FastQCFastQC Report
Thu 2 Feb 2017
SRR4064170_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064170_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772146
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26190.33918455836072453No Hit
TATCAACGCAGAGTACTTTTTTTTT19070.2469740178670873No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16070.20812126204111658No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10220.13235838818047363No Hit
GGTATCAACGCAGAGTACTTTTTTT9930.12860262178396314No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9160.11863041445529732No Hit
GTACATGGGGTGGTATCAACGCAAA8110.10503194991620754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG551.1280788E-515.5436521
CGTTATT704.4562148E-714.9200692
ACGAACG801.2802411E-714.25481615
CCGACTT400.00527089714.25204312
CGACTTT400.00527089714.25204313
GGATAAG400.005279974514.2483471
ATTCCGA801.2883902E-714.2474236
TCTAGAT1101.8735591E-1013.8103122
TTCCGAT903.6317942E-813.721527
CGAACGA852.684119E-713.41542716
CCGATAA852.6904854E-713.4128189
CGATAAC852.6904854E-713.41281810
GCGTTAT852.69687E-713.4102091
GCGCCGA500.001494542713.30449419
CAGCGCA500.001497467413.30104359
ACTTAAC500.00149966413.2984571
CGACCAT655.4415344E-513.15487910
TCAATAC655.4747332E-513.1463553
TTGCGCG751.47117735E-512.67094718
ACGCCCC604.078467E-412.67012517
Produced by FastQC (version 0.11.5)