FastQCFastQC Report
Thu 2 Feb 2017
SRR4064178_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064178_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978038
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29160.2981479247227613No Hit
TATCAACGCAGAGTACTTTTTTTTT21460.21941887738513224No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17840.18240600058484435No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11040.11287904968927587No Hit
GGTATCAACGCAGAGTACTTTTTTT10910.11154985798097823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC453.547762E-516.882753
CGTCGTA1001.8189894E-1216.1507410
CCGTCGT1152.5465852E-1114.0441219
GTCGTAG1205.0931703E-1113.4589511
ACCGTCG1251.0186341E-1012.9205918
AGCGCAT604.08803E-412.66724710
ACCCGAG604.095096E-412.6646551
TAATACT1850.012.3217364
GAATAAA701.09171975E-412.2123451
GAACCGC951.0398016E-611.9987076
AGAACCG951.0404437E-611.9980945
AACCGCG658.015664E-411.6928437
CGACCCG658.0294424E-411.690455
GACAGTC907.448718E-611.6116447
GTCATAT907.4657128E-611.6092661
CCGACCA1001.9259132E-611.4005229
TACCGTC1501.7644197E-1011.4005227
TATACTG1001.9306626E-611.3981895
CCTAAGC752.0793235E-411.3958563
ATAATAC1104.983722E-711.2231913
Produced by FastQC (version 0.11.5)