##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064174_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1064222
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30811334477205	32.0	32.0	32.0	32.0	32.0
2	31.415752540353424	32.0	32.0	32.0	32.0	32.0
3	31.47484359466352	32.0	32.0	32.0	32.0	32.0
4	31.560614232744673	32.0	32.0	32.0	32.0	32.0
5	31.52005690542011	32.0	32.0	32.0	32.0	32.0
6	35.08883108975383	36.0	36.0	36.0	36.0	36.0
7	35.11436053755701	36.0	36.0	36.0	36.0	36.0
8	35.06384570136682	36.0	36.0	36.0	36.0	36.0
9	35.1636726171795	36.0	36.0	36.0	36.0	36.0
10	35.02362664932693	36.0	36.0	36.0	36.0	36.0
11	35.16531043334943	36.0	36.0	36.0	36.0	36.0
12	35.073023297770575	36.0	36.0	36.0	36.0	36.0
13	35.1046802264941	36.0	36.0	36.0	36.0	36.0
14	35.05727658326928	36.0	36.0	36.0	36.0	36.0
15	35.0338218905454	36.0	36.0	36.0	36.0	36.0
16	35.046120076450215	36.0	36.0	36.0	36.0	36.0
17	35.01695886760469	36.0	36.0	36.0	36.0	36.0
18	35.00193286739045	36.0	36.0	36.0	36.0	36.0
19	35.002451556160274	36.0	36.0	36.0	36.0	36.0
20	34.99409991524325	36.0	36.0	36.0	36.0	36.0
21	34.987731883009374	36.0	36.0	36.0	36.0	36.0
22	34.975464705672316	36.0	36.0	36.0	36.0	36.0
23	34.91962109409503	36.0	36.0	36.0	32.0	36.0
24	34.89453986104403	36.0	36.0	36.0	32.0	36.0
25	34.868560319181526	36.0	36.0	36.0	32.0	36.0
26	34.81237091509102	36.0	36.0	36.0	32.0	36.0
27	34.808054146597236	36.0	36.0	36.0	32.0	36.0
28	34.77588604633244	36.0	36.0	36.0	32.0	36.0
29	34.75278278404318	36.0	36.0	36.0	32.0	36.0
30	34.73189146625422	36.0	36.0	36.0	32.0	36.0
31	34.71662773368715	36.0	36.0	36.0	32.0	36.0
32	34.671902103132616	36.0	36.0	36.0	32.0	36.0
33	34.65128234522496	36.0	36.0	36.0	32.0	36.0
34	34.64081742343233	36.0	36.0	36.0	32.0	36.0
35	34.59975268318077	36.0	36.0	36.0	32.0	36.0
36	34.562327221200086	36.0	36.0	36.0	32.0	36.0
37	34.547373574310626	36.0	36.0	36.0	32.0	36.0
38	34.510165172304276	36.0	36.0	36.0	32.0	36.0
39	34.483066503041655	36.0	36.0	36.0	32.0	36.0
40	34.4744620953147	36.0	36.0	36.0	32.0	36.0
41	34.46333377810269	36.0	36.0	36.0	32.0	36.0
42	34.39179889158465	36.0	36.0	36.0	32.0	36.0
43	34.38184608098686	36.0	36.0	36.0	32.0	36.0
44	34.309017291504965	36.0	36.0	36.0	32.0	36.0
45	34.27299567195566	36.0	36.0	36.0	32.0	36.0
46	34.23580888198139	36.0	36.0	36.0	32.0	36.0
47	34.20697937084556	36.0	36.0	36.0	32.0	36.0
48	34.15227649870046	36.0	36.0	36.0	32.0	36.0
49	34.12753823920197	36.0	36.0	36.0	32.0	36.0
50	33.54935154507236	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	5.0
20	8.0
21	34.0
22	122.0
23	365.0
24	906.0
25	2118.0
26	4275.0
27	8044.0
28	12679.0
29	19570.0
30	27396.0
31	39142.0
32	55395.0
33	87351.0
34	178438.0
35	628372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.54309879008575	18.073910489839072	11.879149536003759	26.50384118407142
2	15.571231109386318	20.610607403175475	37.747493701954205	26.070667785484
3	18.20181579338068	24.729143840829604	28.93401539910056	28.135024966689155
4	12.307770371219538	16.245388650112478	35.967025676973414	35.47981530169457
5	13.910349532334418	37.384399119732535	33.71928037571108	14.985970972221963
6	33.8477946289301	35.94482343876266	16.860799458758528	13.346582473548702
7	29.56291074606614	30.6959450189904	21.3004429526922	18.44070128225126
8	27.49379358817991	34.06638840392324	19.534082174583876	18.905735833312974
9	27.500664814270152	13.964200573760557	18.567910190404685	39.9672244215646
10	15.579285252507027	27.526479785906517	32.32798475485995	24.566250206726505
11	36.988147209886655	20.91537292031174	22.99322885638523	19.103251013416372
12	24.764663763763576	23.575626138155386	29.709402737398776	21.950307360682263
13	29.97909257058019	19.871924376183383	25.205808976569582	24.94317407666684
14	23.21803762379959	19.922384469376635	25.79466651632181	31.064911390501965
15	24.63025348274485	28.262644771771562	22.492729109403996	24.614372636079594
16	24.768914610460087	25.81165307451394	25.016326557459617	24.40310575756636
17	23.507031427653253	26.298366318305767	25.920343687689222	24.274258566351758
18	24.12783459043674	25.173765925998314	27.36119163442863	23.337207849136316
19	25.482231117626053	24.76856289113153	25.939185506756125	23.81002048448629
20	25.71926323089153	24.671541626026343	25.718793400575446	23.890401742506675
21	26.104327856405902	24.336839494015347	25.070614965674455	24.4882176839043
22	25.493155074284523	24.913857016272026	25.470885221932704	24.122102687510747
23	24.081081512817455	24.787049209133887	26.118831474526242	25.013037803522415
24	24.756557650474765	24.977565623487735	26.109479757379855	24.15639696865765
25	24.595449185072425	24.820593776575002	26.156708528901856	24.427248509450717
26	23.99579392091895	25.69514008047556	26.431767737230942	23.87729826137455
27	24.524488121717507	25.2672272919057	25.691408037633117	24.516876548743667
28	23.955353753284196	25.336688630204442	25.994749048100523	24.713208568410845
29	24.288217854810444	25.22404258618016	25.698109838892307	24.789629720117084
30	23.99751922832966	25.411559080423046	26.11247104591777	24.478450645329524
31	24.889677414504696	24.880186289176734	25.55696930513686	24.67316699118171
32	24.342767363561446	25.462954697297587	25.43664377930026	24.757634159840705
33	24.037253481354934	25.259897629042005	26.081947510216892	24.62090137938617
34	24.6177164566335	25.28159729824459	25.993495565200803	24.107190679921107
35	24.745040589594517	25.274175921758257	26.092827495867745	23.887955992779478
36	24.151829218441506	25.592800967539137	25.89106915142044	24.364300662598918
37	24.632605482327758	25.390933702192196	25.373737411704568	24.602723403775478
38	24.316918323391107	25.60950168585573	25.62481910410958	24.448760886643587
39	24.916907619691088	25.08107203970768	25.409306910410578	24.592713430190656
40	25.305793371352536	25.10714631380033	25.84995851339834	23.73710180144879
41	24.485991144388564	25.272133390466312	26.201953797463595	24.03992166768153
42	24.980478275209805	25.871289110401346	25.646894988625245	23.5013376257636
43	24.23438009477515	25.35362144252553	25.949090540056734	24.46290792264258
44	23.823538255679342	25.76432426743644	25.526494820561396	24.885642656322823
45	24.01057689958523	25.88803295602543	25.626523436252	24.474866708137334
46	24.059465762834183	25.205575220656844	25.654547180112175	25.0804118363968
47	24.66599386206754	25.225943946731917	25.78965271630762	24.318409474892924
48	24.597045107080334	25.725497344956448	25.142618734618743	24.53483881334447
49	24.424271012651566	25.709066580468626	25.136344501492182	24.73031790538763
50	24.22409799542009	26.290037802582756	25.064249356096944	24.421614845900212
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	234.0
1	150.5
2	67.0
3	170.5
4	274.0
5	298.0
6	322.0
7	304.5
8	287.0
9	377.5
10	468.0
11	743.5
12	1019.0
13	1682.5
14	2346.0
15	3013.0
16	3680.0
17	3743.5
18	3807.0
19	3796.0
20	3785.0
21	4109.5
22	4434.0
23	5054.5
24	5675.0
25	6394.0
26	7113.0
27	8731.5
28	10350.0
29	12675.0
30	15000.0
31	18053.0
32	21106.0
33	24954.5
34	28803.0
35	33472.5
36	38142.0
37	41778.0
38	45414.0
39	47083.5
40	48753.0
41	51471.5
42	54190.0
43	56037.0
44	57884.0
45	62855.5
46	67827.0
47	71349.0
48	74871.0
49	77379.0
50	79887.0
51	78479.0
52	77071.0
53	76115.5
54	75160.0
55	74676.0
56	74192.0
57	71219.5
58	68247.0
59	62033.5
60	55820.0
61	49241.5
62	42663.0
63	36951.0
64	31239.0
65	26415.0
66	21591.0
67	17986.0
68	14381.0
69	12647.0
70	10913.0
71	8544.5
72	6176.0
73	5249.5
74	4323.0
75	3354.5
76	2386.0
77	2042.5
78	1699.0
79	1423.5
80	1148.0
81	916.5
82	685.0
83	566.0
84	447.0
85	303.5
86	160.0
87	119.0
88	78.0
89	59.0
90	40.0
91	32.5
92	25.0
93	17.0
94	9.0
95	13.5
96	18.0
97	12.5
98	7.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009114639614666865
2	2.818960705567072E-4
3	3.758614274089429E-4
4	0.0
5	0.0
6	1.8793071370447144E-4
7	0.0
8	0.0
9	0.0014094803527835357
10	0.0013155149959313
11	0.0
12	0.0
13	6.5775749796565E-4
14	1.8793071370447144E-4
15	0.004416371772055078
16	4.698267842611786E-4
17	0.0
18	2.818960705567072E-4
19	1.8793071370447144E-4
20	7.517228548178858E-4
21	0.0
22	2.818960705567072E-4
23	8.456882116701214E-4
24	6.5775749796565E-4
25	0.0015974110664880071
26	0.004510337128907315
27	0.004980163913168493
28	0.0031948221329760143
29	0.0025370646350103645
30	0.0034767182035327217
31	0.0065775749796565
32	0.002349133921305893
33	0.004980163913168493
34	0.002349133921305893
35	0.003100856776123779
36	0.007423263191326621
37	0.003664648917237193
38	0.006953436407065443
39	0.004228441058350608
40	0.0021612032076014213
41	0.0035706835603849575
42	0.0021612032076014213
43	0.0017853417801924787
44	0.0013155149959313
45	0.0015034457096357715
46	0.0012215496390790644
47	0.0018793071370447143
48	0.00197327249389695
49	5.637921411134144E-4
50	8.456882116701214E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1064222.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.50496092057027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.83329528374855	51.49643592438547
2	12.952725022486678	16.710300427807915
3	3.539014014284573	6.848518820663306
4	1.4013935602026106	3.615873473408329
