##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064175_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4627
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.91571212448671	32.0	32.0	32.0	21.0	32.0
2	29.64512643181327	32.0	32.0	32.0	21.0	32.0
3	30.522368705424682	32.0	32.0	32.0	32.0	32.0
4	30.949427274692024	32.0	32.0	32.0	32.0	32.0
5	30.623946401556083	32.0	32.0	32.0	32.0	32.0
6	34.322239031770046	36.0	36.0	36.0	32.0	36.0
7	34.26885671061163	36.0	36.0	36.0	32.0	36.0
8	34.174411065485195	36.0	36.0	36.0	32.0	36.0
9	34.502701534471576	36.0	36.0	36.0	32.0	36.0
10	34.02982494056624	36.0	36.0	36.0	32.0	36.0
11	34.75729414307327	36.0	36.0	36.0	32.0	36.0
12	34.2563215906635	36.0	36.0	36.0	32.0	36.0
13	34.51588502269289	36.0	36.0	36.0	32.0	36.0
14	34.482169872487574	36.0	36.0	36.0	32.0	36.0
15	34.33628701102226	36.0	36.0	36.0	32.0	36.0
16	34.26626323751891	36.0	36.0	36.0	32.0	36.0
17	34.325696995893665	36.0	36.0	36.0	32.0	36.0
18	34.36827317916577	36.0	36.0	36.0	32.0	36.0
19	34.3578992867949	36.0	36.0	36.0	32.0	36.0
20	34.3161875945537	36.0	36.0	36.0	32.0	36.0
21	34.40414955694835	36.0	36.0	36.0	32.0	36.0
22	34.3578992867949	36.0	36.0	36.0	32.0	36.0
23	34.25459260860168	36.0	36.0	36.0	32.0	36.0
24	34.24918953965852	36.0	36.0	36.0	32.0	36.0
25	34.27123406094662	36.0	36.0	36.0	32.0	36.0
26	34.02053166198401	36.0	36.0	36.0	32.0	36.0
27	34.07650745623514	36.0	36.0	36.0	32.0	36.0
28	34.05251783012751	36.0	36.0	36.0	32.0	36.0
29	33.977955478711905	36.0	36.0	36.0	32.0	36.0
30	34.13831856494489	36.0	36.0	36.0	32.0	36.0
31	34.06483682731792	36.0	36.0	36.0	32.0	36.0
32	33.72574022044521	36.0	36.0	36.0	32.0	36.0
33	33.9500756429652	36.0	36.0	36.0	32.0	36.0
34	33.702398962610765	36.0	36.0	36.0	32.0	36.0
35	33.8681651177869	36.0	36.0	36.0	32.0	36.0
36	33.61897557812838	36.0	36.0	36.0	27.0	36.0
37	33.66241625243138	36.0	36.0	36.0	27.0	36.0
38	33.546790577047766	36.0	36.0	36.0	21.0	36.0
39	33.67581586341042	36.0	36.0	36.0	32.0	36.0
40	33.64404581802464	36.0	36.0	36.0	27.0	36.0
41	33.646207045601905	36.0	36.0	36.0	27.0	36.0
42	33.39010157769613	36.0	36.0	36.0	21.0	36.0
43	33.386211368057054	36.0	36.0	36.0	21.0	36.0
44	33.465960665658095	36.0	36.0	36.0	21.0	36.0
45	33.215474389453206	36.0	36.0	36.0	21.0	36.0
46	33.25091852172034	36.0	36.0	36.0	21.0	36.0
47	33.30213961530149	36.0	36.0	36.0	21.0	36.0
48	33.11757078020315	36.0	36.0	36.0	21.0	36.0
49	33.27231467473525	36.0	36.0	36.0	21.0	36.0
50	32.25372811757078	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	2.0
24	3.0
25	13.0
26	38.0
27	65.0
28	97.0
29	147.0
30	226.0
31	315.0
32	430.0
33	698.0
34	1098.0
35	1494.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81391830559758	17.916576615517616	13.745407391398315	25.524097687486492
2	19.732007780419277	19.796844607737192	35.24962178517398	25.22152582666955
3	21.914847633455803	24.44348389885455	27.382753403933435	26.25891506375621
4	16.87918737843095	15.92824724443484	35.617030473308844	31.575534903825375
5	18.99719040414956	34.55802896044953	30.7542684244651	15.690512210935811
6	35.984439161443696	36.43829695266911	15.452777177436783	12.1244867084504
7	31.078452561054682	29.93300194510482	19.494272746920252	19.494272746920252
8	27.036956991571216	33.17484331100064	19.34298681651178	20.445212880916362
9	26.064404581802464	13.399610979036092	18.283985303652475	42.25199913550897
10	14.393775664577479	27.015344715798573	31.294575318781064	27.296304300842877
11	37.713421223254805	20.510049708234277	22.34709314890858	19.429435919602334
12	24.335422519991354	21.82839853036525	28.8091636049276	25.027015344715796
13	28.549816295655933	19.796844607737192	25.264750378214828	26.388588718392047
14	23.406094661767884	18.283985303652475	25.86989409984871	32.440025934730926
15	25.486381322957197	25.789018590575015	22.070903588413316	26.653696498054476
16	25.113464447806354	25.6969958936676	23.838340177220662	25.351199481305382
17	24.03285065917441	25.286362653987464	25.264750378214828	25.416036308623298
18	24.659606656580937	23.66544197103955	26.4750378214826	25.19991355089691
19	25.243138102442188	24.746055759671492	26.53987464880052	23.4709314890858
20	25.95634320293927	24.357034795763994	23.622217419494273	26.064404581802464
21	27.598876161659824	23.406094661767884	24.03285065917441	24.96217851739788
22	24.600086467790746	25.183744055339385	25.118893212278426	25.09727626459144
23	24.140912038037605	24.832504862762047	26.53987464880052	24.486708450399828
24	24.184136589582884	25.416036308623298	25.22152582666955	25.17830127512427
25	25.17830127512427	24.918953965852605	25.86989409984871	24.03285065917441
26	24.8	26.46486486486486	24.021621621621623	24.71351351351351
27	25.502485411713856	24.529933001945107	24.724443483898856	25.243138102442188
28	23.95157803718115	24.31906614785992	27.496757457846954	24.232598357111975
29	24.918953965852605	24.227361141128164	25.37281175707802	25.480873135941213
30	24.594769829263022	25.437648584395937	24.529933001945107	25.437648584395937
31	23.98962610762913	25.437648584395937	25.351199481305382	25.22152582666955
32	25.486381322957197	25.03242542153048	23.843493298746218	25.637699956766102
33	23.281452658884564	24.535235624729786	25.961954172070907	26.221357544314746
34	24.31906614785992	24.859489840034588	25.616083009079116	25.205361003026372
35	26.9347168179853	24.96757457846952	24.427150886294854	23.670557717250322
36	23.80025940337224	24.643320363164722	24.96757457846952	26.588845654993516
37	25.33506268914829	24.470384781668827	24.924340683095547	25.27021184608733
38	26.480760916558584	23.994811932555123	24.57846952010376	24.945957630782534
39	25.535135135135135	24.627027027027026	25.210810810810813	24.627027027027026
40	25.03783783783784	24.821621621621624	25.6	24.54054054054054
41	25.421530479896237	25.054042369217466	25.291828793774318	24.232598357111975
42	25.22152582666955	25.848281824076075	25.113464447806354	23.816727901448022
43	24.01123838340177	24.724443483898856	25.6969958936676	25.56732223903177
44	24.29219796844608	25.977955478711905	24.529933001945107	25.19991355089691
45	25.572849113705143	25.205361003026372	24.059662775616083	25.1621271076524
46	25.113464447806354	25.17830127512427	24.897341690079966	24.81089258698941
47	25.156688999351633	25.459260860168577	25.37281175707802	24.01123838340177
48	25.632159066349686	25.04862762048844	24.119299762264966	25.19991355089691
49	23.708666522584828	25.524097687486492	26.49665009725524	24.27058569267344
50	25.243138102442188	25.070239896261075	24.724443483898856	24.96217851739788
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	1.5
4	2.0
5	2.0
6	2.0
7	1.5
8	1.0
9	1.5
10	2.0
11	4.0
12	6.0
13	7.0
14	8.0
15	6.5
16	5.0
17	7.5
18	10.0
19	10.5
20	11.0
21	13.5
22	16.0
23	27.0
24	38.0
25	34.5
26	31.0
27	37.0
28	43.0
29	56.0
30	69.0
31	81.0
32	93.0
33	106.5
34	120.0
35	124.0
36	128.0
37	148.5
38	169.0
39	194.0
40	219.0
41	215.5
42	212.0
43	232.5
44	253.0
45	256.5
46	260.0
47	276.0
48	292.0
49	307.5
50	323.0
51	327.0
52	331.0
53	336.5
54	342.0
55	319.5
56	297.0
57	295.0
58	293.0
59	274.5
60	256.0
61	234.5
62	213.0
63	193.5
64	174.0
65	141.0
66	108.0
67	95.5
68	83.0
69	74.5
70	66.0
71	54.5
72	43.0
73	38.5
74	34.0
75	31.5
76	29.0
77	19.5
78	10.0
79	8.5
80	7.0
81	6.5
82	6.0
83	5.0
84	4.0
85	2.5
86	1.0
87	2.0
88	3.0
89	1.5
90	0.0
91	1.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	4.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.02161227577263886
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.02161227577263886
23	0.0
24	0.0
25	0.0
26	0.04322455154527772
27	0.0
28	0.02161227577263886
29	0.0
30	0.0
31	0.0
32	0.02161227577263886
33	0.02161227577263886
34	0.02161227577263886
35	0.02161227577263886
36	0.02161227577263886
37	0.02161227577263886
38	0.02161227577263886
39	0.04322455154527772
40	0.04322455154527772
41	0.02161227577263886
42	0.0
43	0.0
44	0.0
45	0.02161227577263886
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4627.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.61270801815431
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.99367088607595	92.73827534039334
2	2.2603978300180834	4.322455154527772
3	0.33905967450271246	0.9725524097687486
4	0.2034358047016275	0.7780419278149989
5	0.11301989150090416	0.5403068943159715
6	0.045207956600361664	0.2593473092716663
7	0.0	0.0
8	0.0	0.0
9	0.045207956600361664	0.38902096390749946
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	9	0.19451048195374973	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	9	0.19451048195374973	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	6	0.12967365463583316	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	6	0.12967365463583316	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	5	0.1080613788631943	No Hit
GGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG	5	0.1080613788631943	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	5	0.1080613788631943	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	5	0.1080613788631943	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	5	0.1080613788631943	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.02161227577263886	0.0
30	0.0	0.0	0.0	0.02161227577263886	0.0
31	0.0	0.0	0.0	0.02161227577263886	0.0
32	0.0	0.0	0.0	0.02161227577263886	0.0
33	0.0	0.0	0.0	0.02161227577263886	0.0
34	0.0	0.0	0.0	0.02161227577263886	0.0
35	0.0	0.0	0.0	0.04322455154527772	0.0
36	0.0	0.0	0.0	0.04322455154527772	0.0
37	0.0	0.0	0.0	0.06483682731791658	0.0
38	0.0	0.0	0.0	0.06483682731791658	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE