##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064179_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3176
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.942380352644836	32.0	32.0	32.0	21.0	32.0
2	29.779282115869016	32.0	32.0	32.0	21.0	32.0
3	30.760390428211586	32.0	32.0	32.0	32.0	32.0
4	31.15994962216625	32.0	32.0	32.0	32.0	32.0
5	30.796599496221663	32.0	32.0	32.0	32.0	32.0
6	34.503778337531486	36.0	36.0	36.0	32.0	36.0
7	34.550377833753146	36.0	36.0	36.0	32.0	36.0
8	34.30887909319899	36.0	36.0	36.0	32.0	36.0
9	34.6926952141058	36.0	36.0	36.0	32.0	36.0
10	34.255037783375315	36.0	36.0	36.0	32.0	36.0
11	34.899874055415616	36.0	36.0	36.0	36.0	36.0
12	34.53463476070529	36.0	36.0	36.0	32.0	36.0
13	34.662153652392945	36.0	36.0	36.0	32.0	36.0
14	34.51857682619647	36.0	36.0	36.0	32.0	36.0
15	34.511649874055415	36.0	36.0	36.0	32.0	36.0
16	34.50913098236776	36.0	36.0	36.0	32.0	36.0
17	34.5941435768262	36.0	36.0	36.0	32.0	36.0
18	34.66309823677582	36.0	36.0	36.0	32.0	36.0
19	34.420025188916874	36.0	36.0	36.0	32.0	36.0
20	34.59382871536524	36.0	36.0	36.0	32.0	36.0
21	34.24590680100756	36.0	36.0	36.0	32.0	36.0
22	34.487405541561714	36.0	36.0	36.0	32.0	36.0
23	34.257241813602015	36.0	36.0	36.0	32.0	36.0
24	34.37311083123426	36.0	36.0	36.0	32.0	36.0
25	34.2852644836272	36.0	36.0	36.0	32.0	36.0
26	34.21662468513854	36.0	36.0	36.0	32.0	36.0
27	34.13287153652393	36.0	36.0	36.0	32.0	36.0
28	34.34981108312343	36.0	36.0	36.0	32.0	36.0
29	34.08154911838791	36.0	36.0	36.0	32.0	36.0
30	34.32619647355164	36.0	36.0	36.0	32.0	36.0
31	34.11933249370277	36.0	36.0	36.0	32.0	36.0
32	33.971662468513856	36.0	36.0	36.0	32.0	36.0
33	33.919080604534	36.0	36.0	36.0	32.0	36.0
34	34.04754408060453	36.0	36.0	36.0	32.0	36.0
35	33.98992443324937	36.0	36.0	36.0	32.0	36.0
36	33.698362720403026	36.0	36.0	36.0	27.0	36.0
37	33.71788413098237	36.0	36.0	36.0	32.0	36.0
38	33.62814861460957	36.0	36.0	36.0	21.0	36.0
39	33.727329974811084	36.0	36.0	36.0	32.0	36.0
40	33.83879093198993	36.0	36.0	36.0	32.0	36.0
41	33.587846347607055	36.0	36.0	36.0	21.0	36.0
42	33.6492443324937	36.0	36.0	36.0	27.0	36.0
43	33.758186397984886	36.0	36.0	36.0	32.0	36.0
44	33.35642317380353	36.0	36.0	36.0	21.0	36.0
45	33.584382871536526	36.0	36.0	36.0	21.0	36.0
46	33.54691435768262	36.0	36.0	36.0	21.0	36.0
47	33.25346347607053	36.0	36.0	36.0	21.0	36.0
48	33.20528967254408	36.0	36.0	36.0	21.0	36.0
49	32.9735516372796	36.0	36.0	36.0	14.0	36.0
50	32.54471032745592	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	1.0
24	6.0
25	8.0
26	14.0
27	37.0
28	44.0
29	98.0
30	142.0
31	200.0
32	313.0
33	458.0
34	768.0
35	1087.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.2544080604534	18.860201511335013	12.594458438287154	26.29093198992443
2	17.663727959697734	21.977329974811084	36.14609571788413	24.212846347607055
3	21.095717884130984	25.629722921914354	28.778337531486148	24.496221662468514
4	13.09823677581864	17.443324937027707	38.066750629722925	31.39168765743073
5	17.09697732997481	36.460957178841305	32.714105793450884	13.727959697732997
6	35.484886649874056	36.74433249370277	15.648614609571787	12.122166246851386
7	27.455919395465994	30.258186397984886	21.91435768261965	20.37153652392947
8	26.511335012594454	36.42947103274559	19.0176322418136	18.041561712846345
9	26.574307304785894	12.751889168765743	19.33249370277078	41.34130982367758
10	14.357682619647354	27.17254408060453	33.75314861460957	24.716624685138537
11	37.68891687657431	19.521410579345087	22.8904282115869	19.899244332493705
12	24.181360201511335	24.181360201511335	29.691435768261965	21.945843828715365
13	28.778337531486148	19.615869017632242	25.56675062972292	26.03904282115869
14	22.418136020151135	18.734256926952142	26.574307304785894	32.27329974811083
15	22.858942065491185	30.163727959697734	23.047858942065492	23.929471032745592
16	24.1183879093199	25.440806045340054	24.307304785894207	26.133501259445847
17	22.134760705289672	26.574307304785894	26.416876574307306	24.87405541561713
18	23.142317380352644	26.542821158690177	27.455919395465994	22.858942065491185
19	25.787153652392945	24.842569269521412	24.68513853904282	24.68513853904282
20	27.1095717884131	24.748110831234257	26.60579345088161	21.536523929471034
21	25.850125944584384	24.716624685138537	23.110831234256928	26.322418136020154
22	23.929471032745592	26.102015113350124	25.976070528967256	23.992443324937028
23	24.905541561712845	25.850125944584384	25.31486146095718	23.929471032745592
