FastQCFastQC Report
Thu 2 Feb 2017
SRR4064177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923166
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG12340.13367043413643917No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10710.11601380466784955No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC10700.11590548178767417No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10390.11254747250223687No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9990.108214557295221No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9790.10604809969171308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC454.069539E-529.332253
CGCCCCT2050.020.3894925
GTATCAA15950.020.1371861
TATCGAG550.004483781819.99926210
TAGGACG5350.019.326394
CGGTCCA3100.019.16058310
GCATAAG708.1233523E-418.8564471
GTATTAC1051.7923849E-618.8564451
TAGCGGC3050.018.75645330
CTAGCGG3100.018.45193129
TCTAGCG3000.018.33364928
CGCCGGT3300.017.9993367
GTAGGAC6700.017.7306883
GGTCCAA3750.017.5993511
TCTAGAC1002.4827705E-517.599353
TAGGGTC1751.9099389E-1017.599354
GATTGCG750.001290876517.5993510
AATCGCT1509.591531E-917.5993515
AGGACGT5750.017.5993485
TAGGACA2750.017.5993484
Produced by FastQC (version 0.11.5)