544922242218
4	11.723488833280362	16.28664604475641	36.80205204421027	35.187813077752956
5	13.18194433874845	38.229507536248754	34.08065375335181	14.507894371650993
6	33.37750182418851	36.5910952741207	17.10450921494984	12.926893686740948
7	29.418229350857484	31.257190436327985	21.056507316898475	18.268072895916056
8	27.60209860018712	34.33257082999459	19.4122755576645	18.653055012153796
9	27.53018358603833	13.840466821557593	18.518978615203487	40.110370977200596
10	15.159383082327688	27.71843198785951	33.16520039975871	23.956984530054086
11	36.648995520101344	21.016689664897314	23.37470776579184	18.9596070492095
12	24.574169490614942	24.071118731170568	30.063298222367465	21.29141355584703
13	29.910681810805702	20.322266204239956	25.421382423084626	24.345669561869716
14	22.720898714443514	20.190746276340366	26.494863705495987	30.593491303720132
15	24.20143144134616	28.83408598720768	23.07575223417888	23.888730337267276
16	24.216124386010268	26.306795099497393	25.570175291340856	23.906905223151483
17	22.966981068728263	26.689109034993088	26.40372984999127	23.940180046287384
18	23.43306716505604	25.696971053500384	28.06920748255544	22.800754298888137
19	24.93900719379373	25.3581228988258	26.490015085927116	23.21285482145335
20	25.2629705855691	24.900592138469303	26.58434252037679	23.2520947555848
21	25.660960575145374	24.66179768742138	25.776231383204934	23.901010354228312
22	24.951205755034984	25.2834766571018	26.187513149825193	23.577804438038026
23	23.711081764666382	25.514694361752134	26.73422566421201	24.03999820936947
24	24.38928581021192	25.386326806514287	26.683856642761857	23.54053074051194
25	24.26583162475043	25.396294240359563	26.730779561468694	23.607094573421314
26	23.4787903071463	26.16983199456509	27.133588439847205	23.217789258441403
27	24.00650040347718	25.946324664263337	26.36155360538477	23.685621326874713
28	23.397137808040416	25.768233407311158	26.772026309692887	24.06260247495554
29	23.57628493886573	25.731456870261045	26.463389384740214	24.228868806133015
30	23.526791560017863	25.93907839764345	26.82133028318685	23.71279975915184
31	24.149092626625986	25.611836258671367	26.23357785144909	24.00549326325356
32	23.637667972732654	26.151649146132783	26.125796426057303	24.08488645507726
33	23.44567703926546	25.763507433621186	26.658295169153494	24.132520357959862
34	23.919558959804103	25.888392477426457	26.77432279379177	23.417725768977665
35	24.204304962245125	25.98835849392352	26.466463800198536	23.34087274363282
36	23.441711062362895	26.238416080941935	26.558065093790574	23.761807762904596
37	24.162491200463787	26.136049503476727	25.883003381036424	23.81845591502306
38	23.71833615386682	26.48313052354474	26.216187175974426	23.582346146614018
39	24.26063317220686	25.929909423716367	25.935057711183312	23.874399692893462
40	24.684927674099775	25.984947259449932	26.31028790464732	23.019837161802972
41	23.940589760004297	25.914029519337088	26.602209697420083	23.543171023238532
42	24.18466552288734	26.68441651026436	26.081750004756422	23.049167962091886
43	23.59476999833247	26.109495185985583	26.45038761685302	23.84534719882892
