FastQCFastQC Report
Thu 2 Feb 2017
SRR4064173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920250
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12800.13909263787014398No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12490.13572398804672642No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12420.13496332518337406No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12110.13159467535995653No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC11990.13029068187992393No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG11300.12279271936973647No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11300.12279271936973647No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA10980.11931540342298289No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC10640.1156207552295572No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10560.11475142624286878No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG10560.11475142624286878No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10090.10964411844607443No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT9970.10834012496604183No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC9640.1047541428959522No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC9400.102146155935887No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9300.1010594947025265No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9240.1004074979625102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC551.5932015E-424.000031
GATATAC3800.023.7368721
GTATATA803.6139536E-622.0000271
ACTGTTC4550.021.274758
GGGTTAG654.930891E-420.3077181
ATATACA4800.020.1655962
TTAGGAC2950.020.135623
GGCGTTA2850.020.0691142
GTATAGA1101.2647979E-720.0000251
TAAGACT1001.1228258E-619.8000244
ATACACT4950.019.555584
GTCCTAA2300.019.1304591
TTAAGAC1051.7917191E-618.8571663
CGCAATA3200.018.56252336
ATTTCGT1901.8189894E-1218.52533142
TATACAC5950.018.4874173
ATACGAA3350.018.38708340
GTGTTAG851.4303486E-418.117671
TTAACGG851.4303486E-418.1176735
TATTATG851.4309441E-418.1166862
Produced by FastQC (version 0.11.5)