##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064176_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1217809
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.240678957044988	32.0	32.0	32.0	32.0	32.0
2	30.978887493851662	32.0	32.0	32.0	32.0	32.0
3	31.007153831183707	32.0	32.0	32.0	32.0	32.0
4	31.014764220004942	32.0	32.0	32.0	32.0	32.0
5	30.998785523838304	32.0	32.0	32.0	32.0	32.0
6	34.60002184250568	36.0	36.0	36.0	32.0	36.0
7	34.54162023765632	36.0	36.0	36.0	32.0	36.0
8	34.521088282316846	36.0	36.0	36.0	32.0	36.0
9	34.582791718569986	36.0	36.0	36.0	32.0	36.0
10	34.40659003177017	36.0	36.0	36.0	32.0	36.0
11	34.567816463829715	36.0	36.0	36.0	32.0	36.0
12	34.45994322590817	36.0	36.0	36.0	32.0	36.0
13	34.4949372192191	36.0	36.0	36.0	32.0	36.0
14	34.43350804600721	36.0	36.0	36.0	32.0	36.0
15	34.397744638116485	36.0	36.0	36.0	32.0	36.0
16	34.386999110697985	36.0	36.0	36.0	32.0	36.0
17	34.33915170605571	36.0	36.0	36.0	32.0	36.0
18	34.32753822643781	36.0	36.0	36.0	32.0	36.0
19	34.34099764412974	36.0	36.0	36.0	32.0	36.0
20	34.293772668784676	36.0	36.0	36.0	32.0	36.0
21	34.26302071999796	36.0	36.0	36.0	32.0	36.0
22	34.25902419837593	36.0	36.0	36.0	32.0	36.0
23	34.22039416690138	36.0	36.0	36.0	32.0	36.0
24	34.21448601545891	36.0	36.0	36.0	32.0	36.0
25	33.801850700725645	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	6.0
5	17.0
6	53.0
7	19.0
8	69.0
9	80.0
10	104.0
11	34.0
12	70.0
13	65.0
14	143.0
15	259.0
16	403.0
17	525.0
18	687.0
19	885.0
20	1230.0
21	1718.0
22	2506.0
23	3714.0
24	5560.0
25	7836.0
26	11414.0
27	15395.0
28	21082.0
29	29130.0
30	38905.0
31	53744.0
32	76033.0
33	110153.0
34	247323.0
35	588643.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.8705859545707	16.923697431499534	11.272142103758613	25.93357451017116
2	16.278286776196445	20.026343644965188	37.942814253488606	25.752555325349764
3	19.043497611659145	24.30408830415071	28.479763768988274	28.17265031520187
4	12.438863359402971	15.403395074907783	35.92921124290092	36.22853032278832
5	14.18575889022293	36.95000188882848	33.71016830283013	15.154070918118464
6	33.66786215550991	35.377819096558646	17.29281318755323	13.66150556037821
7	29.744012208433706	30.637714893977012	20.928051225483955	18.690221672105324
8	27.935887810239485	33.070908831033556	19.749360504564773	19.24384285416218
9	27.43555786318944	14.207503023292498	18.69692807192807	39.66001104158999
10	15.46204734346193	27.24376986200528	32.08582686739822	25.20835592713458
11	36.62214488352776	21.383354817825474	22.735604363282633	19.25889593536413
12	24.638099135118967	23.68037033932982	29.591979267833757	22.089551257717456
13	29.716589676212823	19.85712806708769	25.436183376963996	24.990098879735488
14	23.317712814166764	19.74660411885399	25.975584002813502	30.960099064165743
15	24.698650159764096	27.465457022266737	23.146526969280433	24.689365848688734
16	24.843941170525444	25.9885506187711	24.71863396244399	24.44887424825946
17	23.265272585349823	25.966311983895483	26.070580502033607	24.69783492872109
18	23.894014026056844	25.383974400063114	27.259758168033276	23.462253405846763
19	24.98307201788121	25.104444006179534	26.219307760575884	23.693176215363376
20	25.296518694257646	24.619125609863328	26.16793398807269	23.916421707806332
21	26.107967968708273	24.358572972262284	25.207098160542674	24.326360898486772
22	25.08511570229597	24.72361536495592	25.727417733814455	24.46385119893366
23	24.00086454360398	24.74304071162129	26.045197879402743	25.21089686537199
24	24.124284035533204	25.43122221400455	26.193164542399067	24.251329208063176
25	24.442505045723788	24.919630002169495	26.047521842888983	24.590343109217734
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	222.0
1	222.0
2	529.5
3	837.0
4	837.0
5	837.0
6	2456.5
7	4076.0
8	4076.0
9	4076.0
10	3916.5
11	3757.0
12	3757.0
13	3757.0
14	3643.0
15	3529.0
16	3529.0
17	3529.0
18	6092.5
19	8656.0
20	8656.0
21	8656.0
22	13787.5
23	18919.0
24	18919.0
25	18919.0
26	28323.0
27	37727.0
28	37727.0
29	37727.0
30	47671.5
31	57616.0
32	57616.0
33	57616.0
34	67910.0
35	78204.0