24	24.52770780856423	26.511335012594454	26.322418136020154	22.638539042821158
25	24.023929471032744	26.889168765743072	26.416876574307306	22.670025188916874
26	23.64609571788413	27.361460957178842	26.542821158690177	22.44962216624685
27	22.858942065491185	28.400503778337534	26.133501259445847	22.607052896725442
28	22.575566750629722	25.440806045340054	26.416876574307306	25.56675062972292
29	22.921914357682617	25.913098236775816	26.479848866498738	24.68513853904282
30	22.040302267002517	25.47229219143577	26.889168765743072	25.598236775818638
31	22.79596977329975	27.55037783375315	25.15743073047859	24.496221662468514
32	23.457178841309823	27.83375314861461	25.629722921914354	23.07934508816121
33	24.08690176322418	26.574307304785894	27.455919395465994	21.88287153652393
34	24.023929471032744	25.81863979848866	26.322418136020154	23.835012594458437
35	23.551637279596978	27.676322418136017	25.8816120906801	22.8904282115869
36	23.047858942065492	27.39294710327456	26.416876574307306	23.142317380352644
37	23.614609571788414	26.637279596977333	26.731738035264485	23.016372795969772
38	24.401763224181362	27.361460957178842	25.15743073047859	23.07934508816121
39	24.181360201511335	25.8816120906801	25.787153652392945	24.14987405541562
40	25.503778337531486	25.031486146095716	26.637279596977333	22.82745591939547
41	24.87405541561713	24.464735516372794	27.329974811083126	23.33123425692695
42	24.307304785894207	27.83375314861461	26.03904282115869	21.819899244332493
43	22.229219143576824	28.683879093198993	27.07808564231738	22.0088161209068
44	24.08690176322418	27.07808564231738	24.401763224181362	24.43324937027708
45	22.953400503778337	28.243073047858942	25.976070528967256	22.82745591939547
46	22.921914357682617	27.581863979848865	25.0	24.496221662468514
47	24.370277078085643	28.463476070528966	25.31486146095718	21.851385390428213
48	23.362720403022667	27.015113350125947	26.66876574307305	22.953400503778337
49	23.110831234256928	28.43198992443325	25.503778337531486	22.953400503778337
50	24.905541561712845	27.07808564231738	24.779596977329973	23.2367758186398
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.5
8	2.0
9	2.5
10	3.0
11	3.0
12	3.0
13	4.0
14	5.0
15	5.5
16	6.0
17	8.0
18	10.0
19	15.0
20	20.0
21	21.5
22	23.0
23	21.5
24	20.0
25	25.0
26	30.0
27	41.0
28	52.0
29	68.5
30	85.0
31	85.0
32	85.0
33	99.0
34	113.0
35	131.5
36	150.0
37	152.0
38	154.0
39	154.5
40	155.0
41	171.5
42	188.0
43	182.5
44	177.0
45	180.5
46	184.0
47	198.0
48	212.0
49	223.5
50	235.0
51	203.0
52	171.0
53	169.5
54	168.0
55	181.0
56	194.0
57	171.5
58	149.0
59	143.0
60	137.0
61	125.5
62	114.0
63	96.5
64	79.0
65	66.5
66	54.0
67	55.5
68	57.0
69	52.0
70	47.0
71	36.0
72	25.0
73	22.0
74	19.0
75	16.0
76	13.0
77	12.5
78	12.0
79	10.0
80	8.0
81	6.0
82	4.0
83	5.0
84	6.0
85	3.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3176.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.56801007556675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.45679817411151	94.11209068010076
2	2.0215194000652104	3.9042821158690177
3	0.22823606129768503	0.6612090680100755
4	0.19563090968373	0.7556675062972292
5	0.06521030322791001	0.3148614609571789
6	0.0	0.0
7	0.0	0.0
8	0.032605151613955004	0.2518891687657431
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	8	0.2518891687657431	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	5	0.15743073047858944	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	5	0.15743073047858944	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	4	0.12594458438287154	No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG	4	0.12594458438287154	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	4	0.12594458438287154	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.12594458438287154	No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTT	4	0.12594458438287154	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	4	0.12594458438287154	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.031486146095717885	0.0
29	0.0	0.0	0.0	0.031486146095717885	0.0
30	0.0	0.0	0.0	0.031486146095717885	0.0
31	0.0	0.0	0.0	0.031486146095717885	0.0
32	0.0	0.0	0.0	0.031486146095717885	0.0
33	0.0	0.0	0.0	0.031486146095717885	0.0
34	0.0	0.0	0.0	0.031486146095717885	0.0
35	0.0	0.0	0.0	0.09445843828715365	0.0
36	0.0	0.0	0.0	0.09445843828715365	0.0
37	0.0	0.0	0.0	0.09445843828715365	0.0
38	0.0	0.0	0.0	0.2518891687657431	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE