FastQCFastQC Report
Thu 2 Feb 2017
SRR4064174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1064222
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA4250.021.2205831
GTGTATA3350.020.3553831
GGGTATA654.936249E-420.3048731
GCTATAC1051.7946586E-618.8545253
ATACGAA2400.018.3316540
GTCCTAC7300.018.0796811
TAGGACG6600.017.9975014
ATTTCGT3350.017.72971542
GTATCAA24650.017.2227611
TACGAAT2300.017.21580941
ATTCGTA2700.017.11195224
ATACCGT1702.4701876E-916.8211946
CGCGCGT2251.8189894E-1216.6199157
GTAGGAC8350.016.5964983
CGTCGTA1602.1305823E-816.49848610
TATGGCG8150.016.46397216
TAGGACC6300.016.410424
TAGCGCG2301.8189894E-1216.2586124
TATTGCG2850.016.21132329
CTATACT953.349383E-416.2082774
Produced by FastQC (version 0.11.5)