FastQCFastQC Report
Thu 2 Feb 2017
SRR4064173_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064173_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920250
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33060.35925020374898126No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29590.3215430589513719No Hit
TATCAACGCAGAGTACTTTTTTTTT27610.300027166530834No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17760.1929910350448248No Hit
GTACATGGAAGCAGTGGTATCAACG17540.19060038033143167No Hit
TCCATGTACTCTGCGTTGATACCAC16270.17679978266775334No Hit
ACGCAGAGTACTTTTTTTTTTTTTT15600.16951915240423798No Hit
GTACATGGGGTGGTATCAACGCAAA14180.1540885628905189No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13060.1419179570768813No Hit
GGTATCAACGCAGAGTACTTTTTTT12840.13952730236348818No Hit
GAATAGGACCGCGGTTCTATTTTGT12480.13561532192339038No Hit
GTCCTATTCCATTATTCCTAGCTGC12480.13561532192339038No Hit
GATTAAGAGGGACGGCCGGGGGCAT12080.13126867698994837No Hit
GTATCTGATCGTCTTCGAACCTCCG12070.13116001086661233No Hit
CTATTGGAGCTGGAATTACCGCGGC11890.12920402064656342No Hit
GAACTACGACGGTATCTGATCGTCT11440.12431404509644119No Hit
GAATAACGCCGCCGCATCGCCAGTC11360.12344471610975277No Hit
GCGCAAGACGGACCAGAGCGAAAGC10610.11529475685954904No Hit
ATCAGATACCGTCGTAGTTCCGACC10510.11420809562618854No Hit
GTCTTGCGCCGGTCCAAGAATTTCA10330.11225210540613964No Hit
CTTTAATATACGCTATTGGAGCTGG10000.10866612333604998No Hit
GTACATGGGGAATAATTGCAATCCC9620.10453681064928008No Hit
TCGTAGTTCCGACCATAAACGATGC9590.10421081227927192No Hit
GCTTTGAACACTCTAATTTTTTCAA9560.10388481390926378No Hit
CTCTTAATCATGGCCTCAGTTCCGA9300.1010594947025265No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG9210.10008149959250204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCCC504.55834E-617.099955
TCGGCGT753.274181E-916.46751613
CTCGTAG1001.8189894E-1216.15083110
GTATTAA508.768777E-515.1875661
TATTAAC456.800684E-414.7656882
AACCGCG1500.014.5674177
GTCGGCG1006.566552E-1014.25150912
CGTCTTA759.631058E-713.93708415
ATAATTC500.001503073413.29493
GCTCGTA1153.7471182E-1013.219519
TAGAACC2050.012.9734544
CCGTCGT1700.012.8550029
CGCGGTC1700.012.85360410
GCGCCGA1207.348717E-1012.67007619
CGGCGTC905.382881E-712.66938714
CTAGACA604.094931E-412.6645654
CGTCGTA1750.012.48635910
CGACCCG1600.012.4687145
AGAACCG2000.012.3499645
CGAACGA853.9265306E-612.29742716
Produced by FastQC (version 0.11.5)