##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064171_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3925
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.18140127388535	32.0	32.0	32.0	21.0	32.0
2	29.85375796178344	32.0	32.0	32.0	21.0	32.0
3	30.788789808917198	32.0	32.0	32.0	32.0	32.0
4	31.108025477707006	32.0	32.0	32.0	32.0	32.0
5	30.916687898089172	32.0	32.0	32.0	32.0	32.0
6	34.45707006369427	36.0	36.0	36.0	32.0	36.0
7	34.476687898089175	36.0	36.0	36.0	32.0	36.0
8	34.334777070063694	36.0	36.0	36.0	32.0	36.0
9	34.53171974522293	36.0	36.0	36.0	32.0	36.0
10	34.40382165605096	36.0	36.0	36.0	32.0	36.0
11	34.80101910828026	36.0	36.0	36.0	32.0	36.0
12	34.641783439490446	36.0	36.0	36.0	32.0	36.0
13	34.580382165605094	36.0	36.0	36.0	32.0	36.0
14	34.600509554140125	36.0	36.0	36.0	32.0	36.0
15	34.35796178343949	36.0	36.0	36.0	32.0	36.0
16	34.567898089171976	36.0	36.0	36.0	32.0	36.0
17	34.50343949044586	36.0	36.0	36.0	32.0	36.0
18	34.530191082802546	36.0	36.0	36.0	32.0	36.0
19	34.4456050955414	36.0	36.0	36.0	32.0	36.0
20	34.580891719745225	36.0	36.0	36.0	32.0	36.0
21	34.42496815286624	36.0	36.0	36.0	32.0	36.0
22	34.54471337579618	36.0	36.0	36.0	32.0	36.0
23	34.33757961783439	36.0	36.0	36.0	32.0	36.0
24	34.33452229299363	36.0	36.0	36.0	32.0	36.0
25	34.34292993630573	36.0	36.0	36.0	32.0	36.0
26	34.183184713375795	36.0	36.0	36.0	32.0	36.0
27	34.13707006369427	36.0	36.0	36.0	32.0	36.0
28	34.20127388535032	36.0	36.0	36.0	32.0	36.0
29	34.223694267515924	36.0	36.0	36.0	32.0	36.0
30	34.12535031847134	36.0	36.0	36.0	32.0	36.0
31	34.11057324840764	36.0	36.0	36.0	32.0	36.0
32	34.08382165605096	36.0	36.0	36.0	32.0	36.0
33	34.16764331210191	36.0	36.0	36.0	32.0	36.0
34	34.13171974522293	36.0	36.0	36.0	32.0	36.0
35	34.092738853503185	36.0	36.0	36.0	32.0	36.0
36	34.048917197452226	36.0	36.0	36.0	32.0	36.0
37	33.89885350318471	36.0	36.0	36.0	32.0	36.0
38	33.97910828025478	36.0	36.0	36.0	32.0	36.0
39	33.759490445859875	36.0	36.0	36.0	32.0	36.0
40	33.78700636942675	36.0	36.0	36.0	32.0	36.0
41	33.72127388535032	36.0	36.0	36.0	27.0	36.0
42	33.63388535031847	36.0	36.0	36.0	27.0	36.0
43	33.64713375796178	36.0	36.0	36.0	27.0	36.0
44	33.718726114649684	36.0	36.0	36.0	32.0	36.0
45	33.53095541401274	36.0	36.0	36.0	21.0	36.0
46	33.43821656050955	36.0	36.0	36.0	21.0	36.0
47	33.47515923566879	36.0	36.0	36.0	21.0	36.0
48	33.365095541401274	36.0	36.0	36.0	21.0	36.0
49	33.443057324840765	36.0	36.0	36.0	21.0	36.0
50	32.73324840764331	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	1.0
23	2.0
24	2.0
25	9.0
26	19.0
27	32.0
28	77.0
29	111.0
30	169.0
31	251.0
32	352.0
33	577.0
34	904.0
35	1419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.81957186544342	18.527013251783895	12.181447502548421	27.47196738022426
2	17.579617834394902	21.35031847133758	36.203821656050955	24.86624203821656
3	20.53503184713376	23.312101910828027	30.14012738853503	26.012738853503187
4	14.445859872611466	16.280254777070063	36.738853503184714	32.53503184713376
5	18.980891719745223	33.35031847133758	31.796178343949045	15.872611464968154
6	35.00636942675159	36.66242038216561	14.21656050955414	14.114649681528663
7	29.273885350318473	30.675159235668794	19.159235668789808	20.891719745222932
8	27.133757961783438	34.62420382165605	18.24203821656051	20.0
9	26.08917197452229	13.121019108280255	18.19108280254777	42.59872611464968
10	14.675159235668788	27.18471337579618	31.46496815286624	26.675159235668787
11	38.21656050955414	19.796178343949045	23.337579617834393	18.64968152866242
12	24.86624203821656	23.56687898089172	29.17197452229299	22.394904458598727
13	30.828025477707005	18.777070063694268	24.611464968152866	25.783439490445858
14	23.159235668789808	18.980891719745223	24.713375796178344	33.146496815286625
15	24.15286624203822	28.254777070063696	21.579617834394902	26.012738853503187
16	25.88535031847134	26.62420382165605	23.745222929936304	23.745222929936304
17	25.248407643312103	24.53503184713376	26.063694267515924	24.15286624203822
18	24.611464968152866	27.05732484076433	25.324840764331206	23.00636942675159
19	26.929936305732483	24.280254777070066	25.197452229299362	23.59235668789809
20	25.27388535031847	24.331210191082803	25.401273885350317	24.993630573248407
21	26.063694267515924	24.53503184713376	23.796178343949045	25.605095541401273
22	25.452229299363054	23.51592356687898	25.248407643312103	25.783439490445858
23	23.770700636942678	24.331210191082803	26.802547770700635	25.095541401273884
24	25.044585987261147	24.305732484076433	24.662420382165607	25.987261146496817
25	23.414012738853504	24.484076433121018	26.522292993630575	25.579617834394902
