FastQCFastQC Report
Thu 2 Feb 2017
SRR4064172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614007
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16460.10198220949475437No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16310.10105284549571346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG609.761376E-625.6647175
GTATCAA26150.022.2955251
ATACCGT3850.018.855716
CGTTTAT5600.018.07453331
GGTATCA14000.017.4436931
AACGCAG33600.017.4153426
TCGTTTA5600.017.28814930
TACACCG1154.2777738E-617.2160845
TTATACT2055.456968E-1217.1694264
CATCGTT5700.016.98432428
TAATAGT1602.1325832E-816.4987454
TACCGTC4000.016.4987457
GTCTAGA2151.2732926E-1116.3728771
GTATAGT1351.0854546E-616.2970771
ATCGTTT5950.016.27069529
TGGTCGG6150.016.09783437
GTATTAG3300.016.0007671
GTATAGG2201.8189894E-1116.0007671
TACTATA1105.628266E-515.9982872
TCAACGC36800.015.7814094
Produced by FastQC (version 0.11.5)