FastQCFastQC Report
Thu 2 Feb 2017
SRR4064169_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064169_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268516
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTT40041.4911588136274934No Hit
GTATCAACGCAGAGTACTTTTTTTT34531.2859568889749586No Hit
TATCAACGCAGAGTACTTTTTTTTT32051.1935974020170121No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25900.9645607710527492No Hit
ACGCAGAGTACTTTTTTTTTTTTTT19700.7336620536578826No Hit
GTACATGGGGTGGTATCAACGCAAA19370.7213722832158977No Hit
GGTATCAACGCAGAGTACTTTTTTT13740.511701351129914No Hit
GTACATGGAAGCAGTGGTATCAACG12300.45807326192852565No Hit
TCCATGTACTCTGCGTTGATACCAC11180.4163625258830014No Hit
GTATCAACGCAGAGTACATGGGGTG9420.3508170835257489No Hit
GTACATGGGTGGTATCAACGCAAAA9290.34597565880617914No Hit
GCAGAGTACTTTTTTTTTTTTTTTT8710.3243754562111755No Hit
TATCAACGCAGAGTACATGGGGTGG7890.2938372387492738No Hit
CCATAGGGTCTTCTCGTCTTATTAT7630.2841543893101342No Hit
GTACATGGGAGTGGTATCAACGCAA7570.28191988559340975No Hit
GTCCTAAAGTGTGTATTTCTCATTT6880.2562230928510778No Hit
GTATTAGAGGCACTGCCTGCCCAGT6810.25361617184823254No Hit
CTTTAGGACGTGAAATATGGCGAGG5980.22270553710021004No Hit
CTCTAATACTTGTAATGCTAGAGGT5910.22009861609736478No Hit
GTCCTACAGTGGACATTTCTAAATT5890.21935378152512325No Hit
CCATTGGGATGTCCTGATCCAACAT5780.21525719137779498No Hit
ATCAACGCAGAGTACTTTTTTTTTT5510.20520192465253465No Hit
CTGTAGGACGTGGAATATGGCAAGA5460.2033398382219309No Hit
GTACATGGGAAGCAGTGGTATCAAC5440.20259500364968938No Hit
GGTATCAACGCAGAGTACATGGGGT5310.19775357893011963No Hit
CATGTACTCTGCGTTGATACCACTG5250.1955190752133951No Hit
CCCATGTACTCTGCGTTGATACCAC5130.19105006777994607No Hit
GTATCAACGCAGAGTACATGGGAAG5030.18732589491873855No Hit
CTTCTACACCATTGGGATGTCCTGA4840.18024996648244423No Hit
ACGCAGAGTACATGGGGTGGTATCA4700.1750361244767537No Hit
ATCTATAACTTTATAGATGCAACAC4600.1713119516155462No Hit
GATATATTTTGATCAACGGACCAAG4450.1657256923237349No Hit
GAGTACATGGAAGCAGTGGTATCAA4430.16498085775149338No Hit
GTATCAACGCAGAGTACATGGGAGA4170.15529800831235382No Hit
GTATCCTGACCGTGCAAAGGTAGCA4130.15380833916787082No Hit
GTTATATAATTTAAGCTCCATAGGG4130.15380833916787082No Hit
GTCCTACAGTGTGCATTTCTCATTT4100.15269108730950856No Hit
GTATCAACGCAGAGTACATGGGAGT3980.1482220798760595No Hit
GTACATGGGAGAAATCGTAAATAGA3950.14710482801769728No Hit
GCCTAAAGGAAAGATCCAAAAAGAT3860.1437530724426105No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3840.143008237870369No Hit
ACCTATAACTTCTCTGTTAACCCAA3790.14114615143976522No Hit
ATCGTAAATAGATAGAAACCGACCT3780.14077373415364447No Hit
TATCAACGCAGAGTACATGGGAAGC3720.13853923043691996No Hit
CCTCTAGCATTACAAGTATTAGAGG3720.13853923043691996No Hit
ATCCTGACCGTGCAAAGGTAGCATA3670.1366771440063162No Hit
TCCCAATGGTGTAGAAGCTATTAAT3640.13555989214795394No Hit
GGTATCAACGCAGAGTACATGGGAG3560.13258055385898793No Hit
GTCAGGATACCGCGGCCGTTAAACT3550.13220813657286717No Hit
GATAGAAACCGACCTGGATTGCTCC3540.13183571928674642No Hit
CTGTTAGTATGAGTAACAAGAATTC3540.13183571928674642No Hit
GTCCTTCAGTGTGCATTTCTCATTT3480.1296012155700219No Hit
CTATAGAACTAGTACCGCAAGGGAA3450.12848396371165963No Hit
GATTAAACCTTGTACCTTTTGCATA3410.12699429456717665No Hit
TGCTAGAGGTGATGTTTTTGGTAAA3320.12364253899208985No Hit
ATTTAGAAATGTCCACTGTAGGACG3290.1225252871337276No Hit
CCTATAACTTCTCTGTTAACCCAAC3250.12103561798924459No Hit
GGTATCAACGCAGAGTACATGGGAA3220.11991836613088235No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3190.11880111427252008No Hit
GTATCAACGCAGAGTACATGGGTGG3170.11805627970027856No Hit
GAGTACATGGGGTGGTATCAACGCA3090.11507694141131256No Hit
GTTATAGATTAACCCAATTTTAAGT3060.11395968955295029No Hit
GTGTAGAAGCTATTAATGGTTCGTT3010.11209760312234653No Hit
GCGTTGATACCACTGCTTCCATGTA2990.11135276855010502No Hit
GATATACACTGTTCTACAAATCCCG2970.11060793397786352No Hit
ATAAATAATCCACCTATAACTTCTC2950.10986309940562201No Hit
CTGTTAACCCAACACCGGAATGCCT2940.10949068211950128No Hit
GATTAAAGATAAGAGACAGTTGGAC2930.10911826483338051No Hit
TATCAACGCAGAGTACATGGGAGTG2880.10725617840277675No Hit
GCTTCCATGTACTCTGCGTTGATAC2860.10651134383053525No Hit
ATTTAAGAGTTCATATCGACAATTA2770.10315958825544848No Hit
CACTATAAATAATCCACCTATAACT2750.10241475368320696No Hit
TATCAACGCAGAGTACATGGGTGGT2730.10166991911096546No Hit
CAACGCAGAGTACTTTTTTTTTTTT2710.10092508453872395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGAA607.6383E-817.4120582
TCCTATA350.002167746716.2814042
CCACCTT651.8977335E-716.0756613
AATCGTA601.4569978E-615.83209213
CCCAATT551.1222201E-515.54423513
AAATGTC755.776019E-815.2016397
AAGCTAT755.7892976E-815.19880715
GAAATGT704.391004E-714.93018156
CTTATTA1151.8189894E-1214.868418
TTCCGGT456.7310676E-414.77937211
TCCGGTG456.7310676E-414.77937212
GTTCTAT456.740588E-414.7766191
CCGGTGT456.740588E-414.77661913
TCTCGTC1203.6379788E-1214.25153812
CTTCTCG1203.6379788E-1214.25153810
ACTTGCT400.005260292414.2515378
AAACAGC400.005260292414.2515376
CCAACGT400.005260292414.25153719
CGTCTTA1203.6379788E-1214.24888315
TCGTCTT1203.6379788E-1214.24888314
Produced by FastQC (version 0.11.5)