##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064171_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3925
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.44738853503185	32.0	32.0	32.0	14.0	32.0
2	27.985732484076433	32.0	32.0	32.0	14.0	32.0
3	28.220382165605095	32.0	27.0	32.0	14.0	32.0
4	27.897579617834396	32.0	21.0	32.0	14.0	32.0
5	28.051974522292994	32.0	21.0	32.0	14.0	32.0
6	31.369426751592357	36.0	32.0	36.0	21.0	36.0
7	31.40433121019108	36.0	32.0	36.0	14.0	36.0
8	31.211210191082802	36.0	32.0	36.0	14.0	36.0
9	31.392101910828025	36.0	32.0	36.0	14.0	36.0
10	30.31872611464968	36.0	21.0	36.0	14.0	36.0
11	31.765859872611465	36.0	32.0	36.0	14.0	36.0
12	30.87847133757962	36.0	32.0	36.0	14.0	36.0
13	31.42777070063694	36.0	32.0	36.0	14.0	36.0
14	30.911847133757963	36.0	32.0	36.0	14.0	36.0
15	30.63923566878981	36.0	27.0	36.0	14.0	36.0
16	30.926624203821657	36.0	32.0	36.0	14.0	36.0
17	30.772738853503185	36.0	27.0	36.0	14.0	36.0
18	30.56076433121019	36.0	27.0	36.0	14.0	36.0
19	30.793885350318472	36.0	32.0	36.0	14.0	36.0
20	30.6856050955414	36.0	27.0	36.0	14.0	36.0
21	30.50496815286624	36.0	27.0	36.0	14.0	36.0
22	30.513630573248406	36.0	27.0	36.0	14.0	36.0
23	30.407388535031846	36.0	27.0	36.0	14.0	36.0
24	30.432611464968154	36.0	27.0	36.0	14.0	36.0
25	29.533248407643313	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	10.0
16	19.0
17	28.0
18	50.0
19	61.0
20	105.0
21	104.0
22	122.0
23	83.0
24	107.0
25	130.0
26	141.0
27	145.0
28	156.0
29	204.0
30	204.0
31	247.0
32	290.0
33	377.0
34	607.0
35	732.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7624872579001	18.47604485219164	10.958205912334353	27.803261977573907
2	15.51592356687898	19.97452229299363	40.48407643312102	24.02547770700637
3	17.808917197452228	22.828025477707005	28.662420382165603	30.700636942675157
4	11.312101910828025	15.770700636942674	36.68789808917197	36.22929936305733
5	13.528662420382165	35.03184713375796	36.025477707006374	15.414012738853502
6	31.566878980891723	37.52866242038216	16.50955414012739	14.394904458598726
7	30.1656050955414	29.426751592356688	21.987261146496813	18.420382165605094
8	26.191082802547772	33.910828025477706	20.968152866242036	18.929936305732483
9	26.929936305732483	13.630573248407643	18.038216560509554	41.40127388535032
10	15.341488277268095	27.803261977573907	31.549439347604487	25.30581039755352
11	36.38216560509554	21.528662420382165	23.286624203821656	18.802547770700638
12	25.178389398572886	23.21610601427115	29.357798165137616	22.24770642201835
13	28.822629969418962	20.438328236493376	25.127420998980632	25.611620795107033
14	22.50254841997961	19.77573904179409	26.019367991845055	31.702344546381244
15	23.388535031847134	28.9171974522293	21.477707006369425	26.21656050955414
16	25.044585987261147	26.038216560509554	24.89171974522293	24.02547770700637
17	23.031847133757964	25.70700636942675	27.133757961783438	24.127388535031848
18	22.884811416921508	25.637104994903158	28.899082568807337	22.579001019367993
19	24.79612640163099	24.84709480122324	26.758409785932724	23.598369011213048
20	23.267074413863405	25.101936799184504	26.580020387359838	25.050968399592254
21	25.286770328829977	25.286770328829977	24.598521539638032	24.827937802702014
22	23.700305810397555	25.840978593272173	25.840978593272173	24.617737003058103
23	22.431812388478207	25.363242416517974	27.5044608717818	24.700484323222025
24	24.49655875605404	24.13968901351007	27.249553912821824	24.114198317614072
25	24.0	24.229299363057326	26.85350318471337	24.9171974522293
