##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064170_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	772146
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266536380425464	32.0	32.0	32.0	32.0	32.0
2	31.388878528154002	32.0	32.0	32.0	32.0	32.0
3	31.465369243640453	32.0	32.0	32.0	32.0	32.0
4	31.54740554247513	32.0	32.0	32.0	32.0	32.0
5	31.495147290797338	32.0	32.0	32.0	32.0	32.0
6	35.0681231269734	36.0	36.0	36.0	36.0	36.0
7	35.08675302339195	36.0	36.0	36.0	36.0	36.0
8	35.028221346740125	36.0	36.0	36.0	36.0	36.0
9	35.140197579214295	36.0	36.0	36.0	36.0	36.0
10	35.00009195152212	36.0	36.0	36.0	36.0	36.0
11	35.13403423704843	36.0	36.0	36.0	36.0	36.0
12	35.04757649460076	36.0	36.0	36.0	36.0	36.0
13	35.09041942845006	36.0	36.0	36.0	36.0	36.0
14	35.03359209268714	36.0	36.0	36.0	36.0	36.0
15	35.00900995407604	36.0	36.0	36.0	36.0	36.0
16	35.01491427786973	36.0	36.0	36.0	36.0	36.0
17	34.996299922553504	36.0	36.0	36.0	36.0	36.0
18	34.96785841019704	36.0	36.0	36.0	36.0	36.0
19	34.976077063146086	36.0	36.0	36.0	36.0	36.0
20	34.95851561751275	36.0	36.0	36.0	36.0	36.0
21	34.958984440766386	36.0	36.0	36.0	36.0	36.0
22	34.93784983668892	36.0	36.0	36.0	36.0	36.0
23	34.89527628194668	36.0	36.0	36.0	32.0	36.0
24	34.873860642935405	36.0	36.0	36.0	32.0	36.0
25	34.83569428579569	36.0	36.0	36.0	32.0	36.0
26	34.77637519329246	36.0	36.0	36.0	32.0	36.0
27	34.7764036853134	36.0	36.0	36.0	32.0	36.0
28	34.74777179445338	36.0	36.0	36.0	32.0	36.0
29	34.71367332084865	36.0	36.0	36.0	32.0	36.0
30	34.688715605597906	36.0	36.0	36.0	32.0	36.0
31	34.68070675753031	36.0	36.0	36.0	32.0	36.0
32	34.639421819189636	36.0	36.0	36.0	32.0	36.0
33	34.61277012378488	36.0	36.0	36.0	32.0	36.0
34	34.59050878978846	36.0	36.0	36.0	32.0	36.0
35	34.55066269850521	36.0	36.0	36.0	32.0	36.0
36	34.514626767476614	36.0	36.0	36.0	32.0	36.0
37	34.49325775177233	36.0	36.0	36.0	32.0	36.0
38	34.44779743727223	36.0	36.0	36.0	32.0	36.0
39	34.43112183447172	36.0	36.0	36.0	32.0	36.0
40	34.412784628813725	36.0	36.0	36.0	32.0	36.0
41	34.380487627987456	36.0	36.0	36.0	32.0	36.0
42	34.32150784955177	36.0	36.0	36.0	32.0	36.0
43	34.30812178007786	36.0	36.0	36.0	32.0	36.0
44	34.23377314652928	36.0	36.0	36.0	32.0	36.0
45	34.195886529231515	36.0	36.0	36.0	32.0	36.0
46	34.160512390143836	36.0	36.0	36.0	32.0	36.0
47	34.09412209608028	36.0	36.0	36.0	32.0	36.0
48	34.0503518764586	36.0	36.0	36.0	32.0	36.0
49	34.019581788936286	36.0	36.0	36.0	32.0	36.0
50	33.43943373403476	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	29.0
22	90.0
23	261.0
24	683.0
25	1573.0
26	3182.0
27	6007.0
28	9644.0
29	14747.0
30	20952.0
31	29382.0
32	42377.0
33	65746.0
34	132957.0
35	444504.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.551378627899986	18.17460029634542	12.022971950802516	26.251049124952075
2	15.394537806419528	20.925889471393937	38.25633560579374	25.423237116392798
3	18.339690084116324	24.981965822481527	29.159937576491462	27.51840651691068
4	12.032439460930963	16.477583254980278	36.62610438958435	34.863872894504404
5	13.554042888262064	37.678884563282075	33.850981550121354	14.91609099833451
6	33.29197662612155	36.494229055720176	16.99139020700802	13.22240411115025
