FastQCFastQC Report
Thu 2 Feb 2017
SRR4064172_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064172_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614007
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38020.2355627949568992No Hit
TATCAACGCAGAGTACTTTTTTTTT26270.16276261503202896No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17460.10817796948836032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT1150.015.6905052
TAGGACG903.6547135E-813.7177234
GCGTTAT1301.4551915E-1113.14994051
CGAACGA1450.013.10605816
GCGCAAA801.997154E-613.06388611
TTAGAAC802.0090065E-613.0570033
GTTATTC1501.8189894E-1212.6613353
GTATTAG2550.012.2904681
CGGTCCA2900.012.12122410
AAGACGG3150.012.0606585
CTATACT951.043969E-611.9960664
CGACCAT2350.011.7239210
AGGCCCG2850.011.6671810
CGCATCG2400.011.48109713
GTATCAA23800.011.4524811
GCTCGTA1251.8293576E-811.4008559
CACTCTA3200.011.2820979
CGACCCG1604.5474735E-1111.27865
GTCTTAA1553.1468517E-1011.0289821
ACTCTAA3200.010.98485910