FastQCFastQC Report
Thu 2 Feb 2017
SRR4064174_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064174_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064222
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30660.2880977841089547No Hit
TATCAACGCAGAGTACTTTTTTTTT21910.20587809686324846No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19400.1822927922933373No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12710.1194299685591916No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11270.10589895717246967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA651.9105937E-716.0776799
GTACTAA551.1286453E-515.5439921
GTCGATG400.005274360514.2513412
ACGCGCG400.005274360514.2513413
CGATCAG801.9966537E-613.06311410
GGGTAGG1550.012.8697571
ATCCCCG1051.9881554E-812.66785812
GACCATA1155.315087E-912.39130411
CGACCAT1001.4398574E-712.35058110
TAGAGTG1405.2750693E-1112.2125625
ATACGAA1209.997166E-911.8749996
GTATAAG802.8671253E-511.8738831
AATACGA1201.0017175E-811.8733245
GCGTTAT658.0260966E-411.6912081
GTCCTAT1552.5465852E-1111.6440661
TCGCCAG907.442546E-611.6127517
GTATTAT1001.929684E-611.3989281
CAATACG1251.8335413E-811.3978564
CCTATTC1001.9329618E-611.397323
GGCGAGA3600.011.3482919
Produced by FastQC (version 0.11.5)