FastQCFastQC Report
Thu 2 Feb 2017
SRR4064176_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064176_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1217809
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46530.3820796200389388No Hit
TATCAACGCAGAGTACTTTTTTTTT33210.27270286227150564No Hit
GTACTTTTTTTTTTTTTTTTTTTTT32480.26670849041187905No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21400.17572542163836857No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18470.15166581951685362No Hit
GGTATCAACGCAGAGTACTTTTTTT16020.1315477221797507No Hit
GTACATGGGGTGGTATCAACGCAAA14710.12079069870562625No Hit
GTACATGGAAGCAGTGGTATCAACG12310.10108317478356624No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC350.002168237516.287518
AAGACGG1800.013.1926465
TCCCCGA1352.7284841E-1112.66754313
CGAACGT751.4806554E-512.6644214
TACCGTC1301.9826984E-1012.4229157
CCGTCGT1405.2750693E-1112.2156329
ATCCCCG1251.4078978E-912.16084212
GCTCGTA550.003065253612.0922449
ATACCGT1501.4551915E-1112.0316946
TCGTTAA1157.0600436E-811.56601812
ACCGTCG1406.7848305E-1011.5369868
GTTATAT1406.7848305E-1011.5365121
GCGCCGA1251.8266292E-811.401257519
GTATAGG1001.9266517E-611.4007881
TAGGACG2600.011.3249154
AGCGTAT1104.95711E-711.2285128
CGCCAGT1701.0913936E-1111.17770418
AACCGCG855.3211283E-511.1763267
TGTAGGA3400.011.1726532
GTAGGAC3150.011.1558353
Produced by FastQC (version 0.11.5)