5	0.6662057807276699	2.1486788925448175
6	0.40328442209978616	1.560830753245288
7	0.2498013323190048	1.1279397625400653
8	0.1740078915686291	0.8979497796404198
9	0.12457788196516995	0.7232302266957633
>10	0.5627284893051708	6.3700731194863085
>50	0.048549677312563745	2.236583755007213
>100	0.0416314435274652	5.156033089783183
>500	0.0026386109545885736	1.0093067343062245
>1k	1.4658949747714298E-4	0.09824524048568177
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.698267842611786E-4
2	0.0	0.0	0.0	0.0	4.698267842611786E-4
3	0.0	0.0	0.0	0.0	4.698267842611786E-4
4	0.0	0.0	0.0	0.0	4.698267842611786E-4
5	0.0	0.0	0.0	0.0	4.698267842611786E-4
6	0.0	0.0	0.0	0.0	5.637921411134143E-4
7	0.0	0.0	0.0	0.0	5.637921411134143E-4
8	0.0	0.0	0.0	0.0	5.637921411134143E-4
9	0.0	0.0	0.0	0.0	5.637921411134143E-4
10	0.0	0.0	0.0	0.0	6.577574979656501E-4
11	0.0	0.0	0.0	0.0	6.577574979656501E-4
12	0.0	0.0	0.0	0.0	0.0011275842822268285
13	0.0	0.0	0.0	0.0	0.0013155149959313002
14	0.0	0.0	0.0	0.0	0.0013155149959313002
15	0.0	0.0	0.0	0.0	0.0017853417801924787
16	0.0	0.0	0.0	0.0	0.0018793071370447143
17	0.0	0.0	0.0	0.0	0.0018793071370447143
18	0.0	0.0	0.0	9.396535685223572E-5	0.00197327249389695
19	0.0	0.0	0.0	9.396535685223572E-5	0.00197327249389695
20	0.0	0.0	0.0	9.396535685223572E-5	0.002067237850749186
21	0.0	0.0	0.0	1.8793071370447144E-4	0.002349133921305893
22	0.0	0.0	0.0	3.758614274089429E-4	0.0024430992781581287
23	0.0	0.0	0.0	0.0012215496390790644	0.0024430992781581287
24	0.0	0.0	0.0	0.002349133921305893	0.0024430992781581287
25	0.0	0.0	0.0	0.0029129260624193073	0.0024430992781581287
26	0.0	0.0	0.0	0.0037586142740894286	0.0024430992781581287
27	0.0	0.0	0.0	0.005168094626872965	0.0024430992781581287
28	0.0	0.0	0.0	0.007611193905031093	0.0024430992781581287
29	0.0	0.0	0.0	0.01381290745727865	0.0024430992781581287
30	0.0	0.0	0.0	0.02696805741659165	0.0024430992781581287
31	0.0	0.0	0.0	0.05562749125652355	0.0024430992781581287
32	0.0	0.0	0.0	0.08597830151979569	0.0025370646350103645
33	0.0	0.0	0.0	0.11670497321047676	0.0025370646350103645
34	0.0	0.0	0.0	0.14827733311282795	0.0028189607055670715
35	0.0	0.0	0.0	0.18482985692834766	0.0028189607055670715
36	0.0	0.0	0.0	0.23528925355799823	0.0028189607055670715
37	0.0	0.0	0.0	0.3156296336666598	0.0028189607055670715
38	0.0	0.0	0.0	0.42425358618784426	0.0029129260624193073
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAA	425	0.0	21.220583	1
GTGTATA	335	0.0	20.355383	1
GGGTATA	65	4.936249E-4	20.304873	1
GCTATAC	105	1.7946586E-6	18.854525	3
ATACGAA	240	0.0	18.33165	40
GTCCTAC	730	0.0	18.079681	1
TAGGACG	660	0.0	17.997501	4
ATTTCGT	335	0.0	17.729715	42
GTATCAA	2465	0.0	17.222761	1
TACGAAT	230	0.0	17.215809	41
ATTCGTA	270	0.0	17.111952	24
ATACCGT	170	2.4701876E-9	16.821194	6
CGCGCGT	225	1.8189894E-12	16.619915	7
GTAGGAC	835	0.0	16.596498	3
CGTCGTA	160	2.1305823E-8	16.498486	10
TATGGCG	815	0.0	16.463972	16
TAGGACC	630	0.0	16.41042	4
TAGCGCG	230	1.8189894E-12	16.258612	4
TATTGCG	285	0.0	16.211323	29
CTATACT	95	3.349383E-4	16.208277	4
>>END_MODULE