##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1217809
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25418600125307	32.0	32.0	32.0	32.0	32.0
2	31.362122467480532	32.0	32.0	32.0	32.0	32.0
3	31.44817619183304	32.0	32.0	32.0	32.0	32.0
4	31.529013991520838	32.0	32.0	32.0	32.0	32.0
5	31.470839023196575	32.0	32.0	32.0	32.0	32.0
6	35.04653849659511	36.0	36.0	36.0	36.0	36.0
7	35.073260256739765	36.0	36.0	36.0	36.0	36.0
8	35.01867862694396	36.0	36.0	36.0	36.0	36.0
9	35.1403266029402	36.0	36.0	36.0	36.0	36.0
10	34.98555684840562	36.0	36.0	36.0	36.0	36.0
11	35.13909159810775	36.0	36.0	36.0	36.0	36.0
12	35.039433934221215	36.0	36.0	36.0	36.0	36.0
13	35.08109235520513	36.0	36.0	36.0	36.0	36.0
14	35.02450712714391	36.0	36.0	36.0	36.0	36.0
15	34.99799229600044	36.0	36.0	36.0	36.0	36.0
16	35.004412843064884	36.0	36.0	36.0	36.0	36.0
17	34.96770429517272	36.0	36.0	36.0	36.0	36.0
18	34.97172791464015	36.0	36.0	36.0	36.0	36.0
19	34.967531854338404	36.0	36.0	36.0	36.0	36.0
20	34.95235295518427	36.0	36.0	36.0	36.0	36.0
21	34.94278905805426	36.0	36.0	36.0	36.0	36.0
22	34.92700333139269	36.0	36.0	36.0	36.0	36.0
23	34.885362154492206	36.0	36.0	36.0	32.0	36.0
24	34.85780775146185	36.0	36.0	36.0	32.0	36.0
25	34.829507747109766	36.0	36.0	36.0	32.0	36.0
26	34.774271663290385	36.0	36.0	36.0	32.0	36.0
27	34.768461228320696	36.0	36.0	36.0	32.0	36.0
28	34.73934500401951	36.0	36.0	36.0	32.0	36.0
29	34.707972268229256	36.0	36.0	36.0	32.0	36.0
30	34.695257630712206	36.0	36.0	36.0	32.0	36.0
31	34.67407532708331	36.0	36.0	36.0	32.0	36.0
32	34.62927355603383	36.0	36.0	36.0	32.0	36.0
33	34.59491513036938	36.0	36.0	36.0	32.0	36.0
34	34.576636401931665	36.0	36.0	36.0	32.0	36.0
35	34.53467497776745	36.0	36.0	36.0	32.0	36.0
36	34.49353223699283	36.0	36.0	36.0	32.0	36.0
37	34.47121182385744	36.0	36.0	36.0	32.0	36.0
38	34.42908124344622	36.0	36.0	36.0	32.0	36.0
39	34.41691348971801	36.0	36.0	36.0	32.0	36.0
40	34.383735052048394	36.0	36.0	36.0	32.0	36.0
41	34.35313830001256	36.0	36.0	36.0	32.0	36.0
42	34.290171118787924	36.0	36.0	36.0	32.0	36.0
43	34.263130753673195	36.0	36.0	36.0	32.0	36.0
44	34.20569317520235	36.0	36.0	36.0	32.0	36.0
45	34.142609391127834	36.0	36.0	36.0	32.0	36.0
46	34.1143841111373	36.0	36.0	36.0	32.0	36.0
47	34.062714268000974	36.0	36.0	36.0	32.0	36.0
48	34.00353914283767	36.0	36.0	36.0	32.0	36.0
49	33.97326838609339	36.0	36.0	36.0	32.0	36.0
50	33.36429768543343	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	4.0
20	10.0
21	32.0
22	122.0
23	411.0
24	1098.0
25	2456.0
26	4893.0
27	9364.0
28	15476.0
29	23560.0
30	33658.0
31	47352.0
32	67097.0
33	106325.0
34	216327.0
35	689622.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00079821829792	17.910638968820216	11.857894149027027	26.230668663854832
2	15.544935355003469	20.835519643949567	37.35786928120676	26.261675719840206
3	18.378871511554383	24.863566118084492	28.738649669980276	28.01891270038085
4	12.192306018431461	16.07403131361322	35.94972610647483	35.783936561480495
