##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064175_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4627
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.96239464015561	32.0	32.0	32.0	14.0	32.0
2	27.410200994164686	32.0	21.0	32.0	14.0	32.0
3	27.747784741733305	32.0	21.0	32.0	14.0	32.0
4	27.245299330019453	32.0	21.0	32.0	14.0	32.0
5	27.45731575534904	32.0	21.0	32.0	14.0	32.0
6	30.799438080829912	36.0	21.0	36.0	14.0	36.0
7	30.74454290036741	36.0	21.0	36.0	14.0	36.0
8	30.488437432461637	36.0	21.0	36.0	14.0	36.0
9	30.79706073049492	36.0	32.0	36.0	14.0	36.0
10	29.46963475253944	36.0	21.0	36.0	14.0	36.0
11	31.415604063107846	36.0	32.0	36.0	14.0	36.0
12	30.467689647719904	36.0	21.0	36.0	14.0	36.0
13	30.71104387291982	36.0	27.0	36.0	14.0	36.0
14	30.21007132051005	36.0	21.0	36.0	14.0	36.0
15	30.268208342338447	36.0	21.0	36.0	14.0	36.0
16	30.15496001728982	36.0	21.0	36.0	14.0	36.0
17	29.861465312297383	36.0	21.0	36.0	14.0	36.0
18	30.242705856926733	36.0	21.0	36.0	14.0	36.0
19	30.00778041927815	36.0	21.0	36.0	14.0	36.0
20	29.96585260427923	36.0	21.0	36.0	14.0	36.0
21	29.743462286578776	36.0	21.0	36.0	14.0	36.0
22	29.69418629781716	36.0	21.0	36.0	14.0	36.0
23	29.7084503998271	36.0	21.0	36.0	14.0	36.0
24	29.61443700021612	36.0	21.0	36.0	14.0	36.0
25	29.04300842878755	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	4.0
15	8.0
16	28.0
17	42.0
18	81.0
19	89.0
20	118.0
21	124.0
22	164.0
23	152.0
24	165.0
25	173.0
26	184.0
27	195.0
28	233.0
29	228.0
30	256.0
31	275.0
32	347.0
33	438.0
34	622.0
35	698.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.73913983142425	20.01296736546358	12.059649881132483	27.188242921979683
2	15.9066349686622	20.380376053598447	38.88048411497731	24.832504862762047
3	18.460873324686556	24.38391699092088	28.102031993082576	29.05317769130999
4	11.262429744920018	15.045395590142672	35.927367055771725	37.76480760916559
5	13.921314310419369	36.68396022481625	34.15477734543882	15.239948119325552
6	32.1011673151751	37.224383916990924	16.731517509727624	13.942931258106356
7	28.237837837837837	31.28648648648649	21.83783783783784	18.637837837837836
8	28.401470906337877	32.33830845771145	19.900497512437813	19.35972312351287
9	27.319922128487995	12.589227774172615	19.46787800129786	40.62297209604153
10	14.499026184808484	28.21900021640338	31.616533217918196	25.665440380869942
11	37.46484966471988	21.522820679212632	22.020333117023576	18.991996539043914
12	22.902249134948097	24.459342560553633	29.8659169550173	22.77249134948097
13	27.74653979238754	20.155709342560556	25.389273356401382	26.708477508650518
14	21.868916288124595	20.311486048020765	26.88730261734804	30.932295046506596
15	23.544687297121836	27.63471110149318	23.371564596407705	25.449037004977278
16	24.810728963876272	26.47631408176509	23.858966039368376	24.853990914990266
17	22.45295262816353	26.71425481289206	26.368159203980102	24.46463335496431
18	22.44103008006925	25.470677342566546	27.48322873836832	24.60506383899589
19	24.080484638684553	23.929035049762007	27.3691042838598	24.62137602769364
20	24.881003894418	24.469926438771093	26.893119861531805	23.7559498052791
21	24.51319774989182	25.227174383383815	26.027693639117267	24.231934227607095
22	24.756546202120752	25.513958017745075	26.50941354685133	23.22008223328284
23	24.55646906101255	23.474686282994377	25.941151016875814	26.027693639117267
24	23.106880138468195	25.573344872349637	26.93639117265253	24.38338381652964
25	23.464532871972317	24.156574394463668	27.357266435986162	25.021626297577853
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.5
3	3.0
4	3.0
5	3.0
6	5.0
7	7.0
8	7.0
9	7.0
10	9.5
11	12.0
12	12.0
13	12.0
14	17.0
15	22.0
16	22.0
17	22.0
18	28.5
19	35.0
20	35.0
21	35.0
22	66.5
23	98.0
24	98.0
25	98.0
26	136.5
27	175.0
28	175.0
29	175.0
30	209.5
31	244.0
32	244.0
33	244.0
34	283.5
35	323.0
36	323.0
37	323.0
38	374.5
39	426.0
40	426.0
41	426.0
42	469.5
43	513.0
44	513.0
45	513.0
46	524.0
47	535.0
48	535.0
49	535.0
50	569.0
51	603.0
52	603.0
53	603.0
54	536.0
55	469.0
56	469.0
57	469.0
58	454.0
59	439.0
60	439.0
61	439.0
62	378.0
63	317.0
64	317.0
65	317.0
66	266.0
67	215.0
68	215.0
69	215.0
70	156.5
71	98.0
72	98.0
73	98.0
74	65.5
75	33.0
76	33.0
77	33.0
78	33.5
79	34.0
80	34.0
81	34.0
82	24.5
83	15.0
84	15.0
85	15.0
86	9.0
87	3.0
88	3.0
89	3.0
90	3.0
91	3.0
92	3.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	2.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.02161227577263886
4	0.02161227577263886
5	0.02161227577263886
6	0.02161227577263886
7	0.04322455154527772
8	0.08644910309055544
9	0.08644910309055544
10	0.12967365463583316
11	0.08644910309055544
12	0.06483682731791658
13	0.06483682731791658
14	0.08644910309055544
15	0.12967365463583316
16	0.08644910309055544
17	0.08644910309055544
18	0.12967365463583316
19	0.1080613788631943
20	0.1080613788631943
21	0.1080613788631943
22	0.12967365463583316
23	0.1080613788631943
24	0.1080613788631943
25	0.06483682731791658
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4627.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.58698940998488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.70494864612512	88.61033066781933
2	2.987861811391223	5.532742597795548
3	0.700280112044818	1.9451048195374971
4	0.2801120448179272	1.0373892370866653
5	0.11671335200746966	0.5403068943159715
6	0.04668534080298786	0.2593473092716663
7	0.02334267040149393	0.15128593040847202
8	0.04668534080298786	0.34579641236222175
9	0.02334267040149393	0.19451048195374973
>10	0.0700280112044818	1.383185649448887
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	31	0.6699805489518046	No Hit
TATCAACGCAGAGTACTTTTTTTTT	21	0.45385779122541603	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	12	0.2593473092716663	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	9	0.19451048195374973	No Hit
GTATCTGATCGTCTTCGAACCTCCG	8	0.17289820618111088	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8	0.17289820618111088	No Hit
GTCAATCCTGTCCGTGTCCGGGCCG	7	0.15128593040847202	No Hit
GTACATGGGTACCTGGTTGATCCTG	6	0.12967365463583316	No Hit
GTATCAACGCAGAGTACATGGGAAT	6	0.12967365463583316	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5	0.1080613788631943	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	5	0.1080613788631943	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	5	0.1080613788631943	No Hit
GCTTTGAACACTCTAATTTTTTCAA	5	0.1080613788631943	No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG	5	0.1080613788631943	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE