FastQCFastQC Report
Thu 2 Feb 2017
SRR4064171_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064171_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3925
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT160.4076433121019108No Hit
TATCAACGCAGAGTACTTTTTTTTT120.3057324840764331No Hit
GGTATCAACGCAGAGTACTTTTTTT80.2038216560509554No Hit
ACGCAGAGTACTTTTTTTTTTTTTT80.2038216560509554No Hit
GAGTACTTTTTTTTTTTTTTTTTTT70.17834394904458598No Hit
CTTTAATATACGCTATTGGAGCTGG70.17834394904458598No Hit
CATCTAAGGGCATCACAGACCTGTT60.15286624203821655No Hit
GAATAATGGAATAGGACCGCGGTTC60.15286624203821655No Hit
ATTCCGGAGAGGGAGCCTGAGAAAC60.15286624203821655No Hit
CTCCAATAGCGTATATTAAAGTTGC60.15286624203821655No Hit
CTCTTAATCATGGCCTCAGTTCCGA50.12738853503184713No Hit
GTACTTTTTTTTTTTTTTTTTTTTT50.12738853503184713No Hit
GCGCAAGACGGACCAGAGCGAAAGC50.12738853503184713No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT50.12738853503184713No Hit
GTATCTGATCGTCTTCGAACCTCCG40.1019108280254777No Hit
GTTCTATTTTGTTGGTTTTCGGAAC40.1019108280254777No Hit
CCTTTAAGTTTCAGCTTTGCAACCA40.1019108280254777No Hit
GTCTTGCGCCGGTCCAAGAATTTCA40.1019108280254777No Hit
GTCCTACAGTGGACATTTCTAAATT40.1019108280254777No Hit
GTCCTAAAGTGTGTATTTCTCATTT40.1019108280254777No Hit
AGTCAGTGTAGCGCGCGTGCAGCCC40.1019108280254777No Hit
GCCTCGAAAGAGTCCTGTATTGTTA40.1019108280254777No Hit
CTTTCGAGGCCCTGTAATTGGAATG40.1019108280254777No Hit
ATACAGGACTCTTTCGAGGCCCTGT40.1019108280254777No Hit
ACCATACTCCCCCCGGAACCCAAAG40.1019108280254777No Hit
GTATTGTTATTTTTCGTCACTACCT40.1019108280254777No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)