##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064177_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	923166
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.03304714428391	32.0	32.0	32.0	21.0	32.0
2	28.25975068405899	32.0	32.0	32.0	14.0	32.0
3	28.441890190929907	32.0	32.0	32.0	14.0	32.0
4	28.38205804806503	32.0	27.0	32.0	14.0	32.0
5	28.27774853060013	32.0	21.0	32.0	14.0	32.0
6	31.59602823327549	36.0	32.0	36.0	21.0	36.0
7	31.46765153829322	36.0	32.0	36.0	21.0	36.0
8	31.129583411867422	36.0	32.0	36.0	14.0	36.0
9	31.915176685449854	36.0	32.0	36.0	21.0	36.0
10	30.38838410426727	36.0	21.0	36.0	14.0	36.0
11	32.058111975527694	36.0	32.0	36.0	21.0	36.0
12	31.146784002010474	36.0	32.0	36.0	14.0	36.0
13	31.626653277958678	36.0	32.0	36.0	21.0	36.0
14	31.0091402846292	36.0	32.0	36.0	14.0	36.0
15	30.923496965876126	36.0	32.0	36.0	14.0	36.0
16	31.068623627819914	36.0	32.0	36.0	14.0	36.0
17	30.69421317509527	36.0	27.0	36.0	14.0	36.0
18	30.90807720388316	36.0	32.0	36.0	14.0	36.0
19	30.902631812696743	36.0	32.0	36.0	14.0	36.0
20	30.781797639861086	36.0	32.0	36.0	14.0	36.0
21	30.73384418403624	36.0	32.0	36.0	14.0	36.0
22	30.7692061882695	36.0	32.0	36.0	14.0	36.0
23	30.516951447518647	36.0	27.0	36.0	14.0	36.0
24	30.497740384719542	36.0	27.0	36.0	14.0	36.0
25	29.502727570122815	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	6.0
5	14.0
6	35.0
7	34.0
8	64.0
9	63.0
10	60.0
11	41.0
12	49.0
13	31.0
14	403.0
15	1127.0
16	1720.0
17	2553.0
18	3912.0
19	5937.0
20	8536.0
21	11758.0
22	15474.0
23	20070.0
24	25119.0
25	31642.0
26	38817.0
27	47313.0
28	56786.0
29	66549.0
30	77527.0
31	91221.0
32	106868.0
33	113075.0
34	126627.0
35	69732.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.841117586249496	18.091750953030083	11.785064897068416	26.282066563652005
2	16.18410134305108	19.877437651794672	38.05954142572705	25.878919579427205
3	18.143392727347567	24.324898148418956	28.955854746267427	28.575854377966042
4	12.094872364280414	15.526439794829574	36.584464894083744	35.794222946806265
5	14.169444447453634	36.833427400528	34.007944976649306	14.989183175369055
6	33.62139569953979	36.08612187237288	16.97401263617691	13.31846979191042
7	29.809098690126657	30.54951841298389	21.411066208734656	18.2303166881548
8	27.606996997354944	33.46665178527891	19.530087673768197	19.396263543597954
9	27.09955968715355	13.906321626697501	18.945589989953977	40.04852869619497
10	15.52034889707038	26.47973997457404	32.06293846505665	25.936972663298935
11	36.645475373520924	20.789395538774	23.434116609190795	19.131012478514283
12	24.711504382575484	23.514059250122745	29.359408040700995	22.415028326600776
13	29.17589319875874	19.544206604950514	25.742485186064336	25.53741501022641
14	23.14699299259155	20.047691565638225	25.447677474948378	31.357637966821844
15	24.544843656857964	28.146317671923267	22.280604779710618	25.028233891508155
16	25.086278284319462	25.631587390363713	24.652393897220453	24.629740428096373
17	23.25965281932558	26.45355894874663	25.831859289510877	24.454928942416913
18	24.185339636488198	24.745122227509782	27.328208858310184	23.74132927769184
19	25.39297123215356	24.86116877302788	25.693234748618732	24.052625246199824
20	25.55583258355104	24.320914047848756	25.3256945874752	24.797558781124998
21	26.300442694670757	24.334982417954066	24.371512316103942	24.993062571271242
22	25.54170555542909	24.7576430645754	24.999485096525433	24.70116628347008
23	23.454191066949097	24.6009049695272	26.041289505692376	25.903614457831324
24	24.487141269884287	25.19964228611691	25.59226037234763	24.720956071651177
25	24.113186112761188	24.844772339991934	25.92095963647243	25.121081910774446
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	80.0
1	80.0
2	241.5
3	403.0
4	403.0
5	403.0
6	996.5
7	1590.0
8	1590.0
9	1590.0
10	1653.0
11	1716.0
12	1716.0
13	1716.0
14	2085.5
15	2455.0
16	2455.0
17	2455.0
18	4235.0
19	6015.0
20	6015.0
21	6015.0
22	10102.0
23	14189.0
24	14189.0
25	14189.0
26	21620.5
27	29052.0
28	29052.0
29	29052.0
30	36536.5
31	44021.0
32	44021.0
33	44021.0
34	53713.5
35	63406.0
36	63406.0
37	63406.0
38	71908.0
39	80410.0
40	80410.0
