FastQCFastQC Report
Thu 2 Feb 2017
SRR4064170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772146
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.11565170317530624No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGAGT602.8715623E-421.9999034
ATTATAC602.8728205E-421.9984783
GTTATAC904.0805753E-721.9984783
ACATTAG755.469538E-520.5332431
GTATAGT755.469538E-520.5332431
GTATCAA20800.019.3556841
TATATCA4300.018.4185244
TACGTCT600.00740992818.3332524
TTAACGG600.007412696318.33206635
ATAACCC600.007412696318.3320663
CGGTCCG851.4307429E-418.11639439
ATTGCAC851.4307429E-418.1163943
TAATACT1601.0986696E-917.874924
TCTATAC750.001290895917.5987833
GATATAC3650.017.4793761
GGTCCGC1307.313665E-716.92190740
TTATACT1053.7716392E-516.7618314
GTGTATA4750.016.673611
GAGTAGT800.001986672916.50099613
TTATACC800.001987533216.4999264
Produced by FastQC (version 0.11.5)