FastQCFastQC Report
Thu 2 Feb 2017
SRR4064177_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064177_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923166
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19690.2132877510653555No Hit
TATCAACGCAGAGTACTTTTTTTTT15280.1655173609080057No Hit
GTATCTGATCGTCTTCGAACCTCCG11330.12272982323872414No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG11120.1204550427550408No Hit
CTATTGGAGCTGGAATTACCGCGGC10870.1177469707506559No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10870.1177469707506559No Hit
GATTAAGAGGGACGGCCGGGGGCAT10470.11341405554364005No Hit
GAATAGGACCGCGGTTCTATTTTGT10400.11265579538241226No Hit
GCGCAAGACGGACCAGAGCGAAAGC9820.10637306833223928No Hit
GAACTACGACGGTATCTGATCGTCT9740.1055064852908361No Hit
GTCCTATTCCATTATTCCTAGCTGC9660.10463990224943293No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAC453.5501558E-516.881233
CGCCAGT1550.014.09954918
TTTTCGG852.688539E-713.41440616
TATACTG500.00150019213.2982935
CTAGAAC500.001503872913.293973
ATTAGCT802.0070875E-613.0565773
CGGTTCT1650.012.66847412
CAAGACG2250.012.6622944
GTGTAAT751.483945E-512.6609231
CGCATCG1750.012.48749613
TCTAGAT1457.2759576E-1212.4419572
CGAACGA1001.4362558E-712.35243216
CTTATAC701.0945989E-412.2087483
CCTATAC701.0945989E-412.2087483
TAACACT550.003074760312.0867364
CTCGCGG951.0364529E-612.00171214
CTAGGAC951.0439435E-611.9945583
CGCAAGA2300.011.972182
GTCTTCG2550.011.9232711
GCCGGTC1603.6379788E-1211.8766948
Produced by FastQC (version 0.11.5)