26	24.738853503184714	24.86624203821656	25.528662420382165	24.86624203821656
27	25.095541401273884	25.65605095541401	25.75796178343949	23.49044585987261
28	23.872611464968152	25.171974522292995	27.133757961783438	23.821656050955415
29	25.452229299363054	25.197452229299362	25.324840764331206	24.02547770700637
30	25.171974522292995	23.97452229299363	25.834394904458595	25.019108280254777
31	25.961783439490443	24.662420382165607	25.171974522292995	24.203821656050955
32	24.687898089171973	24.636942675159233	25.35031847133758	25.324840764331206
33	23.745222929936304	25.452229299363054	25.605095541401273	25.197452229299362
34	24.076433121019107	25.783439490445858	25.452229299363054	24.687898089171973
35	25.88535031847134	24.993630573248407	23.923566878980893	25.197452229299362
36	23.617834394904456	25.85987261146497	25.197452229299362	25.324840764331206
37	24.81528662420382	24.43312101910828	25.070063694267514	25.681528662420384
38	24.56050955414013	25.681528662420384	24.94267515923567	24.81528662420382
39	25.75796178343949	24.50955414012739	24.305732484076433	25.426751592356688
40	25.681528662420384	25.019108280254777	25.044585987261147	24.254777070063692
41	26.496815286624205	24.94267515923567	25.121019108280258	23.439490445859875
42	26.11464968152866	26.369426751592357	23.46496815286624	24.05095541401274
43	23.898089171974522	26.62420382165605	25.426751592356688	24.05095541401274
44	25.171974522292995	25.070063694267514	25.783439490445858	23.97452229299363
45	24.02547770700637	26.343949044585983	24.713375796178344	24.9171974522293
46	22.980891719745223	26.24203821656051	25.37579617834395	25.401273885350317
47	25.528662420382165	24.178343949044585	25.528662420382165	24.76433121019108
48	24.86624203821656	25.248407643312103	24.89171974522293	24.993630573248407
49	23.949044585987263	24.687898089171973	25.146496815286625	26.21656050955414
50	24.96815286624204	25.299363057324843	24.76433121019108	24.96815286624204
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	1.0
6	0.0
7	0.5
8	1.0
9	2.0
10	3.0
11	3.0
12	3.0
13	3.5
14	4.0
15	4.5
16	5.0
17	6.5
18	8.0
19	10.5
20	13.0
21	12.0
22	11.0
23	17.0
24	23.0
25	26.5
26	30.0
27	39.5
28	49.0
29	51.5
30	54.0
31	64.5
32	75.0
33	91.5
34	108.0
35	125.0
36	142.0
37	153.5
38	165.0
39	163.5
40	162.0
41	189.5
42	217.0
43	206.5
44	196.0
45	217.5
46	239.0
47	242.5
48	246.0
49	255.5
50	265.0
51	262.5
52	260.0
53	272.0
54	284.0
55	274.5
56	265.0
57	269.0
58	273.0
59	230.0
60	187.0
61	174.5
62	162.0
63	140.0
64	118.0
65	110.5
66	103.0
67	89.5
68	76.0
69	66.5
70	57.0
71	44.5
72	32.0
73	27.0
74	22.0
75	19.5
76	17.0
77	18.0
78	19.0
79	13.0
80	7.0
81	6.5
82	6.0
83	6.0
84	6.0
85	4.0
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	3.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025477707006369425
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3925.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.49044585987261
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.58484525080043	92.22929936305732
2	2.5346851654215583	4.840764331210192
3	0.5336179295624333	1.5286624203821657
4	0.26680896478121663	1.019108280254777
5	0.08004268943436499	0.3821656050955414
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	5	0.12738853503184713	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	5	0.12738853503184713	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	5	0.12738853503184713	No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG	4	0.1019108280254777	No Hit
GGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG	4	0.1019108280254777	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	4	0.1019108280254777	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	4	0.1019108280254777	No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC	4	0.1019108280254777	No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA	4	0.1019108280254777	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	4	0.1019108280254777	No Hit
AAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTT	4	0.1019108280254777	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	4	0.1019108280254777	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	4	0.1019108280254777	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.025477707006369428	0.0
33	0.0	0.0	0.0	0.025477707006369428	0.0
34	0.0	0.0	0.0	0.050955414012738856	0.0
35	0.0	0.0	0.0	0.050955414012738856	0.0
36	0.0	0.0	0.0	0.07643312101910828	0.0
37	0.0	0.0	0.0	0.12738853503184713	0.0
38	0.0	0.0	0.0	0.22929936305732485	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE