##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064179_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3176
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.296599496221663	32.0	32.0	32.0	14.0	32.0
2	27.692695214105793	32.0	21.0	32.0	14.0	32.0
3	27.740554156171285	32.0	21.0	32.0	14.0	32.0
4	27.799433249370278	32.0	21.0	32.0	14.0	32.0
5	27.705604534005037	32.0	21.0	32.0	14.0	32.0
6	31.02550377833753	36.0	21.0	36.0	14.0	36.0
7	30.916876574307306	36.0	27.0	36.0	14.0	36.0
8	30.909005037783377	36.0	27.0	36.0	14.0	36.0
9	31.270465994962215	36.0	32.0	36.0	14.0	36.0
10	30.008186397984886	36.0	21.0	36.0	14.0	36.0
11	31.56675062972292	36.0	32.0	36.0	14.0	36.0
12	30.711586901763223	36.0	27.0	36.0	14.0	36.0
13	31.26668765743073	36.0	32.0	36.0	14.0	36.0
14	30.689231738035264	36.0	32.0	36.0	14.0	36.0
15	30.294395465994963	36.0	21.0	36.0	14.0	36.0
16	30.402392947103273	36.0	21.0	36.0	14.0	36.0
17	30.29596977329975	36.0	21.0	36.0	14.0	36.0
18	30.465680100755666	36.0	21.0	36.0	14.0	36.0
19	30.4757556675063	36.0	27.0	36.0	14.0	36.0
20	30.236146095717885	36.0	21.0	36.0	14.0	36.0
21	30.196158690176322	36.0	21.0	36.0	14.0	36.0
22	29.989294710327457	36.0	21.0	36.0	14.0	36.0
23	29.95623425692695	36.0	21.0	36.0	14.0	36.0
24	29.941435768261965	36.0	21.0	36.0	14.0	36.0
25	29.327770780856422	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	4.0
15	9.0
16	18.0
17	16.0
18	55.0
19	69.0
20	95.0
21	75.0
22	81.0
23	105.0
24	119.0
25	102.0
26	103.0
27	113.0
28	126.0
29	145.0
30	191.0
31	200.0
32	247.0
33	292.0
34	461.0
35	548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.10236220472441	18.677165354330707	13.165354330708661	27.055118110236222
2	15.144836272040301	20.52896725440806	38.885390428211586	25.440806045340054
3	17.474811083123427	26.164987405541563	28.8727959697733	27.48740554156171
4	11.429471032745592	16.05793450881612	38.25566750629723	34.25692695214106
5	12.720403022670027	37.21662468513854	35.54785894206549	14.515113350125944
6	31.769521410579344	37.531486146095716	17.72670025188917	12.97229219143577
7	27.015113350125947	30.66750629722922	22.76448362720403	19.552896725440803
8	26.102015113350124	34.8551637279597	20.182619647355164	18.860201511335013
9	27.21259842519685	13.95275590551181	19.65354330708661	39.181102362204726
10	14.488188976377952	29.133858267716533	33.22834645669291	23.1496062992126
11	35.71653543307087	20.094488188976378	25.669291338582678	18.519685039370078
12	24.98424700693132	23.377441713925645	31.34845620667927	20.28985507246377
13	29.111531190926275	19.974795211090107	25.89792060491493	25.015752993068684
14	22.211720226843102	20.006301197227476	26.370510396975426	31.411468178954
15	23.09388783868935	27.882797731569	25.9294265910523	23.09388783868935
16	21.73913043478261	26.717076244486453	28.071833648393195	23.471959672337743
17	20.19533711405167	27.032136105860115	29.111531190926275	23.66099558916194
18	22.05419029615627	26.40201638311279	29.6471329552615	21.89666036546944
19	22.999369880277253	25.078764965343414	27.756773787019533	24.165091367359796
20	22.589792060491494	25.677378701953373	28.67044738500315	23.062381852551987
21	22.999369880277253	24.354127284183996	27.945809703843732	24.700693131695022
22	23.66099558916194	25.488342785129177	27.882797731569	22.967863894139885
23	22.589792060491494	24.763705103969755	28.48141146817895	24.165091367359796
24	22.810333963453054	27.284183994959044	27.567737870195337	22.337744171392565
25	23.282923755513547	26.118462507876494	28.638941398865786	21.95967233774417
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	5.5
7	10.0
8	10.0
9	10.0
10	12.5
11	15.0
12	15.0
13	15.0
14	15.0
15	15.0
16	15.0
17	15.0
18	29.0
19	43.0
20	43.0
21	43.0
22	54.5
23	66.0
24	66.0
25	66.0
26	103.5
27	141.0
28	141.0
29	141.0
30	191.0
31	241.0
32	241.0
33	241.0
34	259.0
35	277.0
36	277.0
37	277.0
38	311.0
39	345.0
40	345.0
41	345.0
42	336.5
43	328.0
44	328.0
45	328.0
46	350.5
47	373.0
48	373.0
49	373.0
50	373.0
51	373.0
52	373.0
53	373.0
54	333.5
55	294.0
56	294.0
57	294.0
58	259.0
59	224.0
60	224.0
61	224.0
62	203.5
63	183.0
64	183.0
65	183.0
66	151.5
67	120.0
68	120.0
69	120.0
70	89.5
71	59.0
72	59.0
73	59.0
74	45.0
75	31.0
76	31.0
77	31.0
78	27.0
79	23.0
80	23.0
81	23.0
82	15.5
83	8.0
84	8.0
85	8.0
86	5.5
87	3.0
88	3.0
89	3.0
90	2.5
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031486146095717885
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.031486146095717885
10	0.031486146095717885
11	0.031486146095717885
12	0.06297229219143577
13	0.06297229219143577
14	0.06297229219143577
15	0.06297229219143577
16	0.06297229219143577
17	0.06297229219143577
18	0.06297229219143577
19	0.06297229219143577
20	0.06297229219143577
21	0.06297229219143577
22	0.06297229219143577
23	0.06297229219143577
24	0.06297229219143577
25	0.06297229219143577
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3176.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.47481108312343
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.88014981273409	88.66498740554157
2	2.8941096356826694	5.35264483627204
3	0.7150153217568949	1.9836272040302267
4	0.10214504596527069	0.3778337531486146
5	0.20429009193054137	0.9445843828715366
6	0.0	0.0
7	0.034048348655090224	0.2204030226700252
8	0.0	0.0
9	0.034048348655090224	0.28337531486146095
>10	0.1361933946203609	2.172544080604534
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTT	27	0.850125944584383	No Hit
GTATCAACGCAGAGTACTTTTTTTT	22	0.6926952141057935	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	10	0.3148614609571789	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	10	0.3148614609571789	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	9	0.28337531486146095	No Hit
TTGTAGAACAGTGTATATCAATGAG	7	0.2204030226700252	No Hit
CTGAAGGACCTGGAATATGGCGAGA	5	0.15743073047858944	No Hit
GCATATGCTTGTCTCAAAGATTAAG	5	0.15743073047858944	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5	0.15743073047858944	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5	0.15743073047858944	No Hit
GTGTATATCAATGAGTTACAATGAA	5	0.15743073047858944	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5	0.15743073047858944	No Hit
GTTCTATTTTGTTGGTTTTCGGAAC	4	0.12594458438287154	No Hit
CTGTAGGACCTGGAATATGGCGAGA	4	0.12594458438287154	No Hit
GATATACACTGTTCTACAAATCCCG	4	0.12594458438287154	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE