##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064173_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	920250
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.169388753056236	32.0	32.0	32.0	32.0	32.0
2	30.83150230915512	32.0	32.0	32.0	32.0	32.0
3	30.83921108394458	32.0	32.0	32.0	32.0	32.0
4	30.85664330345015	32.0	32.0	32.0	32.0	32.0
5	30.811950013583264	32.0	32.0	32.0	32.0	32.0
6	34.32087150230915	36.0	36.0	36.0	32.0	36.0
7	34.20716870415648	36.0	36.0	36.0	32.0	36.0
8	34.12460092366205	36.0	36.0	36.0	32.0	36.0
9	34.34731323010052	36.0	36.0	36.0	32.0	36.0
10	33.981951643575115	36.0	36.0	36.0	32.0	36.0
11	34.27885574572127	36.0	36.0	36.0	32.0	36.0
12	34.10493561532192	36.0	36.0	36.0	32.0	36.0
13	34.19675957620212	36.0	36.0	36.0	32.0	36.0
14	34.067713121434394	36.0	36.0	36.0	32.0	36.0
15	34.08874870958979	36.0	36.0	36.0	32.0	36.0
16	34.09571312143439	36.0	36.0	36.0	32.0	36.0
17	33.95907525129041	36.0	36.0	36.0	32.0	36.0
18	34.01618038576474	36.0	36.0	36.0	32.0	36.0
19	34.015301276826946	36.0	36.0	36.0	32.0	36.0
20	33.90813583265417	36.0	36.0	36.0	32.0	36.0
21	33.84732953001902	36.0	36.0	36.0	32.0	36.0
22	33.89977180114099	36.0	36.0	36.0	32.0	36.0
23	33.78625808204292	36.0	36.0	36.0	32.0	36.0
24	33.75227492529204	36.0	36.0	36.0	32.0	36.0
25	33.182834012496606	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	9.0
5	15.0
6	40.0
7	13.0
8	47.0
9	54.0
10	75.0
11	28.0
12	53.0
13	49.0
14	174.0
15	349.0
16	532.0
17	725.0
18	933.0
19	1193.0
20	1561.0
21	2052.0
22	2628.0
23	3587.0
24	4784.0
25	6754.0
26	9188.0
27	12497.0
28	17363.0
29	24346.0
30	33553.0
31	49140.0
32	74958.0
33	112803.0
34	235712.0
35	325032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.58532096528198	17.533267311449	11.68359066286769	25.197821060401328
2	16.071257802583762	20.151313616052022	38.25917618625354	25.518252395110668
3	18.7783963409267	25.063652357609435	28.48365576978874	27.67429553167513
4	12.052018504451697	15.587729335989245	36.03217088254759	36.32808127701147
5	14.187905634213033	37.14522628574906	33.48468316517491	15.182184914863003
6	33.50120907485396	35.67884798261105	17.354707240864013	13.465235701670968
7	29.590902865788575	30.612069658900943	21.26092088701841	18.53610658829207
8	28.125669201979697	32.857069870937096	19.780007152648167	19.237253774435047
9	27.58636809873311	13.970557058880445	18.73456887432554	39.7085059680609
10	16.013551567497984	26.570487939061437	31.365724884913877	26.0502356085267
11	37.023003677022196	20.951742073527395	22.917133270853675	19.10812097859673
12	24.998994216472887	23.558929672353784	29.250903302340703	22.191172808832626
13	29.77905118140202	19.22939750127763	25.790120369262887	25.201430948057457
14	23.684840049583542	20.08176934954222	25.382097731770436	30.851292869103798
15	24.983554866943926	27.606947728940717	23.022968822202287	24.386528581913073
16	25.33808285789157	25.404519659747887	24.91847628119186	24.338921201168677
17	23.344032427824605	25.747196924227794	26.146238354948988	24.762532292998618
18	24.58661980331748	24.389241294411523	27.629185049692623	23.394953852578375
19	25.4362251167363	24.653705874548987	25.8696772706216	24.040391738093113
20	25.51378391604589	24.016421075526072	25.834919253982925	24.63487575444511
21	27.02440244459427	23.94451814959003	24.41527653928968	24.615802866526025
22	25.55318119287256	24.427189533954294	25.327961111201734	24.691668161971407
23	23.88177219897924	24.11069772803647	25.98147062629892	26.026059446685363
24	24.49782556921802	25.23775190283084	25.734298587027798	24.530123940923342
25	24.566148371928133	24.484804590051265	25.850466639262077	25.09858039875853
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	203.0
1	203.0
2	499.5
3	796.0
4	796.0
5	796.0
6	2236.5
7	3677.0
8	3677.0
9	3677.0
10	3281.0
11	2885.0
12	2885.0
13	2885.0
14	2517.0
15	2149.0
16	2149.0
17	2149.0
18	3940.5
19	5732.0
20	5732.0
21	5732.0
22	9954.0
23	14176.0
24	14176.0
25	14176.0
26	22262.5
27	30349.0
28	30349.0
29	30349.0
30	37851.0
31	45353.0
32	45353.0
33	45353.0
34	53024.5
35	60696.0
36	60696.0
37	60696.0
38	66761.5
39	72827.0
40	72827.0
41	72827.0
42	84036.5
43	95246.0
44	95246.0
45	95246.0
46	110309.5
47	125373.0
48	125373.0
49	125373.0
50	123889.0
51	122405.0
52	122405.0
53	122405.0
54	109983.0
55	97561.0
56	97561.0
57	97561.0
58	90333.5
59	83106.0
60	83106.0
61	83106.0
62	74035.5
63	64965.0
64	64965.0
65	64965.0
66	54128.5
67	43292.0
68	43292.0
69	43292.0
70	32692.5
71	22093.0
72	22093.0
73	22093.0
74	17307.5
75	12522.0
76	12522.0
77	12522.0
78	10547.0
79	8572.0
80	8572.0
81	8572.0
82	5832.0
83	3092.0
84	3092.0
85	3092.0
