##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064169_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	268516
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.680942662634628	32.0	32.0	32.0	32.0	32.0
2	30.282176853520834	32.0	32.0	32.0	21.0	32.0
3	30.313679631753786	32.0	32.0	32.0	21.0	32.0
4	30.34686573612001	32.0	32.0	32.0	21.0	32.0
5	30.338311311057815	32.0	32.0	32.0	21.0	32.0
6	33.890691802350695	36.0	36.0	36.0	32.0	36.0
7	33.583790165204306	36.0	36.0	36.0	21.0	36.0
8	33.39390948770278	36.0	36.0	36.0	21.0	36.0
9	33.66129020244604	36.0	36.0	36.0	32.0	36.0
10	33.187713208896305	36.0	36.0	36.0	21.0	36.0
11	33.79877176779037	36.0	36.0	36.0	32.0	36.0
12	33.45596165591622	36.0	36.0	36.0	21.0	36.0
13	33.598925203712255	36.0	36.0	36.0	27.0	36.0
14	33.44994711674537	36.0	36.0	36.0	21.0	36.0
15	33.50032027886606	36.0	36.0	36.0	21.0	36.0
16	33.580412340419194	36.0	36.0	36.0	27.0	36.0
17	33.4325291602735	36.0	36.0	36.0	21.0	36.0
18	33.54321530188145	36.0	36.0	36.0	27.0	36.0
19	33.44167572882063	36.0	36.0	36.0	21.0	36.0
20	33.2369914641958	36.0	36.0	36.0	21.0	36.0
21	33.127474712866274	36.0	36.0	36.0	21.0	36.0
22	33.15386792593365	36.0	36.0	36.0	21.0	36.0
23	33.03107449835392	36.0	36.0	36.0	21.0	36.0
24	32.97359561441404	36.0	36.0	36.0	21.0	36.0
25	32.17487226087086	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	3.0
5	3.0
6	12.0
7	5.0
8	16.0
9	22.0
10	27.0
11	12.0
12	19.0
13	15.0
14	141.0
15	298.0
16	437.0
17	596.0
18	691.0
19	913.0
20	1122.0
21	1343.0
22	1771.0
23	2073.0
24	2453.0
25	3010.0
26	3931.0
27	5255.0
28	6658.0
29	8786.0
30	11910.0
31	16335.0
32	24055.0
33	34610.0
34	65058.0
35	76936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.87910286501993	20.553630639693008	13.101225736746022	21.466040758541038
2	12.47565167840476	23.12448091054343	43.87502374310519	20.52484366794662
3	17.34782803003218	31.406045167441306	30.869011440829457	20.377115361697058
4	9.122633217632249	18.045272394118612	40.22338254353688	32.60871184471226
5	9.21994532466275	43.323128263573864	35.043613637548695	12.413312774214694
6	25.21901072705602	40.744189511323	21.401594159713948	12.635205601907032
7	24.86729520623722	35.39539509709335	23.17426141633731	16.563048280332122
8	26.567791400757773	34.55791132520425	21.60949858244014	17.26479869159784
9	28.124767114304028	14.465741582579405	22.71511185965749	34.69437944345908
10	15.200411406084635	28.5498680817446	34.91958203526764	21.330138476903127
11	31.067147131927957	24.155676706999415	27.190135307861716	17.587040853210905
12	23.677845645862757	28.231549839563826	32.685750062422905	15.404854452150515
13	27.743967203950433	23.57961427373521	29.593589863039227	19.082828659275133
14	20.8012671200969	24.102114972514673	31.486443678375103	23.61017422901332
15	21.606564585670153	31.710570935609972	29.244752169749095	17.43811230897078
16	19.207477276369286	29.025874566303813	31.48103289569304	20.285615261633865
17	17.19132570351019	29.133949234719548	32.1142013841011	21.560523677669167
18	17.465068000462157	27.471515838200272	36.74559366695613	18.317822494381435
19	20.81125000931786	28.0005367086343	32.81265141519631	18.375561866851534
20	21.880136718240408	26.872141608489354	33.74234500855421	17.50537666471603
21	23.017233668266794	25.68651420734071	30.79772801812815	20.498524106264348
22	21.162053067264004	29.001382775315783	30.751655789579612	19.084908367840598
23	19.398484470105	29.34633920883823	32.78330457081729	18.47187175023948
24	19.871491452019875	29.837239898773426	32.423064451807434	17.86820419739927
25	19.568766306373462	29.19828550130451	33.31084606783452	17.922102124487512
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	254.0
1	254.0
2	601.5
3	949.0
4	949.0
5	949.0
6	2925.5
7	4902.0
8	4902.0
9	4902.0
10	4338.5
11	3775.0
12	3775.0
13	3775.0
14	2894.0
15	2013.0
16	2013.0
17	2013.0
18	3440.0
19	4867.0
20	4867.0
21	4867.0
22	7813.5
23	10760.0
24	10760.0
25	10760.0
26	16175.5
27	21591.0
28	21591.0
29	21591.0
30	24715.5
31	27840.0
32	27840.0
33	27840.0
34	28641.0
35	29442.0
36	29442.0
37	29442.0
38	28732.5
39	28023.0
40	28023.0
41	28023.0
42	27932.5
43	27842.0
44	27842.0
45	27842.0
46	34402.0
47	40962.0
48	40962.0
49	40962.0
50	35829.0
51	30696.0
52	30696.0
53	30696.0
54	22676.5
55	14657.0
56	14657.0
57	14657.0
58	11511.0
59	8365.0
60	8365.0
61	8365.0
62	6544.5
63	4724.0
64	4724.0
65	4724.0
66	3970.5
67	3217.0
68	3217.0
69	3217.0
70	2479.5
71	1742.0
72	1742.0
73	1742.0
74	1385.0
75	1028.0
76	1028.0
77	1028.0
78	738.0
79	448.0
80	448.0
81	448.0
82	319.0
83	190.0
84	190.0
85	190.0
86	123.0
87	56.0
88	56.0
89	56.0
90	40.0
91	24.0
92	24.0
93	24.0
94	17.0
95	10.0
96	10.0
97	10.0
98	74.5
99	139.0
100	139.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03947623232879977
2	0.006331093864052793
3	0.0014896691444830105
4	0.002979338288966021
5	0.008193180294656557
6	0.013407022300347094
7	0.023462289025607413
8	0.036124476753713004
9	0.053628089201388375
10	0.06256610406828643
11	0.061448852209924174
12	0.06889719793233923
13	0.07113170164906375
14	0.07113170164906375
15	0.06219368678216568
16	0.06815236336009772
17	0.06815236336009772
18	0.07783521279923729
19	0.07969729922984105
20	0.08453872394941084
21	0.07597312636863353
22	0.0800697165159618
23	0.08453872394941084
24	0.07709037822699578
25	0.08044213380208255
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	268516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.23222452293346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.41266767480063	24.788466981483413
2	14.505162153635265	9.930879351695989
3	5.059889685483959	5.196338393242861
4	2.4151698778272177	3.307065500752283
5	1.2695960573983616	2.1730548645145915
6	0.7343421925825999	1.5082900087890478
7	0.516759320706274	1.2382874763515024
8	0.3600996529553193	0.9861609736477529
9	0.25892361753282783	0.7977178268706521
>10	1.8755643555739294	13.144468113631962
>50	0.30135227754871136	7.169032757824486
>100	0.26001153189220944	17.332672913345945
>500	0.02067037282825096	4.651119486362079
>1k	0.009791229234434665	7.7764453514874345
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACTTTTTTTTTTTTTTTTTTTTT	4004	1.4911588136274934	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3453	1.2859568889749586	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3205	1.1935974020170121	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2590	0.9645607710527492	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1970	0.7336620536578826	No Hit
GTACATGGGGTGGTATCAACGCAAA	1937	0.7213722832158977	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1374	0.511701351129914	No Hit
GTACATGGAAGCAGTGGTATCAACG	1230	0.45807326192852565	No Hit
TCCATGTACTCTGCGTTGATACCAC	1118	0.4163625258830014	No Hit
GTATCAACGCAGAGTACATGGGGTG	942	0.3508170835257489	No Hit
GTACATGGGTGGTATCAACGCAAAA	929	0.34597565880617914	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	871	0.3243754562111755	No Hit
TATCAACGCAGAGTACATGGGGTGG	789	0.2938372387492738	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	763	0.2841543893101342	No Hit
GTACATGGGAGTGGTATCAACGCAA	757	0.28191988559340975	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	688	0.2562230928510778	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	681	0.25361617184823254	No Hit
CTTTAGGACGTGAAATATGGCGAGG	598	0.22270553710021004	No Hit
CTCTAATACTTGTAATGCTAGAGGT	591	0.22009861609736478	No Hit
GTCCTACAGTGGACATTTCTAAATT	589	0.21935378152512325	No Hit
CCATTGGGATGTCCTGATCCAACAT	578	0.21525719137779498	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	551	0.20520192465253465	No Hit
CTGTAGGACGTGGAATATGGCAAGA	546	0.2033398382219309	No Hit
GTACATGGGAAGCAGTGGTATCAAC	544	0.20259500364968938	No Hit
GGTATCAACGCAGAGTACATGGGGT	531	0.19775357893011963	No Hit
CATGTACTCTGCGTTGATACCACTG	525	0.1955190752133951	No Hit
CCCATGTACTCTGCGTTGATACCAC	513	0.19105006777994607	No Hit
GTATCAACGCAGAGTACATGGGAAG	503	0.18732589491873855	No Hit
CTTCTACACCATTGGGATGTCCTGA	484	0.18024996648244423	No Hit
ACGCAGAGTACATGGGGTGGTATCA	470	0.1750361244767537	No Hit
ATCTATAACTTTATAGATGCAACAC	460	0.1713119516155462	No Hit
GATATATTTTGATCAACGGACCAAG	445	0.1657256923237349	No Hit
GAGTACATGGAAGCAGTGGTATCAA	443	0.16498085775149338	No Hit
GTATCAACGCAGAGTACATGGGAGA	417	0.15529800831235382	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	413	0.15380833916787082	No Hit
GTTATATAATTTAAGCTCCATAGGG	413	0.15380833916787082	No Hit
GTCCTACAGTGTGCATTTCTCATTT	410	0.15269108730950856	No Hit
GTATCAACGCAGAGTACATGGGAGT	398	0.1482220798760595	No Hit
GTACATGGGAGAAATCGTAAATAGA	395	0.14710482801769728	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	386	0.1437530724426105	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	384	0.143008237870369	No Hit
ACCTATAACTTCTCTGTTAACCCAA	379	0.14114615143976522	No Hit
ATCGTAAATAGATAGAAACCGACCT	378	0.14077373415364447	No Hit
TATCAACGCAGAGTACATGGGAAGC	372	0.13853923043691996	No Hit
CCTCTAGCATTACAAGTATTAGAGG	372	0.13853923043691996	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	367	0.1366771440063162	No Hit
TCCCAATGGTGTAGAAGCTATTAAT	364	0.13555989214795394	No Hit
GGTATCAACGCAGAGTACATGGGAG	356	0.13258055385898793	No Hit
GTCAGGATACCGCGGCCGTTAAACT	355	0.13220813657286717	No Hit
GATAGAAACCGACCTGGATTGCTCC	354	0.13183571928674642	No Hit
CTGTTAGTATGAGTAACAAGAATTC	354	0.13183571928674642	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	348	0.1296012155700219	No Hit
CTATAGAACTAGTACCGCAAGGGAA	345	0.12848396371165963	No Hit
GATTAAACCTTGTACCTTTTGCATA	341	0.12699429456717665	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	332	0.12364253899208985	No Hit
ATTTAGAAATGTCCACTGTAGGACG	329	0.1225252871337276	No Hit
CCTATAACTTCTCTGTTAACCCAAC	325	0.12103561798924459	No Hit
GGTATCAACGCAGAGTACATGGGAA	322	0.11991836613088235	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	319	0.11880111427252008	No Hit
GTATCAACGCAGAGTACATGGGTGG	317	0.11805627970027856	No Hit
GAGTACATGGGGTGGTATCAACGCA	309	0.11507694141131256	No Hit
GTTATAGATTAACCCAATTTTAAGT	306	0.11395968955295029	No Hit
GTGTAGAAGCTATTAATGGTTCGTT	301	0.11209760312234653	No Hit
GCGTTGATACCACTGCTTCCATGTA	299	0.11135276855010502	No Hit
GATATACACTGTTCTACAAATCCCG	297	0.11060793397786352	No Hit
ATAAATAATCCACCTATAACTTCTC	295	0.10986309940562201	No Hit
CTGTTAACCCAACACCGGAATGCCT	294	0.10949068211950128	No Hit
GATTAAAGATAAGAGACAGTTGGAC	293	0.10911826483338051	No Hit
TATCAACGCAGAGTACATGGGAGTG	288	0.10725617840277675	No Hit
GCTTCCATGTACTCTGCGTTGATAC	286	0.10651134383053525	No Hit
ATTTAAGAGTTCATATCGACAATTA	277	0.10315958825544848	No Hit
CACTATAAATAATCCACCTATAACT	275	0.10241475368320696	No Hit
TATCAACGCAGAGTACATGGGTGGT	273	0.10166991911096546	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	271	0.10092508453872395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.724172861207526E-4	0.0
10	0.0	0.0	0.0	3.724172861207526E-4	0.0
11	0.0	0.0	0.0	3.724172861207526E-4	0.0
12	0.0	0.0	0.0	3.724172861207526E-4	0.0
13	0.0	0.0	0.0	3.724172861207526E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGAA	60	7.6383E-8	17.412058	2
TCCTATA	35	0.0021677467	16.281404	2
CCACCTT	65	1.8977335E-7	16.07566	13
AATCGTA	60	1.4569978E-6	15.832092	13
CCCAATT	55	1.1222201E-5	15.544235	13
AAATGTC	75	5.776019E-8	15.201639	7
AAGCTAT	75	5.7892976E-8	15.198807	15
GAAATGT	70	4.391004E-7	14.9301815	6
CTTATTA	115	1.8189894E-12	14.8684	18
TTCCGGT	45	6.7310676E-4	14.779372	11
TCCGGTG	45	6.7310676E-4	14.779372	12
GTTCTAT	45	6.740588E-4	14.776619	1
CCGGTGT	45	6.740588E-4	14.776619	13
TCTCGTC	120	3.6379788E-12	14.251538	12
CTTCTCG	120	3.6379788E-12	14.251538	10
ACTTGCT	40	0.0052602924	14.251537	8
AAACAGC	40	0.0052602924	14.251537	6
CCAACGT	40	0.0052602924	14.251537	19
CGTCTTA	120	3.6379788E-12	14.248883	15
TCGTCTT	120	3.6379788E-12	14.248883	14
>>END_MODULE