FastQCFastQC Report
Thu 2 Feb 2017
SRR4064178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences978038
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10800.1104251573047264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA300.005748301329.3293466
TATACCG450.001399130924.441125
ACCGTCG2050.020.3885148
GTGTAAT1452.9649527E-1019.7224661
CGTCGTA2350.019.65891310
ATACCGT2350.019.6569026
GTATCAA21450.019.382971
CGTTATT2000.018.6993744
GGCGTTA1901.8189894E-1218.52569242
TTCGCCG750.0012904817.60030737
GCGTTAT2003.6379788E-1217.59940743
TACCGTC2400.017.415197
GTCGTAG2550.017.2543211
TGACGAA1551.4359102E-817.03429621
CGAACGT1053.7692836E-516.76391226
CGGCGTT2109.094947E-1216.7613441
ATCACGA2007.2759576E-1116.50113134
CCTAGTG800.001989792316.4977572
ATTATAC1206.4123033E-616.4977573
AACGCAG25350.015.96626956
Produced by FastQC (version 0.11.5)