##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064172_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1614007
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.287135681567676	32.0	32.0	32.0	32.0	32.0
2	31.291499355331172	32.0	32.0	32.0	32.0	32.0
3	31.4013576149298	32.0	32.0	32.0	32.0	32.0
4	31.500606874691375	32.0	32.0	32.0	32.0	32.0
5	31.426446725447907	32.0	32.0	32.0	32.0	32.0
6	35.00579737262602	36.0	36.0	36.0	36.0	36.0
7	35.0360512686748	36.0	36.0	36.0	36.0	36.0
8	34.96633657722674	36.0	36.0	36.0	36.0	36.0
9	35.10729755199327	36.0	36.0	36.0	36.0	36.0
10	34.933319372220815	36.0	36.0	36.0	32.0	36.0
11	35.09900700554582	36.0	36.0	36.0	36.0	36.0
12	34.99877323952126	36.0	36.0	36.0	36.0	36.0
13	35.0442879120103	36.0	36.0	36.0	36.0	36.0
14	34.98262832812993	36.0	36.0	36.0	36.0	36.0
15	34.94850084293315	36.0	36.0	36.0	36.0	36.0
16	34.9623991717508	36.0	36.0	36.0	36.0	36.0
17	34.92697243568337	36.0	36.0	36.0	32.0	36.0
18	34.92267877400779	36.0	36.0	36.0	32.0	36.0
19	34.92538260366901	36.0	36.0	36.0	32.0	36.0
20	34.91981075670675	36.0	36.0	36.0	32.0	36.0
21	34.91375192300901	36.0	36.0	36.0	32.0	36.0
22	34.902841809236264	36.0	36.0	36.0	32.0	36.0
23	34.8443755200566	36.0	36.0	36.0	32.0	36.0
24	34.812591271289406	36.0	36.0	36.0	32.0	36.0
25	34.784038111358875	36.0	36.0	36.0	32.0	36.0
26	34.72707119609766	36.0	36.0	36.0	32.0	36.0
27	34.72003714977692	36.0	36.0	36.0	32.0	36.0
28	34.697822871895845	36.0	36.0	36.0	32.0	36.0
29	34.66600702475268	36.0	36.0	36.0	32.0	36.0
30	34.641738852433726	36.0	36.0	36.0	32.0	36.0
31	34.645339828142006	36.0	36.0	36.0	32.0	36.0
32	34.597611410607264	36.0	36.0	36.0	32.0	36.0
33	34.58172486240766	36.0	36.0	36.0	32.0	36.0
34	34.55702670434515	36.0	36.0	36.0	32.0	36.0
35	34.52847850102261	36.0	36.0	36.0	32.0	36.0
36	34.5013578008026	36.0	36.0	36.0	32.0	36.0
37	34.496394377471724	36.0	36.0	36.0	32.0	36.0
38	34.45347386969201	36.0	36.0	36.0	32.0	36.0
39	34.448193223449465	36.0	36.0	36.0	32.0	36.0
40	34.42620694953615	36.0	36.0	36.0	32.0	36.0
41	34.41101680475983	36.0	36.0	36.0	32.0	36.0
42	34.34269925719033	36.0	36.0	36.0	32.0	36.0
43	34.34200099503906	36.0	36.0	36.0	32.0	36.0
44	34.26808929577133	36.0	36.0	36.0	32.0	36.0
45	34.23309750205544	36.0	36.0	36.0	32.0	36.0
46	34.222711549578165	36.0	36.0	36.0	32.0	36.0
47	34.18803202216595	36.0	36.0	36.0	32.0	36.0
48	34.14504212187432	36.0	36.0	36.0	32.0	36.0
49	34.12437802314364	36.0	36.0	36.0	32.0	36.0
50	33.484304590996196	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	4.0
20	13.0
21	55.0
22	152.0
23	504.0
24	1331.0
25	3005.0
26	6161.0
27	11285.0
28	19276.0
29	29699.0
30	43376.0
31	62455.0
32	91171.0
33	148723.0
34	306343.0
35	890451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.88447751853447	17.776173643103444	11.619787035631411	26.71956180273068
2	16.3500315674754	20.251759135515858	36.14454248226304	27.253666814745696
3	18.122603393678194	24.042178412528862	28.783625288955832	29.051592904837108
4	12.436749035165274	15.505694832798122	35.44563313542011	36.6119229966165
5	14.634818808096867	36.57989091744955	32.93858081160738	15.846709462846196
6	35.062905746089534	35.336139608253276	16.168877114154753	13.43207753150244
7	30.725393384291394	30.28097151995004	20.510877586032773	18.482757509725793
8	28.758921119920792	32.49911555526091	18.96038864763288	19.781574677185414
9	27.723247698873354	13.51264442450768	18.069482376616488	40.69462550000248
10	15.840338243120916	26.587264953187358	31.316485200934085	26.255911602757642
11	38.00472984317912	20.605858586734755	22.10163896439111	19.287772605695018
12	24.88873963991482	23.268424486386984	28.56802975451779	23.2748061191804
13	29.684857106390467	19.073877414965818	25.135409089726256	26.10585638891746
14	23.89668935555382	18.997017359291103	24.863042640485848	32.243250644669224
15	25.513494289138023	26.765398013063667	21.86757640609142	25.853531291706886
16	26.218681807536313	25.4872063038492	23.114402867909376	25.179709020705104
17	24.48558153713088	25.59276384798827	24.782730186424224	25.138924428456633
18	25.321715036994906	24.541792285778367	25.696682664128883	24.439810013097844
19	26.077040605797002	24.42568367156008	25.091976114091274	24.40529960855164
20	26.27765582335961	23.852549363658163	24.53402776099743	25.3357670519848
21	27.205272343924157	23.77914098265993	24.094133420734853	24.92145325268106
22	26.222673549755765	23.8982679101167	24.597956386725187	25.281102153402347
23	24.88955968857381	23.65907184289409	25.26453010358155	26.18683836495055
24	25.32702932281866	24.55596316974174	24.81172540999096	25.305282097448632
25	25.394724694898862	23.958021602591863	24.966526701499962	25.680727001009313
26	25.197700766266667	24.671779241837775	25.150424832814828	24.98009515908073
27	25.73040279967086	24.366567032487684	24.380074651280374	25.522955516561087
28	25.009681258879652	24.159837119402606	25.37227151144743	25.45821011027031
29	25.23510408521617	24.3410951655735	25.038941340771736	25.384859408438594
30	24.975076659795324	24.5303290252666	25.306313892198578	25.188280422739506
31	25.491822883039404	24.28096269023864	24.48711005803355	25.740104368688403
32	25.36020322810496	24.37355556243998	24.338796121317266	25.927445088137794
33	24.9238193077006	24.141503389870415	24.87127662606588	26.063400676363102
34	25.644255481562073	24.05840060075987	24.99485735670613	25.30248656097192
35	26.120320355300446	24.04907686569291	24.975061308906426	24.855541470100214
36	25.277203766067597	24.694572413771738	24.50032065481741	25.527903165343258
37	25.92705726505947	24.433021820365056	24.518650194460676	25.121270720114797
38	25.311126959627384	24.266446742685844	24.938549133739514	25.483877163947255
39	25.88449749339969	24.202649542516784	24.378864380871892	25.53398858321163
40	25.955608867817325	24.41698146992728	24.730803713325706	24.89660594892969
41	25.072678201000762	24.740700536071436	25.002354451524322	25.18426681140348
42	26.23737899448319	24.839650651558635	24.40903706126981	24.513933292688368
43	25.566964830615323	23.96274076217372	24.781643493233226	25.688650913977728
44	25.25749881659591	24.428620287139683	24.56771673643175	25.746164159832663
45	25.300622558835776	24.760530463746854	24.484753256537857	25.454093720879513
46	25.254322664348607	24.54260954043791	24.575633227653405	25.62743456756008
47	25.47510000148701	24.386855814460688	24.850369522223378	25.287674661828923
48	26.255115475766356	24.968292569587508	23.92701250019362	24.849579454452517
49	25.379817459614284	24.910176524272938	24.179506926584217	25.530499089528558
50	25.51691962249936	24.967162004084305	24.072357594622996	25.443560778793344
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	25.0
1	27.5
2	30.0
3	101.5
4	173.0
5	218.0
6	263.0
7	264.0
8	265.0
9	343.5
10	422.0
11	646.5
12	871.0
13	1610.5
14	2350.0
15	3087.5
16	3825.0
17	3978.0
18	4131.0
19	3971.0
20	3811.0
21	4295.0
22	4779.0
23	5573.5
24	6368.0
25	7055.5
26	7743.0
27	9435.5
28	11128.0
29	13119.0
30	15110.0
31	17340.0
32	19570.0
33	22942.0
34	26314.0
35	30583.0
36	34852.0
37	42906.5
38	50961.0
39	57361.0
40	63761.0
41	70904.5
42	78048.0
43	81334.5
44	84621.0
45	94376.0
46	104131.0
47	112255.5
48	120380.0
49	125073.0
50	129766.0
51	128150.0
52	126534.0
53	125270.5
54	124007.0
55	125042.0
56	126077.0
57	122598.5
58	119120.0
59	108532.5
60	97945.0
61	86472.5
62	75000.0
63	65007.0
64	55014.0
65	46440.0
66	37866.0
67	32023.0
68	26180.0
69	23386.0
70	20592.0
71	16066.5
72	11541.0
73	9758.0
74	7975.0
75	6168.0
76	4361.0
77	3760.5
78	3160.0
79	2614.5
80	2069.0
81	1671.5
82	1274.0
83	1065.5
84	857.0
85	618.0
86	379.0
87	266.0
88	153.0
89	112.0
90	71.0
91	60.5
92	50.0
93	40.0
94	30.0
95	26.5
96	23.0
97	19.5
98	16.0
99	15.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007558827192199291
2	2.47830399744239E-4
3	3.7174559961635856E-4
4	0.0
5	0.0
6	3.097879996802988E-4
7	0.0
8	0.0
9	9.293639990408964E-4
10	0.0012391519987211951
11	0.0
12	0.0
13	5.576183994245378E-4
14	6.195759993605975E-5
15	0.004027243995843885
16	5.576183994245378E-4
17	0.0
18	6.195759993605975E-5
19	6.195759993605975E-5
20	9.91321598976956E-4
21	0.0
22	1.8587279980817928E-4
23	8.054487991687769E-4
24	4.95660799488478E-4
25	0.002106558397826032
26	0.004832692795012661
27	0.0063816327934141555
28	0.003965286395907824
29	0.00254026159737845
30	0.003593540796291466
31	0.007001208792774753
32	0.0035315831963554064
33	0.00501856559482084
34	0.003159837596739048
35	0.003035922396866928
36	0.007558827192199291
37	0.0034696255964193467
38	0.007187081592582932
39	0.004213116795652064
40	0.002230473597698151
41	0.0026641767972505697
42	0.0019206855980178526
43	0.0011152367988490756
44	0.0011771943987851355
45	0.0014250247985293743
46	8.674063991048367E-4
47	0.0019206855980178526
48	0.001982643197953912
49	4.95660799488478E-4
50	0.0014250247985293743
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1614007.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.35513917205763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.0688255590818	41.120179135318416
2	13.008550368840474	13.881460307124103
3	4.100487624060879	6.5634626356520425
4	1.8347806833228355	3.9157991483557155
5	0.9739968255651252	2.5983868090584794
6	0.6349598781446212	2.032702360024741
7	0.44520634367286377	1.6627832498863997
8	0.3075601148382288	1.312793018477418
9	0.23799089811993157	1.1428233741784743
>10	1.2314769536772787	11.684080171618831
>50	0.08562111072842675	3.146108746390304
>100	0.06024891647071236	6.598077667055799
>500	0.008071999089743345	2.8104850722448473
>1k	0.002222724387030776	1.5308583046145008
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1646	0.10198220949475437	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1631	0.10105284549571346	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.239151998721195E-4
2	0.0	0.0	0.0	0.0	1.239151998721195E-4
3	0.0	0.0	0.0	0.0	1.8587279980817928E-4
4	0.0	0.0	0.0	0.0	1.8587279980817928E-4
5	0.0	0.0	0.0	0.0	2.47830399744239E-4
6	0.0	0.0	0.0	0.0	2.47830399744239E-4
7	0.0	0.0	0.0	0.0	2.47830399744239E-4
8	0.0	0.0	0.0	0.0	2.47830399744239E-4
9	0.0	0.0	0.0	0.0	2.47830399744239E-4
10	0.0	0.0	0.0	6.195759993605975E-5	3.097879996802988E-4
11	0.0	0.0	0.0	6.195759993605975E-5	3.097879996802988E-4
12	0.0	0.0	0.0	6.195759993605975E-5	6.195759993605976E-4
13	0.0	0.0	0.0	6.195759993605975E-5	6.815335992966573E-4
14	0.0	0.0	0.0	6.195759993605975E-5	6.815335992966573E-4
15	0.0	0.0	0.0	6.195759993605975E-5	0.0012391519987211951
16	0.0	0.0	0.0	6.195759993605975E-5	0.0013011095986572548
17	0.0	0.0	0.0	6.195759993605975E-5	0.0013011095986572548
18	0.0	0.0	0.0	6.195759993605975E-5	0.0013011095986572548
19	0.0	0.0	0.0	6.195759993605975E-5	0.0013011095986572548
20	0.0	0.0	0.0	6.195759993605975E-5	0.0013630671985933147
21	0.0	0.0	0.0	6.195759993605975E-5	0.0013630671985933147
22	0.0	0.0	0.0	6.195759993605975E-5	0.001548939998401494
23	0.0	0.0	0.0	1.239151998721195E-4	0.001548939998401494
24	0.0	0.0	0.0	3.7174559961635856E-4	0.0016108975983375536
25	0.0	0.0	0.0	4.337031995524183E-4	0.0016108975983375536
26	0.0	0.0	0.0	4.95660799488478E-4	0.0016728551982736135
27	0.0	0.0	0.0	7.434911992327171E-4	0.0017348127982096731
28	0.0	0.0	0.0	0.0014869823984654342	0.001796770398145733
29	0.0	0.0	0.0	0.004398989595460243	0.001796770398145733
30	0.0	0.0	0.0	0.009479512790217142	0.001796770398145733
31	0.0	0.0	0.0	0.01976447437960306	0.001796770398145733
32	0.0	0.0	0.0	0.03320927356572803	0.001796770398145733
33	0.0	0.0	0.0	0.04485730235370727	0.0019206855980178526
34	0.0	0.0	0.0	0.05774448314040769	0.0019206855980178526
35	0.0	0.0	0.0	0.0742252047233996	0.0019206855980178526
36	0.0	0.0	0.0	0.09919411749763167	0.0019206855980178526
37	0.0	1.239151998721195E-4	0.0	0.13742195665818055	0.0019206855980178526
38	0.0	1.239151998721195E-4	0.0	0.19200660220184917	0.0019206855980178526
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	60	9.761376E-6	25.664717	5
GTATCAA	2615	0.0	22.295525	1
ATACCGT	385	0.0	18.85571	6
CGTTTAT	560	0.0	18.074533	31
GGTATCA	1400	0.0	17.443693	1
AACGCAG	3360	0.0	17.415342	6
TCGTTTA	560	0.0	17.288149	30
TACACCG	115	4.2777738E-6	17.216084	5
TTATACT	205	5.456968E-12	17.169426	4
CATCGTT	570	0.0	16.984324	28
TAATAGT	160	2.1325832E-8	16.498745	4
TACCGTC	400	0.0	16.498745	7
GTCTAGA	215	1.2732926E-11	16.372877	1
GTATAGT	135	1.0854546E-6	16.297077	1
ATCGTTT	595	0.0	16.270695	29
TGGTCGG	615	0.0	16.097834	37
GTATTAG	330	0.0	16.000767	1
GTATAGG	220	1.8189894E-11	16.000767	1
TACTATA	110	5.628266E-5	15.998287	2
TCAACGC	3680	0.0	15.781409	4
>>END_MODULE