##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064178_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	978038
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30292892505199	32.0	32.0	32.0	32.0	32.0
2	31.412625071827474	32.0	32.0	32.0	32.0	32.0
3	31.479910801011822	32.0	32.0	32.0	32.0	32.0
4	31.563260323218525	32.0	32.0	32.0	32.0	32.0
5	31.51097810105538	32.0	32.0	32.0	32.0	32.0
6	35.09094227422656	36.0	36.0	36.0	36.0	36.0
7	35.10778415562586	36.0	36.0	36.0	36.0	36.0
8	35.05907336933739	36.0	36.0	36.0	36.0	36.0
9	35.17283173046446	36.0	36.0	36.0	36.0	36.0
10	35.0237598130134	36.0	36.0	36.0	36.0	36.0
11	35.173607773931074	36.0	36.0	36.0	36.0	36.0
12	35.07596535103953	36.0	36.0	36.0	36.0	36.0
13	35.11268069338819	36.0	36.0	36.0	36.0	36.0
14	35.05380670280705	36.0	36.0	36.0	36.0	36.0
15	35.035385128185204	36.0	36.0	36.0	36.0	36.0
16	35.0509080424278	36.0	36.0	36.0	36.0	36.0
17	35.01119077172871	36.0	36.0	36.0	36.0	36.0
18	35.006455781881684	36.0	36.0	36.0	36.0	36.0
19	35.007466990035155	36.0	36.0	36.0	36.0	36.0
20	34.99486420773017	36.0	36.0	36.0	36.0	36.0
21	34.995021665824844	36.0	36.0	36.0	36.0	36.0
22	34.984799159132876	36.0	36.0	36.0	36.0	36.0
23	34.93312836515555	36.0	36.0	36.0	36.0	36.0
24	34.9062101881522	36.0	36.0	36.0	32.0	36.0
25	34.88672219279823	36.0	36.0	36.0	32.0	36.0
26	34.828039401332056	36.0	36.0	36.0	32.0	36.0
27	34.81956018068828	36.0	36.0	36.0	32.0	36.0
28	34.80551573660737	36.0	36.0	36.0	32.0	36.0
29	34.766626654588066	36.0	36.0	36.0	32.0	36.0
30	34.75204235418256	36.0	36.0	36.0	32.0	36.0
31	34.74029536684669	36.0	36.0	36.0	32.0	36.0
32	34.705818178843764	36.0	36.0	36.0	32.0	36.0
33	34.6677276343046	36.0	36.0	36.0	32.0	36.0
34	34.65292350603964	36.0	36.0	36.0	32.0	36.0
35	34.61421744349401	36.0	36.0	36.0	32.0	36.0
36	34.58370533660246	36.0	36.0	36.0	32.0	36.0
37	34.574284434756116	36.0	36.0	36.0	32.0	36.0
38	34.5461495361121	36.0	36.0	36.0	32.0	36.0
39	34.54158120645619	36.0	36.0	36.0	32.0	36.0
40	34.52134375146978	36.0	36.0	36.0	32.0	36.0
41	34.493533993566714	36.0	36.0	36.0	32.0	36.0
42	34.42584950686987	36.0	36.0	36.0	32.0	36.0
43	34.41803079225961	36.0	36.0	36.0	32.0	36.0
44	34.3615646835808	36.0	36.0	36.0	32.0	36.0
45	34.32284430666293	36.0	36.0	36.0	32.0	36.0
46	34.29725941118852	36.0	36.0	36.0	32.0	36.0
47	34.25396559233895	36.0	36.0	36.0	32.0	36.0
48	34.212692144885985	36.0	36.0	36.0	32.0	36.0
49	34.17832026976457	36.0	36.0	36.0	32.0	36.0
50	33.59586539582307	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	1.0
19	3.0
20	7.0
21	30.0
22	109.0
23	281.0
24	786.0
25	1826.0
26	3833.0
27	6956.0
28	11519.0
29	17297.0
30	25090.0
31	35054.0
32	50518.0
33	79853.0
34	164089.0
35	580784.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.710625905585	17.963329751042735	11.904895808515489	26.42114853485678
2	15.871550346460703	20.755127894457548	36.96204524796198	26.41127651111977
3	17.939133057610412	24.757191716042882	28.950395435746163	28.35327979060054
4	12.360460432007754	16.175547371370026	35.921917144323636	35.542075052298586
5	14.005284048268063	37.1838313030782	33.53816518376587	15.272719464887869