44	23.257021604247434	26.58495312961653	26.037124745950884	24.120900520185153
45	23.641853583635317	26.593056335852914	25.861131193664704	23.90395888684706
46	23.61902907318995	26.04007887745121	25.969908386660666	24.370983662698173
47	24.268683843559113	25.938361054966503	25.907248362106976	23.885706739367404
48	24.060828603948174	26.426845523414517	25.49133937754539	24.020986495091922
49	23.98482895078474	26.418620683480615	25.516035024576293	24.080515341158353
50	23.64076088963698	27.017133110920355	25.3785814290015	23.96352457044116
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	17.5
2	15.0
3	56.0
4	97.0
5	141.5
6	186.0
7	176.5
8	167.0
9	261.5
10	356.0
11	547.5
12	739.0
13	1339.5
14	1940.0
15	2750.5
16	3561.0
17	4064.5
18	4568.0
19	4641.5
20	4715.0
21	5082.5
22	5450.0
23	6073.0
24	6696.0
25	7122.0
26	7548.0
27	9021.5
28	10495.0
29	12434.0
30	14373.0
31	17058.5
32	19744.0
33	23589.5
34	27435.0
35	31593.0
36	35751.0
37	38700.5
38	41650.0
39	42954.0
40	44258.0
41	46537.0
42	48816.0
43	50258.0
44	51700.0
45	55081.0
46	58462.0
47	60832.0
48	63202.0
49	64447.5
50	65693.0
51	64273.0
52	62853.0
53	61364.0
54	59875.0
55	58441.0
56	57007.0
57	54601.5
58	52196.0
59	47333.0
60	42470.0
61	37022.5
62	31575.0
63	27054.5
64	22534.0
65	18983.0
66	15432.0
67	13009.0
68	10586.0
69	9382.0
70	8178.0
71	6438.5
72	4699.0
73	4006.5
74	3314.0
75	2577.5
76	1841.0
77	1601.0
78	1361.0
79	1097.5
80	834.0
81	662.0
82	490.0
83	399.5
84	309.0
85	243.5
86	178.0
87	120.0
88	62.0
89	47.5
90	33.0
91	30.5
92	28.0
93	24.0
94	20.0
95	15.5
96	11.0
97	11.0
98	11.0
99	12.5
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00828159203534673
2	2.2382681176612784E-4
3	4.4765362353225567E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0017906144941290227
10	0.0010072206529475754
11	1.1191340588306392E-4
12	0.0
13	1.1191340588306392E-4
14	5.595670294153195E-4
15	0.0048122764529717485
16	7.833938411814475E-4
17	0.0
18	3.3574021764919175E-4
19	0.0
20	7.833938411814475E-4
21	0.0
22	0.0
23	8.953072470645113E-4
24	4.4765362353225567E-4
25	0.002238268117661278
26	0.007274371382399155
27	0.007274371382399155
28	0.004140796017673365
29	0.0025740083353104704
30	0.004364622829439492
31	0.010072206529475753
32	0.002797835147076598
33	0.004476536235322556
34	0.002685921741193534
35	0.0023501815235443422
36	0.007610111600048346
37	0.003916969205907238
38	0.011191340588306392
39	0.005259930076504005
40	0.0025740083353104704
41	0.0035812289882580454
42	0.0021263547117782145
43	0.0012310474647137032
44	0.002238268117661278
45	0.001566787682362895
46	0.001119134058830639
47	0.0024620949294274063
48	0.0023501815235443422
49	4.4765362353225567E-4
50	0.001678701088245959
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	893548.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.601147125466795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.79976332930377	48.07753283378775
2	14.328714447427252	17.939879224843903
3	4.460685624370074	8.377321111549369
4	1.8233288762488389	4.565699169606624
5	0.8702976725037279	2.72408163196786
6	0.45086588221332735	1.6934832855773934
7	0.2962254267680094	1.2980836066385841
8	0.18890156923217435	0.9460363942187919