36	78204.0
37	78204.0
38	89274.0
39	100344.0
40	100344.0
41	100344.0
42	115067.0
43	129790.0
44	129790.0
45	129790.0
46	147488.0
47	165186.0
48	165186.0
49	165186.0
50	165997.5
51	166809.0
52	166809.0
53	166809.0
54	153214.0
55	139619.0
56	139619.0
57	139619.0
58	127090.0
59	114561.0
60	114561.0
61	114561.0
62	99120.0
63	83679.0
64	83679.0
65	83679.0
66	67668.0
67	51657.0
68	51657.0
69	51657.0
70	38814.0
71	25971.0
72	25971.0
73	25971.0
74	19457.5
75	12944.0
76	12944.0
77	12944.0
78	10374.0
79	7804.0
80	7804.0
81	7804.0
82	5353.0
83	2902.0
84	2902.0
85	2902.0
86	2253.5
87	1605.0
88	1605.0
89	1605.0
90	1105.5
91	606.0
92	606.0
93	606.0
94	406.0
95	206.0
96	206.0
97	206.0
98	394.5
99	583.0
100	583.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036541033938819634
2	0.00476265161449784
3	0.001478064294154502
4	0.0036951607353862548
5	0.010100106010055765
6	0.014452184209510686
7	0.0238132580724892
8	0.036376804572802464
9	0.048694007024089984
10	0.05427780546867366
11	0.05353877332159641
12	0.06265350313554917
13	0.06363887933165217
14	0.06733404006703843
15	0.057644507472025576
16	0.06569174640686676
17	0.062325044403514836
18	0.07644876988099118
19	0.07299995319463068
20	0.07702357266205127
21	0.07176823294950194
22	0.07685934329603411
23	0.08063661871442894
24	0.07546339368488819
25	0.07694145797904269
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1217809.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.396810774136206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.81765249207363	45.23881885749175
2	12.815596138302416	14.711486930157891
3	3.80442421821617	6.5508545087248375
4	1.5509448773078527	3.5607715857581885
5	0.8142254622422447	2.336697239190085
6	0.45812482844568814	1.5776942453538465
7	0.329474865333409	1.323756455026298
8	0.2275398811309966	1.0448050800676207
9	0.1623529123473324	0.8386685449766784
>10	0.8806653479096674	9.081335504401217
>50	0.06831085419655245	2.722838704391446
>100	0.06451010221987441	7.369636986851559
>500	0.004884946263338852	1.9372631943525058
>1k	0.0012930740108838138	1.7053721632559946
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4653	0.3820796200389388	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3321	0.27270286227150564	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3248	0.26670849041187905	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2140	0.17572542163836857	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1847	0.15166581951685362	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1602	0.1315477221797507	No Hit
GTACATGGGGTGGTATCAACGCAAA	1471	0.12079069870562625	No Hit
GTACATGGAAGCAGTGGTATCAACG	1231	0.10108317478356624	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.463440490257503E-4
2	0.0	0.0	0.0	0.0	2.463440490257503E-4
3	0.0	0.0	0.0	0.0	2.463440490257503E-4
4	0.0	0.0	0.0	0.0	2.463440490257503E-4
5	0.0	0.0	0.0	0.0	2.463440490257503E-4
6	0.0	0.0	0.0	0.0	3.284587320343338E-4
7	0.0	0.0	0.0	0.0	3.284587320343338E-4
8	0.0	0.0	0.0	0.0	3.284587320343338E-4
9	0.0	0.0	0.0	0.0	3.284587320343338E-4
10	0.0	0.0	0.0	8.211468300858345E-5	3.284587320343338E-4
11	0.0	0.0	0.0	8.211468300858345E-5	4.1057341504291726E-4
12	0.0	0.0	0.0	8.211468300858345E-5	5.748027810600841E-4
13	0.0	0.0	0.0	8.211468300858345E-5	5.748027810600841E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTCC	35	0.0021682375	16.28751	8
AAGACGG	180	0.0	13.192646	5
TCCCCGA	135	2.7284841E-11	12.667543	13
CGAACGT	75	1.4806554E-5	12.664421	4
TACCGTC	130	1.9826984E-10	12.422915	7
CCGTCGT	140	5.2750693E-11	12.215632	9
ATCCCCG	125	1.4078978E-9	12.160842	12
GCTCGTA	55	0.0030652536	12.092244	9
ATACCGT	150	1.4551915E-11	12.031694	6
TCGTTAA	115	7.0600436E-8	11.566018	12
ACCGTCG	140	6.7848305E-10	11.536986	8
GTTATAT	140	6.7848305E-10	11.536512	1
GCGCCGA	125	1.8266292E-8	11.4012575	19
GTATAGG	100	1.9266517E-6	11.400788	1
TAGGACG	260	0.0	11.324915	4
AGCGTAT	110	4.95711E-7	11.228512	8
CGCCAGT	170	1.0913936E-11	11.177704	18
AACCGCG	85	5.3211283E-5	11.176326	7
TGTAGGA	340	0.0	11.172653	2
GTAGGAC	315	0.0	11.155835	3
>>END_MODULE