6	33.77530706915996	35.75110144094753	16.97481792611118	13.49877356378133
7	29.715031230924797	30.776749754866124	21.064336011827773	18.443883002381302
8	28.02130387571853	32.882260198478996	19.650565724440156	19.445870201362318
9	27.744269341706634	13.823618727052061	18.362338949049565	40.069772982191736
10	15.510685265978822	27.2226079185791	32.31703763385339	24.949669181588686
11	37.1176141597915	21.080797556738652	22.60906284731559	19.192525436154256
12	24.56553279681648	23.925577457703543	29.082948804595325	22.42594094088465
13	29.709324736486398	19.723363117604418	25.241888566132225	25.325423579776963
14	23.700583312458143	19.19491633734989	25.82013936106581	31.28436098912616
15	25.18982811685191	26.85109254695856	22.718841705947916	25.24023763024162
16	25.52897583080953	25.778561218459302	23.789955645169535	24.902507305561635
17	23.946308834626056	25.7060564108961	25.48469486870653	24.86293988577131
18	24.405952337132785	25.16860320070018	26.290136559390454	24.135307902776585
19	25.392264922221834	24.792594970747558	25.907480077461205	23.907660029569403
20	25.76902273659112	24.229494686752002	25.445207545394965	24.55627503126192
21	26.49181320153205	24.192618282725213	25.134401730812094	24.181166784930646
22	25.777628272112125	24.32011844120576	25.30586746118249	24.596385825499624
23	24.551037184877387	24.344090990887825	25.682084021790697	25.42278780244409
24	24.644210074281595	24.81373365983835	25.62750821800856	24.9145480478715
25	24.881852890233105	24.328079851291083	25.785108249541423	25.004959008934396
26	24.754162896158938	24.92860319943557	25.886695603715804	24.430538300689683
27	25.063600742758513	24.943761401097778	25.259413318936254	24.73322453720746
28	24.573338009548067	24.61495359402208	25.91802241106586	24.89368598536399
29	24.73489670403828	24.918533969315398	25.63866607362873	24.70790325301759
30	24.652535293498143	24.954474020283985	25.76325672562635	24.629733960591526
31	24.89414286137904	24.74618316122495	25.248653075915495	25.11102090148052
32	24.744533309339133	24.943098974043316	25.111194727273844	25.20117298934371
33	24.531950093763356	24.61293218567803	25.54166998979544	25.313447730763173
34	25.046855301218496	24.60739194139943	25.656766641683987	24.688986115698086
35	25.37964614075843	24.620660602689526	25.495901907535256	24.503791349016783
36	24.657451603535506	25.10920647385793	25.30328314743692	24.930058775169638
37	25.235607661735187	24.893072925134177	25.163828740811635	24.707490672319
38	24.692163435089995	24.821412868089908	25.573902248981035	24.912521447839058
39	25.057617940396366	24.929907545445065	25.116718507475504	24.89575600668307
40	25.14028457728535	24.90715860337092	25.458174337329194	24.494382482014533
41	24.597421905115393	25.206618752997162	25.561625711010837	24.634333630876608
42	25.262519657022033	25.15158145726783	25.25587358975565	24.33002529595449
43	24.823470228686062	24.589119672154613	25.421207293905457	25.166202805253867
44	24.508573946776195	25.105698139406	25.231360062820734	25.154367850997065
45	24.822678356892716	25.14588072902022	25.173998619667188	24.857442294419876
46	24.581224930498113	25.001150270287958	25.23815707273082	25.179467726483107