41	80410.0
42	90167.5
43	99925.0
44	99925.0
45	99925.0
46	111283.0
47	122641.0
48	122641.0
49	122641.0
50	123078.0
51	123515.0
52	123515.0
53	123515.0
54	112577.0
55	101639.0
56	101639.0
57	101639.0
58	92842.0
59	84045.0
60	84045.0
61	84045.0
62	73815.5
63	63586.0
64	63586.0
65	63586.0
66	52020.5
67	40455.0
68	40455.0
69	40455.0
70	30462.0
71	20469.0
72	20469.0
73	20469.0
74	15794.5
75	11120.0
76	11120.0
77	11120.0
78	9292.0
79	7464.0
80	7464.0
81	7464.0
82	5049.5
83	2635.0
84	2635.0
85	2635.0
86	2000.5
87	1366.0
88	1366.0
89	1366.0
90	931.0
91	496.0
92	496.0
93	496.0
94	296.0
95	96.0
96	96.0
97	96.0
98	236.5
99	377.0
100	377.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03466332165612685
2	0.004766206727717442
3	9.749059215785676E-4
4	0.0033580092854372887
5	0.007257632971751559
6	0.011048933777890435
7	0.01906482691086977
8	0.03390506149489907
9	0.04473734951243872
10	0.05275324264541805
11	0.049828524880682346
12	0.05795274089383708
13	0.06011919849734501
14	0.061852364580151344
15	0.05589460617050454
16	0.06174404169997595
17	0.05784441801366168
18	0.07203471531663862
19	0.06965161195277988
20	0.07322626699856796
21	0.06867670603120132
22	0.07181806955628782
23	0.07625930764347907
24	0.0694349661924291
25	0.07040987211400768
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	923166.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.07473680957897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2546867469673	43.39751063577462
2	11.70296221820083	12.656692036833128
3	3.35897820688919	5.449075874599334
4	1.494892334273831	3.233436381380582
5	0.7633761271763995	2.063968158188974
6	0.4872649939158451	1.5809235781512256
7	0.3403686586985847	1.2883741946148766
8	0.23710152770360865	1.0256962166177388
9	0.17595624645597654	0.8563308945397509
>10	0.9860478835977144	9.80072231147218
>50	0.09347975098118383	3.593814002062581
>100	0.09367618859385597	10.366338806394756
>500	0.009607814182009289	3.6044171485068146
>1k	0.0016013023636682147	1.0826997608634477
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1969	0.2132877510653555	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1528	0.1655173609080057	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1133	0.12272982323872414	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1112	0.1204550427550408	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1087	0.1177469707506559	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1087	0.1177469707506559	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1047	0.11341405554364005	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1040	0.11265579538241226	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	982	0.10637306833223928	No Hit
GAACTACGACGGTATCTGATCGTCT	974	0.1055064852908361	No Hit
GTCCTATTCCATTATTCCTAGCTGC	966	0.10463990224943293	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.083228801753964E-4
2	0.0	0.0	0.0	0.0	1.083228801753964E-4
3	0.0	0.0	0.0	0.0	1.083228801753964E-4
4	0.0	0.0	0.0	0.0	1.083228801753964E-4
5	0.0	0.0	0.0	0.0	1.083228801753964E-4
6	0.0	0.0	0.0	0.0	1.083228801753964E-4
7	0.0	0.0	0.0	0.0	1.083228801753964E-4
8	0.0	0.0	0.0	0.0	1.083228801753964E-4
9	0.0	0.0	0.0	0.0	1.083228801753964E-4
10	0.0	0.0	0.0	0.0	1.083228801753964E-4
11	0.0	0.0	0.0	0.0	1.083228801753964E-4
12	0.0	0.0	0.0	0.0	2.166457603507928E-4
13	0.0	0.0	0.0	0.0	2.166457603507928E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGTAC	45	3.5501558E-5	16.88123	3
CGCCAGT	155	0.0	14.099549	18
TTTTCGG	85	2.688539E-7	13.414406	16
TATACTG	50	0.001500192	13.298293	5
CTAGAAC	50	0.0015038729	13.29397	3
ATTAGCT	80	2.0070875E-6	13.056577	3
CGGTTCT	165	0.0	12.668474	12
CAAGACG	225	0.0	12.662294	4
GTGTAAT	75	1.483945E-5	12.660923	1
CGCATCG	175	0.0	12.487496	13
TCTAGAT	145	7.2759576E-12	12.441957	2
CGAACGA	100	1.4362558E-7	12.352432	16
CTTATAC	70	1.0945989E-4	12.208748	3
CCTATAC	70	1.0945989E-4	12.208748	3
TAACACT	55	0.0030747603	12.086736	4
CTCGCGG	95	1.0364529E-6	12.001712	14
CTAGGAC	95	1.0439435E-6	11.994558	3
CGCAAGA	230	0.0	11.97218	2
GTCTTCG	255	0.0	11.92327	11
GCCGGTC	160	3.6379788E-12	11.876694	8
>>END_MODULE