5	13.8496266655937	37.38821112341919	33.42125078727452	15.340911423712585
6	33.84958876932981	35.80441251822947	16.896013164636788	13.449985547803923
7	29.566942517937996	30.842268801434713	20.91104822935673	18.67974045127056
8	28.051548355734234	33.21771576471825	19.541175620459054	19.189560259088463
9	27.56123798027574	14.067450052964798	18.521009369431514	39.850302597327946
10	15.390262408482208	27.541969262503734	32.06727563565654	25.000492693357508
11	37.047547725092954	21.232985823709484	22.562095174280348	19.157371276917214
12	24.631797459041163	23.904014425919357	29.428776950061092	22.035411164978388
13	29.838496443604317	19.784678347782815	25.42252214227882	24.954303066334045
14	23.454638916214897	19.590230299407292	25.931552316214567	31.023578468163237
15	24.77723424749889	27.51587136699462	23.113768816325724	24.593125569180767
16	24.9581621911023	26.078134468934916	24.56565046091388	24.398052879048908
17	23.433067090159458	26.02370322439726	26.010154301700844	24.533075383742442
18	24.13249729431453	25.16156103681539	27.254094658755175	23.45184701011491
19	25.177018727895756	25.088663328978516	26.218889825908658	23.51542811721707
20	25.395897365994337	24.550111963921914	26.202349641116008	23.85164102896774
21	26.2489437998898	24.370077737970405	25.21241015627245	24.168568305867343
22	25.136249011542883	24.77502592775374	25.822154085171135	24.266570975532247
23	24.15215684728805	24.599358184786286	26.21309316174466	25.035391806181
24	24.348890011126574	25.23302170708775	26.230472037805725	24.18761624397995
25	24.604897740653943	24.750161358399588	26.214786506003517	24.430154394942953
26	23.984085338063387	25.429297595145123	26.69787188615022	23.888745180641266
27	24.536740112126754	25.258575594405652	26.00430801927194	24.200376274195655
28	23.935415421202347	25.121289063783102	26.412356538934034	24.530938976080517
29	24.04052945480345	25.20182200247828	26.222777330242465	24.534871212475807
30	24.081326302944504	25.427813832106626	26.396969520291748	24.09389034465712
31	24.394436104550515	25.134843492857073	25.774980537205966	24.69573986538645
32	24.072302473449486	25.58958032408339	25.699370900816493	24.638746301650635
33	23.884017839619162	25.176166649832187	26.09598608568831	24.843829424860335
34	24.429818438621414	25.227341780467576	26.253972425006367	24.088867355904643
35	24.873169393791585	25.363821949372866	25.986191107322753	23.776817549512796
36	24.14589400068654	25.822489172263257	25.752850031124098	24.27876679592611
37	24.692690177335976	25.629832189826278	25.6318851647923	24.045592468045445
38	24.226908257151962	25.5786280454756	25.864657208460486	24.329806488911956
39	24.6576253649163	25.37321031907337	25.569803201819756	24.399361114190572
40	24.949333867640682	25.576871280104058	25.736013848195398	23.737781004059862
41	23.996291548656178	25.627073994115417	26.2676769377192	24.108957519509197
42	24.976124646180807	26.090362568700666	25.430721236870607	23.502791548247924
43	24.24658479144153	25.314258310970384	25.76778552588792	24.671371371700165