86	2431.5
87	1771.0
88	1771.0
89	1771.0
90	1230.5
91	690.0
92	690.0
93	690.0
94	444.0
95	198.0
96	198.0
97	198.0
98	359.5
99	521.0
100	521.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038359141537625645
2	0.004563977180114099
3	0.0018473240967128498
4	0.004237978810105949
5	0.009345286606900299
6	0.013583265417006248
7	0.024341211627275195
8	0.03379516435751155
9	0.04748709589785385
10	0.0547677261613692
11	0.0525944036946482
12	0.06183102417821244
13	0.063026351534909
14	0.06411301276826949
15	0.05704971475142624
16	0.06291768541157294
17	0.0593317033414833
18	0.07574028796522683
19	0.07128497690844879
20	0.07606628633523499
21	0.07280630263515349
22	0.07660961695191523
23	0.08008693289866885
24	0.07595762021189893
25	0.07563162184189079
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	920250.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.749582314521824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.10207314644155	36.33842206011772
2	14.067650855218861	13.434489049664528
3	4.422910646183109	6.3357640790908585
4	1.8319203514393954	3.498937264588127
5	0.9914772685140889	2.3671312722945372
6	0.5484430833913141	1.5712756893134228
7	0.36578984823041055	1.2226418727526105
8	0.25919026081066526	0.9900981354960994
9	0.18701923242811752	0.8037081209902579
>10	0.9627038586025741	8.583886777953177
>50	0.1138677901776001	3.8555634721653376
>100	0.12778579006148505	12.855448362356922
>500	0.014604090286393322	4.734824285195809
>1k	0.0045637782144979135	3.4078095580205763
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3306	0.35925020374898126	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2959	0.3215430589513719	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2761	0.300027166530834	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1776	0.1929910350448248	No Hit
GTACATGGAAGCAGTGGTATCAACG	1754	0.19060038033143167	No Hit
TCCATGTACTCTGCGTTGATACCAC	1627	0.17679978266775334	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1560	0.16951915240423798	No Hit
GTACATGGGGTGGTATCAACGCAAA	1418	0.1540885628905189	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1306	0.1419179570768813	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1284	0.13952730236348818	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1248	0.13561532192339038	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1248	0.13561532192339038	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1208	0.13126867698994837	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1207	0.13116001086661233	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1189	0.12920402064656342	No Hit
GAACTACGACGGTATCTGATCGTCT	1144	0.12431404509644119	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1136	0.12344471610975277	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1061	0.11529475685954904	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1051	0.11420809562618854	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1033	0.11225210540613964	No Hit
CTTTAATATACGCTATTGGAGCTGG	1000	0.10866612333604998	No Hit
GTACATGGGGAATAATTGCAATCCC	962	0.10453681064928008	No Hit
TCGTAGTTCCGACCATAAACGATGC	959	0.10421081227927192	No Hit
GCTTTGAACACTCTAATTTTTTCAA	956	0.10388481390926378	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	930	0.1010594947025265	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	921	0.10008149959250204	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.0866612333604998E-4	0.0
5	0.0	0.0	0.0	1.0866612333604998E-4	0.0
6	0.0	0.0	0.0	1.0866612333604998E-4	0.0
7	0.0	0.0	0.0	1.0866612333604998E-4	0.0
8	0.0	0.0	0.0	1.0866612333604998E-4	0.0
9	0.0	0.0	0.0	1.0866612333604998E-4	0.0
10	0.0	0.0	0.0	1.0866612333604998E-4	0.0
11	0.0	0.0	0.0	2.1733224667209997E-4	0.0
12	0.0	0.0	0.0	3.2599837000814997E-4	1.0866612333604998E-4
13	0.0	0.0	0.0	3.2599837000814997E-4	2.1733224667209997E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCCC	50	4.55834E-6	17.09995	5
TCGGCGT	75	3.274181E-9	16.467516	13
CTCGTAG	100	1.8189894E-12	16.150831	10
GTATTAA	50	8.768777E-5	15.187566	1
TATTAAC	45	6.800684E-4	14.765688	2
AACCGCG	150	0.0	14.567417	7
GTCGGCG	100	6.566552E-10	14.251509	12
CGTCTTA	75	9.631058E-7	13.937084	15
ATAATTC	50	0.0015030734	13.2949	3
GCTCGTA	115	3.7471182E-10	13.21951	9
TAGAACC	205	0.0	12.973454	4
CCGTCGT	170	0.0	12.855002	9
CGCGGTC	170	0.0	12.853604	10
GCGCCGA	120	7.348717E-10	12.670076	19
CGGCGTC	90	5.382881E-7	12.669387	14
CTAGACA	60	4.094931E-4	12.664565	4
CGTCGTA	175	0.0	12.486359	10
CGACCCG	160	0.0	12.468714	5
AGAACCG	200	0.0	12.349964	5
CGAACGA	85	3.9265306E-6	12.297427	16
>>END_MODULE