##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064170_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 772146 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.182595001463454 32.0 32.0 32.0 32.0 32.0 2 30.73327842143843 32.0 32.0 32.0 32.0 32.0 3 30.75036068308325 32.0 32.0 32.0 32.0 32.0 4 30.78389838191223 32.0 32.0 32.0 32.0 32.0 5 30.679905095668435 32.0 32.0 32.0 32.0 32.0 6 34.325797193794955 36.0 36.0 36.0 32.0 36.0 7 34.255709412468626 36.0 36.0 36.0 32.0 36.0 8 34.19496183364286 36.0 36.0 36.0 32.0 36.0 9 34.36496336185126 36.0 36.0 36.0 32.0 36.0 10 34.01887207859654 36.0 36.0 36.0 32.0 36.0 11 34.35045962810142 36.0 36.0 36.0 32.0 36.0 12 34.1349718835557 36.0 36.0 36.0 32.0 36.0 13 34.23952853475897 36.0 36.0 36.0 32.0 36.0 14 34.13016061729258 36.0 36.0 36.0 32.0 36.0 15 34.05957293050796 36.0 36.0 36.0 32.0 36.0 16 34.046623306991165 36.0 36.0 36.0 32.0 36.0 17 33.96836867639021 36.0 36.0 36.0 32.0 36.0 18 33.97088892515146 36.0 36.0 36.0 32.0 36.0 19 33.988349353619654 36.0 36.0 36.0 32.0 36.0 20 33.95195338705374 36.0 36.0 36.0 32.0 36.0 21 33.94830640837355 36.0 36.0 36.0 32.0 36.0 22 33.921727238113 36.0 36.0 36.0 32.0 36.0 23 33.85891398776915 36.0 36.0 36.0 32.0 36.0 24 33.85923517055065 36.0 36.0 36.0 32.0 36.0 25 33.44877911690276 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 6.0 5 12.0 6 28.0 7 16.0 8 39.0 9 45.0 10 82.0 11 21.0 12 35.0 13 48.0 14 86.0 15 143.0 16 260.0 17 343.0 18 378.0 19 564.0 20 856.0 21 1304.0 22 1943.0 23 3030.0 24 4599.0 25 6660.0 26 9659.0 27 12626.0 28 17020.0 29 22825.0 30 29894.0 31 40632.0 32 56932.0 33 79510.0 34 164060.0 35 318483.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.5111170997721 17.377165149275292 11.448261208117342 25.663456542835267 2 16.094200477141502 20.142520768089025 38.743397858821034 25.019880895948443 3 19.071196283541823 24.422994484110898 28.79141287835127 27.71439635399601 4 12.294121227741392 15.762507495625663 36.5557292482875 35.38764202834545 5 13.964943547791714 37.24511669293807 34.055375879616975 14.734563879653242 6 33.117455054142276 36.207838972073986 17.31205637013626 13.36264960364748 7 29.15021503704855 30.995258821700606 21.215088864708015 18.639437276542825 8 27.305081232347835 33.98466043064804 19.823284015235924 18.886974321768196 9 27.387449724260893 14.255633992619316 18.740281751461623 39.61663453165817 10 15.527384497365604 27.372316687270153 32.42030078500584 24.679998030358398 11 36.67495982999015 21.240475820245685 23.253900378375576 18.830663971388585 12 24.580206290847027 23.86729892215041 29.88716079877765 21.665333988224912 13 29.71059851456727 19.981675083945998 25.51478888115631 24.79293752033042 14 22.971438791300223 20.314901646695336 25.957418682591893 30.756240879412548 15 24.46046148782264 28.46938749063729 22.81728907653192 24.25286194500815 16 24.471795164218797 26.15759250307469 25.382602644037313 23.988009688669205 17 23.07791995728567 26.427677226886182 26.20386761668036 24.290535199147786 18 23.616603480120542 25.51647710702829 27.70772171997019 23.15919769288098 19 25.01678448206588 25.044780220492363 26.082825910574353 23.855609386867407 20 25.322742873733656 25.04134738020925 25.85351880463298 23.78239094142411 21 26.05146031474468 24.53722785161222 25.193701995733242 24.21760983790986 22 25.100159815013797 25.334892166990485 25.73190221343146 23.833045804564254 23 23.96115528294892 24.979195885322017 26.194000637733613 24.86564819399545 24 24.28056131964813 25.349463863783946 26.357189980026934 24.01278483654099 25 24.284277433871488 25.253393266349374 26.4774577202642 23.984871579514934 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 399.0 1 399.0 2 478.0 3 557.0 4 557.0 5 557.0 6 1371.0 7 2185.0 8 2185.0 9 2185.0 10 2164.5 11 2144.0 12 2144.0 13 2144.0 14 2283.5 15 2423.0 16 2423.0 17 2423.0 18 4106.0 19 5789.0 20 5789.0 21 5789.0 22 9511.5 23 13234.0 24 13234.0 25 13234.0 26 20053.5 27 26873.0 28 26873.0 29 26873.0 30 34575.0 31 42277.0 32 42277.0 33 42277.0 34 48475.5 35 54674.0 36 54674.0 37 54674.0 38 60972.5 39 67271.0 40 67271.0 41 67271.0 42 74977.0 43 82683.0 44 82683.0 45 82683.0 46 90988.0 47 99293.0 48 99293.0 49 99293.0 50 100656.0 51 102019.0 52 102019.0 53 102019.0 54 93223.5 55 84428.0 56 84428.0 57 84428.0 58 76761.5 59 69095.0 60 69095.0 61 69095.0 62 59986.5 63 50878.0 64 50878.0 65 50878.0 66 41517.0 67 32156.0 68 32156.0 69 32156.0 70 24303.5 71 16451.0 72 16451.0 73 16451.0 74 12475.5 75 8500.0 76 8500.0 77 8500.0 78 6687.0 79 4874.0 80 4874.0 81 4874.0 82 3369.5 83 1865.0 84 1865.0 85 1865.0 86 1425.0 87 985.0 88 985.0 89 985.0 90 728.5 91 472.0 92 472.0 93 472.0 94 330.0 95 188.0 96 188.0 97 188.0 98 310.5 99 433.0 100 433.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.041054411989442416 2 0.006216440932155318 3 0.0016836194191253986 4 0.0037557663965105043 5 0.009972207328665822 6 0.013727973725176328 7 0.024088708612101856 8 0.03703962722075877 9 0.052062692806800794 10 0.05594796838939786 11 0.05517091327287845 12 0.06695624920675623 13 0.06721526757892937 14 0.06993496048674733 15 0.06061029908851434 16 0.06773330432327565 17 0.06579066653197711 18 0.08042520455975942 19 0.07770551165194146 20 0.08210882397888483 21 0.07770551165194146 22 0.08146127804845198 23 0.08599409956148191 24 0.07861207595454746 25 0.08003667700149972 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 772146.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.186932768375904 #Duplication Level Percentage of deduplicated Percentage of total 1 81.75975215954912 50.84388210704959 2 11.823628285524478 14.70550354540357 3 3.0838513008236847 5.753257605359732 4 1.1481073920051628 2.8558910879000186 5 0.5756623220027123 1.7899337057834914 6 0.36751178920688543 1.3712658556196475 7 0.21976140510209718 0.9566401406917557 8 0.1562430561582846 0.777302114307266 9 0.11643459782287448 0.6516639456051584 >10 0.6089240861401211 7.111180247983557 >50 0.07215373677506182 3.213345015479449 >100 0.06357796967167408 7.66917426089144 >500 0.0035553469859105295 1.3704057990520544 >1k 8.365522319789482E-4 0.9305545688732514 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2619 0.33918455836072453 No Hit TATCAACGCAGAGTACTTTTTTTTT 1907 0.2469740178670873 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1607 0.20812126204111658 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1022 0.13235838818047363 No Hit GGTATCAACGCAGAGTACTTTTTTT 993 0.12860262178396314 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 916 0.11863041445529732 No Hit GTACATGGGGTGGTATCAACGCAAA 811 0.10503194991620754 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.2950918608656912E-4 0.0 5 0.0 0.0 0.0 1.2950918608656912E-4 0.0 6 0.0 0.0 0.0 1.2950918608656912E-4 0.0 7 0.0 0.0 0.0 2.5901837217313825E-4 0.0 8 0.0 0.0 0.0 2.5901837217313825E-4 0.0 9 0.0 0.0 0.0 2.5901837217313825E-4 0.0 10 0.0 0.0 0.0 2.5901837217313825E-4 0.0 11 0.0 0.0 0.0 2.5901837217313825E-4 0.0 12 0.0 0.0 0.0 2.5901837217313825E-4 0.0 13 0.0 0.0 0.0 2.5901837217313825E-4 2.5901837217313825E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 55 1.1280788E-5 15.543652 1 CGTTATT 70 4.4562148E-7 14.920069 2 ACGAACG 80 1.2802411E-7 14.254816 15 CCGACTT 40 0.005270897 14.252043 12 CGACTTT 40 0.005270897 14.252043 13 GGATAAG 40 0.0052799745 14.248347 1 ATTCCGA 80 1.2883902E-7 14.247423 6 TCTAGAT 110 1.8735591E-10 13.810312 2 TTCCGAT 90 3.6317942E-8 13.72152 7 CGAACGA 85 2.684119E-7 13.415427 16 CCGATAA 85 2.6904854E-7 13.412818 9 CGATAAC 85 2.6904854E-7 13.412818 10 GCGTTAT 85 2.69687E-7 13.410209 1 GCGCCGA 50 0.0014945427 13.304494 19 CAGCGCA 50 0.0014974674 13.3010435 9 ACTTAAC 50 0.001499664 13.298457 1 CGACCAT 65 5.4415344E-5 13.154879 10 TCAATAC 65 5.4747332E-5 13.146355 3 TTGCGCG 75 1.47117735E-5 12.670947 18 ACGCCCC 60 4.078467E-4 12.670125 17 >>END_MODULE