44	24.006871446953628	25.81982323761099	25.58045579174786	24.592849523687523
45	24.157488823998925	25.85638879673638	25.612421866531776	24.37370051273292
46	24.10561711946592	25.587065146432693	25.490661400317787	24.8166563337836
47	24.498594158531617	25.549115765132502	25.675822154484894	24.27646792185098
48	24.771182362688887	26.094161352360274	24.829156614238016	24.30549967071282
49	24.195680735752997	26.005091147971754	25.280587945475446	24.518640170799802
50	24.169191732564727	26.401678450307525	24.913901411573423	24.515228405554325
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	59.0
1	54.0
2	49.0
3	126.0
4	203.0
5	261.5
6	320.0
7	350.0
8	380.0
9	501.5
10	623.0
11	923.0
12	1223.0
13	2224.0
14	3225.0
15	4305.0
16	5385.0
17	5756.5
18	6128.0
19	6096.0
20	6064.0
21	6320.5
22	6577.0
23	7345.0
24	8113.0
25	8488.5
26	8864.0
27	10828.0
28	12792.0
29	14865.0
30	16938.0
31	19418.5
32	21899.0
33	25619.5
34	29340.0
35	33262.0
36	37184.0
37	42059.0
38	46934.0
39	50359.0
40	53784.0
41	58194.5
42	62605.0
43	64658.5
44	66712.0
45	73607.0
46	80502.0
47	85726.0
48	90950.0
49	92625.0
50	94300.0
51	92248.5
52	90197.0
53	88376.5
54	86556.0
55	85703.5
56	84851.0
57	81138.0
58	77425.0
59	70405.5
60	63386.0
61	55593.5
62	47801.0
63	41164.0
64	34527.0
65	28980.5
66	23434.0
67	19897.5
68	16361.0
69	14374.0
70	12387.0
71	9605.0
72	6823.0
73	5903.0
74	4983.0
75	3889.0
76	2795.0
77	2381.5
78	1968.0
79	1654.5
80	1341.0
81	1071.0
82	801.0
83	649.5
84	498.0
85	374.5
86	251.0
87	177.5
88	104.0
89	83.5
90	63.0
91	50.0
92	37.0
93	30.0
94	23.0
95	22.0
96	21.0
97	17.5
98	14.0
99	11.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007965124251832595
2	3.284587320343338E-4
3	2.463440490257503E-4
4	0.0
5	0.0
6	8.211468300858345E-5
7	2.463440490257503E-4
8	8.211468300858345E-5
9	0.0015601789771630854
10	0.001067490879111585
11	8.211468300858345E-5
12	8.211468300858345E-5
13	2.463440490257503E-4
14	2.463440490257503E-4
15	0.004105734150429172
16	9.032615130944179E-4
17	0.0
18	2.463440490257503E-4
19	0.0
20	4.926880980515006E-4
21	0.0
22	1.642293660171669E-4
23	0.001067490879111585
24	3.284587320343338E-4
25	0.0018886377091974192
26	0.005255339712549341
27	0.006569174640686675
28	0.004680536931489257
29	0.0022992111242403366
30	0.003941504784412005
31	0.00829358298386693
32	0.002956128588309004
33	0.005748027810600842
34	0.003202472637334754
35	0.003530931369369088
36	0.008457812349884096
37	0.005255339712549341
38	0.00829358298386693
39	0.005255339712549341
40	0.0027097845392832537
41	0.004105734150429172
42	0.00213498175822317
43	0.0017244083431802522
44	0.0013138349281373353
45	0.0017244083431802522
46	0.0011496055621201683
47	0.0027097845392832537
48	0.0022170964412317534
49	7.39032147077251E-4
50	0.0015601789771630854
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1217809.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.730451831317446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.11593349583225	49.45592773585505
2	12.24715719646457	15.12045094773858
3	3.530399437732666	6.537994573087996
4	1.43621029930274	3.5463164280299915
5	0.7395206246090784	2.282547114784825
6	0.42302587651580176	1.5668147096195726
7	0.28183378075983406	1.2178408639343115
8	0.2018532610067745	0.996839440445843
9	0.15429215875643967	0.8572072206657945
>10	0.7590127581385661	8.325439136064885
>50	0.056957599127856175	2.4907028321770057
>100	0.04992217306235144	5.8284896454469015
>500	0.003613660160689302	1.5606013059747075
>1k	2.676785304214298E-4	0.2128280461745379
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1429	0.11734188201926576	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.642293660171669E-4
7	0.0	0.0	0.0	0.0	1.642293660171669E-4
8	0.0	0.0	0.0	0.0	1.642293660171669E-4
9	0.0	0.0	0.0	0.0	2.463440490257503E-4
10	0.0	0.0	0.0	0.0	2.463440490257503E-4
11	0.0	0.0	0.0	0.0	2.463440490257503E-4
12	0.0	0.0	0.0	0.0	2.463440490257503E-4
13	0.0	0.0	0.0	0.0	2.463440490257503E-4
14	0.0	0.0	0.0	0.0	2.463440490257503E-4
15	0.0	0.0	0.0	0.0	2.463440490257503E-4
16	0.0	0.0	0.0	0.0	2.463440490257503E-4
17	0.0	0.0	0.0	0.0	2.463440490257503E-4
18	0.0	0.0	0.0	0.0	2.463440490257503E-4
19	0.0	0.0	0.0	8.211468300858345E-5	2.463440490257503E-4
20	0.0	0.0	0.0	8.211468300858345E-5	2.463440490257503E-4
21	0.0	0.0	0.0	1.642293660171669E-4	2.463440490257503E-4
22	0.0	0.0	0.0	4.926880980515006E-4	2.463440490257503E-4
23	0.0	0.0	0.0	9.853761961030013E-4	2.463440490257503E-4
24	0.0	0.0	0.0	0.001642293660171669	2.463440490257503E-4
25	0.0	0.0	0.0	0.002052867075214586	2.463440490257503E-4
26	0.0	0.0	0.0	0.0024634404902575033	3.284587320343338E-4
27	0.0	0.0	0.0	0.003284587320343338	3.284587320343338E-4
28	0.0	0.0	0.0	0.005994371859626591	3.284587320343338E-4
29	0.0	0.0	0.0	0.012235087768278934	3.284587320343338E-4
30	0.0	0.0	0.0	0.023484799340454866	3.284587320343338E-4
31	0.0	0.0	0.0	0.04450615819065223	3.284587320343338E-4
32	0.0	0.0	0.0	0.06774461348208134	6.569174640686676E-4
33	0.0	0.0	0.0	0.09237901838465638	6.569174640686676E-4
34	0.0	0.0	0.0	0.12087281338863483	6.569174640686676E-4
35	0.0	0.0	0.0	0.15388291595808537	6.569174640686676E-4
36	0.0	0.0	0.0	0.19756792731865178	7.39032147077251E-4
37	0.0	0.0	0.0	0.26301332967649277	9.032615130944179E-4
38	0.0	0.0	0.0	0.3507118111296599	9.032615130944179E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCA	30	0.0057480703	29.330156	4
TCATGCG	50	0.0025820713	21.997618	4
TTCGTAC	55	0.0044811117	20.001942	34
CGGGAAT	55	0.004484308	19.999477	40
GAACCGT	55	0.0044864393	19.997835	6
TTAACGG	80	8.979112E-5	19.252659	35
GGCGTTA	220	0.0	18.998724	42
TAGGACG	360	0.0	18.942394	4
CTATACT	140	4.087269E-9	18.855103	4
CCGAACG	60	0.0074114953	18.333607	9
GTAGGAC	590	0.0	18.269209	3
GTAGAAC	590	0.0	18.269209	3
TAGGACA	340	0.0	18.115686	4
GTAACAC	195	1.8189894E-12	18.049328	3
CGCAATA	305	0.0	18.035278	36
TGTAGGA	600	0.0	17.96546	2
TATACTG	200	3.6379788E-12	17.598095	5
ATACGAA	295	0.0	17.152094	40
CTAGCGG	270	0.0	17.110664	29
AGGACGT	390	0.0	16.921244	5
>>END_MODULE