47	24.7203786429507	24.959126560935932	25.463308995019524	24.857185801093845
48	25.064109380623627	25.32688626770932	24.874030690704448	24.734973660962602
49	24.57235638024854	25.387154229812054	24.940237251465696	25.10025213847371
50	24.682573602638797	25.56547807718027	24.880115171233367	24.87183314894757
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	170.0
1	113.0
2	56.0
3	145.0
4	234.0
5	267.0
6	300.0
7	292.0
8	284.0
9	352.5
10	421.0
11	646.0
12	871.0
13	1571.5
14	2272.0
15	2888.0
16	3504.0
17	3601.0
18	3698.0
19	3688.5
20	3679.0
21	3921.0
22	4163.0
23	4720.5
24	5278.0
25	5761.0
26	6244.0
27	7571.5
28	8899.0
29	10173.0
30	11447.0
31	13368.0
32	15289.0
33	17754.0
34	20219.0
35	22864.5
36	25510.0
37	29508.0
38	33506.0
39	37412.0
40	41318.0
41	45103.5
42	48889.0
43	51823.0
44	54757.0
45	59711.0
46	64665.0
47	68449.0
48	72233.0
49	74521.5
50	76810.0
51	76272.5
52	75735.0
53	74511.0
54	73287.0
55	72427.5
56	71568.0
57	68686.0
58	65804.0
59	60090.5
60	54377.0
61	47812.0
62	41247.0
63	35482.0
64	29717.0
65	25133.0
66	20549.0
67	17383.0
68	14217.0
69	12165.0
70	10113.0
71	8093.0
72	6073.0
73	5140.0
74	4207.0
75	3296.5
76	2386.0
77	2017.0
78	1648.0
79	1366.5
80	1085.0
81	840.5
82	596.0
83	484.0
84	372.0
85	271.0
86	170.0
87	117.0
88	64.0
89	47.5
90	31.0
91	26.5
92	22.0
93	20.5
94	19.0
95	18.5
96	18.0
97	13.0
98	8.0
99	8.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00807739576580869
2	5.112275801144741E-4
3	3.0673654806868446E-4
4	0.0
5	0.0
6	1.022455160228948E-4
7	1.022455160228948E-4
8	0.0
9	9.202096442060534E-4
10	9.202096442060534E-4
11	1.022455160228948E-4
12	1.022455160228948E-4
13	5.112275801144741E-4
14	3.0673654806868446E-4
15	0.004907784769098951
16	0.0016359282563663169
17	0.0
18	2.044910320457896E-4
19	0.0
20	0.001124700676251843
21	0.0
22	0.0
23	6.134730961373689E-4
24	3.0673654806868446E-4
25	0.0020449103204578964
26	0.0054190123492134255
27	0.006339221993419479
28	0.003987575124892897
29	0.0023516468685265807
30	0.0025561379005723706
31	0.007054940605579742
32	0.0024538923845494756
33	0.004498802705007372
34	0.00276062893261816
35	0.002249401352503686
36	0.007566168185694216
37	0.0035785930608013185
38	0.008588623345923163
39	0.004498802705007372
40	0.002658383416595265
41	0.0029651199646639494
42	0.0016359282563663169
43	0.0016359282563663169
44	0.0017381737723892118
45	0.0013291917082976325
46	7.157186121602637E-4
47	0.0019426648044350014
48	0.002249401352503686
49	4.089820640915792E-4
50	0.0016359282563663169
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	978038.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.72326735944439
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.00851319535985	52.43135657934354
2	11.96645942665609	15.490167056348113
3	3.279218201899254	6.367251492344458
4	1.2584467162229658	3.2580313308685542
5	0.6781635072042128	2.194647899509838
6	0.41812835836706985	1.6237580117494468
7	0.26893406250138197	1.2184403860536934
8	0.19643690760365423	1.017123079207503
9	0.14678094578301695	0.8550128157467687
>10	0.684267576886533	7.756100346216238
>50	0.05473024191504273	2.469583699693885
>100	0.03833038743120267	4.669281219533168
>500	0.0014314249528465494	0.5380702834711198
>1k	1.5904721698294994E-4	0.11117579991368076
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1080	0.1104251573047264	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0224551602289482E-4
2	0.0	0.0	0.0	0.0	1.0224551602289482E-4
3	0.0	0.0	0.0	0.0	1.0224551602289482E-4
4	0.0	0.0	0.0	0.0	1.0224551602289482E-4
5	0.0	0.0	0.0	0.0	1.0224551602289482E-4
6	0.0	0.0	0.0	0.0	1.0224551602289482E-4
7	0.0	0.0	0.0	0.0	1.0224551602289482E-4
8	0.0	0.0	0.0	1.0224551602289482E-4	1.0224551602289482E-4
9	0.0	0.0	0.0	1.0224551602289482E-4	1.0224551602289482E-4
10	0.0	0.0	0.0	1.0224551602289482E-4	1.0224551602289482E-4
11	0.0	0.0	0.0	2.0449103204578964E-4	1.0224551602289482E-4
12	0.0	0.0	0.0	2.0449103204578964E-4	1.0224551602289482E-4
13	0.0	0.0	0.0	3.0673654806868446E-4	1.0224551602289482E-4
14	0.0	0.0	0.0	3.0673654806868446E-4	1.0224551602289482E-4
15	0.0	0.0	0.0	3.0673654806868446E-4	2.0449103204578964E-4
16	0.0	0.0	0.0	3.0673654806868446E-4	2.0449103204578964E-4
17	0.0	0.0	0.0	3.0673654806868446E-4	2.0449103204578964E-4
18	0.0	0.0	0.0	3.0673654806868446E-4	2.0449103204578964E-4
19	0.0	0.0	0.0	3.0673654806868446E-4	2.0449103204578964E-4
20	0.0	0.0	0.0	3.0673654806868446E-4	2.0449103204578964E-4
21	0.0	0.0	0.0	4.089820640915793E-4	2.0449103204578964E-4
22	0.0	0.0	0.0	9.202096442060534E-4	2.0449103204578964E-4
23	0.0	0.0	0.0	0.0014314372243205275	2.0449103204578964E-4
24	0.0	0.0	0.0	0.002862874448641055	2.0449103204578964E-4
25	0.0	0.0	0.0	0.003271856512732634	2.0449103204578964E-4
26	0.0	0.0	0.0	0.0037830840928471084	2.0449103204578964E-4
27	0.0	0.0	0.0	0.005214521317167636	2.0449103204578964E-4
28	0.0	0.0	0.0	0.00889535989399185	2.0449103204578964E-4
29	0.0	0.0	0.0	0.016666019111731854	2.0449103204578964E-4
30	0.0	0.0	0.0	0.02995793619470818	2.0449103204578964E-4
31	0.0	0.0	0.0	0.06349446545021768	2.0449103204578964E-4
32	0.0	0.0	0.0	0.09784895883391034	2.0449103204578964E-4
33	0.0	0.0	0.0	0.132203452217603	2.0449103204578964E-4
34	0.0	0.0	0.0	0.16942082004993672	2.0449103204578964E-4
35	0.0	0.0	0.0	0.20397980446567515	2.0449103204578964E-4
36	0.0	1.0224551602289482E-4	0.0	0.2612372934384963	2.0449103204578964E-4
37	0.0	1.0224551602289482E-4	0.0	0.34589658070545315	2.0449103204578964E-4
38	0.0	1.0224551602289482E-4	0.0	0.46225197793950745	2.0449103204578964E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGA	30	0.0057483013	29.329346	6
TATACCG	45	0.0013991309	24.44112	5
ACCGTCG	205	0.0	20.388514	8
GTGTAAT	145	2.9649527E-10	19.722466	1
CGTCGTA	235	0.0	19.658913	10
ATACCGT	235	0.0	19.656902	6
GTATCAA	2145	0.0	19.38297	1
CGTTATT	200	0.0	18.69937	44
GGCGTTA	190	1.8189894E-12	18.525692	42
TTCGCCG	75	0.00129048	17.600307	37
GCGTTAT	200	3.6379788E-12	17.599407	43
TACCGTC	240	0.0	17.41519	7
GTCGTAG	255	0.0	17.25432	11
TGACGAA	155	1.4359102E-8	17.034296	21
CGAACGT	105	3.7692836E-5	16.763912	26
CGGCGTT	210	9.094947E-12	16.76134	41
ATCACGA	200	7.2759576E-11	16.501131	34
CCTAGTG	80	0.0019897923	16.497757	2
ATTATAC	120	6.4123033E-6	16.497757	3
AACGCAG	2535	0.0	15.9662695	6
>>END_MODULE