9	0.12792627121612232	0.7207498193051557
>10	0.5580319389973182	6.189931156844143
>50	0.057626882168464504	2.546486795061279
>100	0.03637167501196799	4.374482365813804
>500	0.001080346747741204	0.418184680923209
>1k	1.8005779129020067E-4	0.12804792386220093
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1136	0.12713362908316062	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	936	0.10475094790654783	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1191340588306392E-4
2	0.0	0.0	0.0	0.0	1.1191340588306392E-4
3	0.0	0.0	0.0	0.0	1.1191340588306392E-4
4	0.0	0.0	0.0	0.0	1.1191340588306392E-4
5	0.0	0.0	0.0	0.0	1.1191340588306392E-4
6	0.0	0.0	0.0	0.0	1.1191340588306392E-4
7	0.0	0.0	0.0	0.0	1.1191340588306392E-4
8	0.0	0.0	0.0	0.0	1.1191340588306392E-4
9	0.0	0.0	0.0	0.0	1.1191340588306392E-4
10	0.0	0.0	0.0	0.0	2.2382681176612784E-4
11	0.0	0.0	0.0	0.0	2.2382681176612784E-4
12	0.0	0.0	0.0	0.0	2.2382681176612784E-4
13	0.0	0.0	0.0	0.0	6.714804352983835E-4
14	0.0	0.0	0.0	0.0	6.714804352983835E-4
15	0.0	0.0	0.0	1.1191340588306392E-4	8.953072470645113E-4
16	0.0	0.0	0.0	1.1191340588306392E-4	8.953072470645113E-4
17	0.0	0.0	0.0	1.1191340588306392E-4	8.953072470645113E-4
18	0.0	0.0	0.0	2.2382681176612784E-4	0.0010072206529475752
19	0.0	0.0	0.0	2.2382681176612784E-4	0.0010072206529475752
20	0.0	0.0	0.0	2.2382681176612784E-4	0.0011191340588306393
21	0.0	0.0	0.0	3.3574021764919175E-4	0.0011191340588306393
22	0.0	0.0	0.0	4.4765362353225567E-4	0.0011191340588306393
23	0.0	0.0	0.0	0.0020144413058951504	0.0011191340588306393
24	0.0	0.0	0.0	0.0034693155823749817	0.0011191340588306393
25	0.0	0.0	0.0	0.003916969205907238	0.0011191340588306393
26	0.0	0.0	0.0	0.004252709423556429	0.0011191340588306393
27	0.0	0.0	0.0	0.0058194971059193235	0.0011191340588306393
28	0.0	0.0	0.0	0.008169678629463667	0.0011191340588306393
29	0.0	0.0	0.0	0.013541522111850734	0.0011191340588306393
30	0.0	0.0	0.0	0.021487373929548272	0.0011191340588306393
31	0.0	0.0	0.0	0.04196752720614897	0.0011191340588306393
32	0.0	0.0	0.0	0.06446212178864481	0.0011191340588306393
33	0.0	0.0	0.0	0.08863541745938662	0.0011191340588306393
34	0.0	0.0	0.0	0.11236105950659618	0.0012310474647137032
35	0.0	0.0	0.0	0.14324915953032183	0.0012310474647137032
36	0.0	0.0	0.0	0.1829784186188095	0.0012310474647137032
37	0.0	0.0	0.0	0.24128530308388582	0.0012310474647137032
38	0.0	0.0	0.0	0.31436475712552653	0.0014548742764798309
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGAC	450	0.0	24.440308	3
ATAATAC	130	6.91216E-11	21.99628	3
CTTACAC	130	6.91216E-11	21.99628	3
CTAGCGG	135	1.1277734E-10	21.186346	29
GTCCTAC	575	0.0	20.659687	1
TAGGACG	675	0.0	20.52986	4
GACGTGA	465	0.0	20.340645	7
AGGACGT	670	0.0	20.02646	5
TAATACT	210	0.0	19.901394	4
TACCGTC	125	2.2902896E-8	19.356726	7
ATACCGT	115	2.0235166E-7	19.1272	6
TAGACGT	70	8.130298E-4	18.853954	4
TTTAGGA	595	0.0	18.853952	2
TAGCGGC	155	7.185008E-10	18.454689	30
GGACGTG	695	0.0	18.356606	6
GCTAGAT	60	0.007415466	18.331257	13
ATTTCGT	290	0.0	18.204836	42
CTATACT	135	5.531365E-8	17.922895	4
CGCAATA	160	1.0968506E-9	17.87898	36
GTATAGC	125	4.8518814E-7	17.598991	1
>>END_MODULE