7	29.1603261045529	31.03458547293578	21.239275006637353	18.56581341587396
8	27.29108225646445	34.247927205476685	19.67127978387507	18.789710754183794
9	27.548961455076682	14.00462093563586	18.649904550740928	39.79651305854653
10	15.46239143779998	27.419968969277512	32.60362784890784	24.51401174401467
11	36.853263501980194	21.12540892525507	23.22786105218443	18.793466520580303
12	24.564039137726724	23.96324524538785	29.824709089614	21.64800652727143
13	29.87441380373223	19.988628990376085	25.49294880843993	24.644008397451756
14	22.950471481245003	20.244100494598786	26.01662131657301	30.7888067075832
15	24.51499849114959	28.43580780483635	22.782346061641395	24.266847642372664
16	24.59087730204367	26.10511047219416	25.320796746703966	23.983215479058202
17	23.1018486141222	26.489808922146846	26.15606374960176	24.252278714129194
18	23.75401965698064	25.406822311411226	27.725822807433335	23.113335224174797
19	25.055786154154987	24.90969960305383	26.34971410810146	23.68480013468973
20	25.43311277833251	24.890855709369898	25.991170029385746	23.684861482911845
21	25.95480647442323	24.518808619095353	25.279675087353947	24.24670981912747
22	25.132747259578526	25.203847986903998	25.963162311693154	23.700242441824322
23	23.97960967763242	24.98280732155554	26.238811807840897	24.79877119297114
24	24.49164340894521	25.179597096400286	26.50344171107758	23.825317783576917
25	24.397417550039822	25.072429044143835	26.523859169704778	24.00629423611156
26	23.592250236691832	26.014860750083212	26.907490082256082	23.485398930968877
27	24.166750852871008	25.49171219197563	26.296402788247086	24.045134166906273
28	23.6699809483845	25.664507656262746	26.390308154376367	24.27520324097639
29	23.75181642279329	25.159236493714726	26.298175676900797	24.790771406591187
30	23.74840535412471	25.72712614053606	26.436346917233834	24.0881215881054
31	24.52716065852341	25.22358115817954	25.871432253451985	24.377825929845056
32	24.081883220829813	25.877579248928928	25.74262683195963	24.297910698281626
33	23.700089753934346	25.474446031723417	26.52299993135702	24.302464282985216
34	24.3755172453987	25.49943726290896	26.57543720592292	23.549608285769423
35	24.634159924468385	25.521190166902596	26.45108900478807	23.39356090384095
36	23.707425237960166	25.908742971934362	26.1768488621042	24.206982928001263
37	24.333295774356024	25.813119480287366	25.816875378018157	24.036709367338453
38	24.065650547605973	25.794339505341362	26.05000984343754	24.090000103615132
39	24.598924247938445	25.215545433234343	25.84822648557008	24.337303833257135
40	25.02324762148987	25.527960607261548	26.172547861607363	23.276243909641224
41	24.013888960840486	25.589801638078857	26.83493842957867	23.561370971501987
42	24.79967777488535	26.33063560458005	25.90376503468971	22.96592158584489
43	23.827217414503625	25.61031836277971	26.433616788708754	24.12884743400791
44	23.548701265062114	26.12674451132961	25.847778111628006	24.47677611198027
45	23.64740515485147	26.28078023433969	25.910245335288106	24.161569275520737
46	23.79891625309531	25.499523400022795	25.894143000714898	24.807417346166996
47	24.382779606051304	25.536210824329032	26.0187714260922	24.06223814352746
48	24.42943841353962	25.894210072344954	25.307783364027486	24.36856815008794
49	24.089998989823115	26.14441385133823	25.535588013603714	24.22999914523494
50	23.96850805689168	26.584245739729113	25.26245444443581	24.184791758943398
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	232.0
1	139.5
2	47.0
3	132.5
4	218.0
5	242.5
6	267.0
7	235.0
8	203.0
9	303.0
10	403.0
11	588.5
12	774.0
13	1336.0
14	1898.0
15	2540.0
16	3182.0
17	3254.5
18	3327.0
19	3336.0
20	3345.0
21	3607.5
22	3870.0
23	4356.5
24	4843.0
25	5376.0
26	5909.0
27	7720.5
28	9532.0
29	11447.0
30	13362.0
31	15103.0
32	16844.0
33	20025.5
34	23207.0
35	26541.5
36	29876.0
37	32388.0
38	34900.0
39	35822.0
40	36744.0
41	38216.5
42	39689.0
43	40569.0
44	41449.0
45	44977.5
46	48506.0
47	51115.5
48	53725.0
49	55250.5
50	56776.0
51	55280.5
52	53785.0
53	52835.0
54	51885.0
55	51294.5
56	50704.0
57	48672.0
58	46640.0
59	42482.0
60	38324.0
61	33825.0
62	29326.0
63	25467.5
64	21609.0
65	18263.0
66	14917.0
67	12663.0
68	10409.0
69	9303.0
70	8197.0
71	6466.5
72	4736.0
73	4067.5
74	3399.0
75	2621.0
76	1843.0
77	1565.0
78	1287.0
79	1065.5
80	844.0
81	684.5
82	525.0
83	423.0
84	321.0
85	224.5
86	128.0
87	93.5
88	59.0
89	41.5
90	24.0
91	21.5
92	19.0
93	18.0
94	17.0
95	14.0
96	11.0
97	8.0
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009583679770406115
2	2.5901837217313825E-4
3	1.2950918608656912E-4
4	0.0
5	0.0
6	2.5901837217313825E-4
7	1.2950918608656912E-4
8	0.0
9	0.001036073488692553
10	0.001036073488692553
11	0.0
12	1.2950918608656912E-4
13	9.065643026059839E-4
14	6.475459304328456E-4
15	0.004532821513029919
16	7.770551165194148E-4
17	0.0
18	3.885275582597074E-4
19	1.2950918608656912E-4
20	3.885275582597074E-4
21	0.0
22	2.5901837217313825E-4
23	0.0019426377912985366
24	1.2950918608656912E-4
25	0.0014246010469522604
26	0.00582791337389561
27	0.006216440932155318
28	0.004273803140856781
29	0.003108220466077659
30	0.004014784768683643
31	0.0071230052347613015
32	0.0028492020939045208
33	0.004532821513029919
34	0.0034967480243373664
35	0.002460674535644813
36	0.008159078723453854
37	0.0034967480243373664
38	0.007511532793021009
39	0.004532821513029919
40	0.003108220466077659
41	0.0028492020939045208
42	0.0019426377912985366
43	0.001036073488692553
44	0.0012950918608656912
45	0.002460674535644813
46	0.0012950918608656912
47	0.0019426377912985366
48	0.0015541102330388296
49	5.180367443462765E-4
50	0.0015541102330388296
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	772146.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.82737911623526
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.94772134853409	55.431788213863285
2	11.373530664364413	15.201264911952157
3	2.80079198057344	5.61508762534478
4	1.0281776269526703	2.748416643007888
5	0.5160216333984653	1.7242186663649104
6	0.29574143770761446	1.1858175106840334
7	0.19642605518593775	0.9188646920752047
8	0.12856106694481473	0.6873119328247067
9	0.09932754260658753	0.5974019411808993
>10	0.5061925701255329	6.326221343079597
>50	0.0582342948943123	2.764222487118675
>100	0.04752095654781196	6.1116625594947775
>500	0.0017528221643417143	0.6877214730090702
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	893	0.11565170317530624	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	1.2950918608656912E-4
3	0.0	0.0	0.0	0.0	1.2950918608656912E-4
4	0.0	0.0	0.0	1.2950918608656912E-4	1.2950918608656912E-4
5	0.0	0.0	0.0	1.2950918608656912E-4	1.2950918608656912E-4
6	0.0	0.0	0.0	1.2950918608656912E-4	2.5901837217313825E-4
7	0.0	0.0	0.0	2.5901837217313825E-4	2.5901837217313825E-4
8	0.0	0.0	0.0	2.5901837217313825E-4	2.5901837217313825E-4
9	0.0	0.0	0.0	2.5901837217313825E-4	2.5901837217313825E-4
10	0.0	0.0	0.0	2.5901837217313825E-4	3.8852755825970734E-4
11	0.0	0.0	0.0	2.5901837217313825E-4	3.8852755825970734E-4
12	0.0	0.0	0.0	2.5901837217313825E-4	5.180367443462765E-4
13	0.0	0.0	0.0	2.5901837217313825E-4	6.475459304328456E-4
14	0.0	0.0	0.0	2.5901837217313825E-4	6.475459304328456E-4
15	0.0	0.0	0.0	2.5901837217313825E-4	6.475459304328456E-4
16	0.0	0.0	0.0	2.5901837217313825E-4	6.475459304328456E-4
17	0.0	0.0	0.0	2.5901837217313825E-4	6.475459304328456E-4
18	0.0	0.0	0.0	2.5901837217313825E-4	6.475459304328456E-4
19	0.0	0.0	0.0	2.5901837217313825E-4	6.475459304328456E-4
20	0.0	0.0	0.0	3.8852755825970734E-4	6.475459304328456E-4
21	0.0	0.0	0.0	9.065643026059839E-4	6.475459304328456E-4
22	0.0	0.0	0.0	0.0016836194191253986	7.770551165194147E-4
23	0.0	0.0	0.0	0.002331165349558244	0.001165582674779122
24	0.0	0.0	0.0	0.004273803140856781	0.001165582674779122
25	0.0	0.0	0.0	0.0047918398852030576	0.001165582674779122
26	0.0	0.0	0.0	0.006086931746068749	0.0014246010469522604
27	0.0	0.0	0.0	0.007641041979107578	0.0014246010469522604
28	1.2950918608656912E-4	0.0	0.0	0.012562391050397205	0.0014246010469522604
29	1.2950918608656912E-4	0.0	0.0	0.01877883198255252	0.0014246010469522604
30	1.2950918608656912E-4	0.0	0.0	0.034708461871200524	0.0014246010469522604
31	1.2950918608656912E-4	0.0	0.0	0.0666972308345831	0.0014246010469522604
32	1.2950918608656912E-4	0.0	0.0	0.10321882131099559	0.0014246010469522604
33	1.2950918608656912E-4	0.0	0.0	0.1371502280656767	0.0014246010469522604
34	1.2950918608656912E-4	0.0	0.0	0.1749669104029549	0.0014246010469522604
35	1.2950918608656912E-4	0.0	0.0	0.22236727251063917	0.0014246010469522604
36	1.2950918608656912E-4	0.0	0.0	0.28673333799566403	0.0014246010469522604
37	1.2950918608656912E-4	0.0	0.0	0.38023897035016696	0.0014246010469522604
38	1.2950918608656912E-4	0.0	0.0	0.4982218388750314	0.0014246010469522604
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGAGT	60	2.8715623E-4	21.999903	4
ATTATAC	60	2.8728205E-4	21.998478	3
GTTATAC	90	4.0805753E-7	21.998478	3
ACATTAG	75	5.469538E-5	20.533243	1
GTATAGT	75	5.469538E-5	20.533243	1
GTATCAA	2080	0.0	19.355684	1
TATATCA	430	0.0	18.418524	4
TACGTCT	60	0.007409928	18.333252	4
TTAACGG	60	0.0074126963	18.332066	35
ATAACCC	60	0.0074126963	18.332066	3
CGGTCCG	85	1.4307429E-4	18.116394	39
ATTGCAC	85	1.4307429E-4	18.116394	3
TAATACT	160	1.0986696E-9	17.87492	4
TCTATAC	75	0.0012908959	17.598783	3
GATATAC	365	0.0	17.479376	1
GGTCCGC	130	7.313665E-7	16.921907	40
TTATACT	105	3.7716392E-5	16.761831	4
GTGTATA	475	0.0	16.67361	1
GAGTAGT	80	0.0019866729	16.500996	13
TTATACC	80	0.0019875332	16.499926	4
>>END_MODULE