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	2.0
5	2.0
6	4.0
7	6.0
8	6.0
9	6.0
10	9.5
11	13.0
12	13.0
13	13.0
14	16.5
15	20.0
16	20.0
17	20.0
18	20.5
19	21.0
20	21.0
21	21.0
22	45.5
23	70.0
24	70.0
25	70.0
26	101.0
27	132.0
28	132.0
29	132.0
30	179.5
31	227.0
32	227.0
33	227.0
34	262.5
35	298.0
36	298.0
37	298.0
38	325.5
39	353.0
40	353.0
41	353.0
42	392.0
43	431.0
44	431.0
45	431.0
46	458.0
47	485.0
48	485.0
49	485.0
50	498.0
51	511.0
52	511.0
53	511.0
54	468.5
55	426.0
56	426.0
57	426.0
58	373.5
59	321.0
60	321.0
61	321.0
62	290.0
63	259.0
64	259.0
65	259.0
66	204.5
67	150.0
68	150.0
69	150.0
70	117.0
71	84.0
72	84.0
73	84.0
74	66.0
75	48.0
76	48.0
77	48.0
78	45.0
79	42.0
80	42.0
81	42.0
82	28.0
83	14.0
84	14.0
85	14.0
86	11.0
87	8.0
88	8.0
89	8.0
90	5.0
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025477707006369425
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.025477707006369425
11	0.0
12	0.025477707006369425
13	0.025477707006369425
14	0.025477707006369425
15	0.0
16	0.0
17	0.0
18	0.025477707006369425
19	0.025477707006369425
20	0.025477707006369425
21	0.05095541401273885
22	0.025477707006369425
23	0.05095541401273885
24	0.05095541401273885
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3925.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.12738853503186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.07743362831859	87.59235668789809
2	3.2909292035398234	6.063694267515923
3	0.9126106194690266	2.522292993630573
4	0.33185840707964603	1.2229299363057324
5	0.11061946902654868	0.5095541401273885
6	0.11061946902654868	0.6114649681528662
7	0.05530973451327434	0.35668789808917195
8	0.05530973451327434	0.4076433121019108
9	0.0	0.0
>10	0.05530973451327434	0.7133757961783439
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	16	0.4076433121019108	No Hit
TATCAACGCAGAGTACTTTTTTTTT	12	0.3057324840764331	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8	0.2038216560509554	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	8	0.2038216560509554	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	7	0.17834394904458598	No Hit
CTTTAATATACGCTATTGGAGCTGG	7	0.17834394904458598	No Hit
CATCTAAGGGCATCACAGACCTGTT	6	0.15286624203821655	No Hit
GAATAATGGAATAGGACCGCGGTTC	6	0.15286624203821655	No Hit
ATTCCGGAGAGGGAGCCTGAGAAAC	6	0.15286624203821655	No Hit
CTCCAATAGCGTATATTAAAGTTGC	6	0.15286624203821655	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	5	0.12738853503184713	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5	0.12738853503184713	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	5	0.12738853503184713	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	5	0.12738853503184713	No Hit
GTATCTGATCGTCTTCGAACCTCCG	4	0.1019108280254777	No Hit
GTTCTATTTTGTTGGTTTTCGGAAC	4	0.1019108280254777	No Hit
CCTTTAAGTTTCAGCTTTGCAACCA	4	0.1019108280254777	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	4	0.1019108280254777	No Hit
GTCCTACAGTGGACATTTCTAAATT	4	0.1019108280254777	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4	0.1019108280254777	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCC	4	0.1019108280254777	No Hit
GCCTCGAAAGAGTCCTGTATTGTTA	4	0.1019108280254777	No Hit
CTTTCGAGGCCCTGTAATTGGAATG	4	0.1019108280254777	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	4	0.1019108280254777	No Hit
ACCATACTCCCCCCGGAACCCAAAG	4	0.1019108280254777	No Hit
GTATTGTTATTTTTCGTCACTACCT	4	0.1019108280254777	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE